##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295254_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4731785
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.33854158631468	31.0	30.0	33.0	26.0	34.0
2	30.38938201968179	31.0	30.0	34.0	26.0	34.0
3	30.308619474468937	31.0	30.0	34.0	26.0	34.0
4	33.35846451180686	35.0	33.0	37.0	26.0	37.0
5	33.809849559944084	35.0	35.0	37.0	28.0	37.0
6	34.00420581239427	37.0	35.0	37.0	28.0	37.0
7	33.91403265363917	37.0	35.0	37.0	28.0	37.0
8	33.93981066341772	37.0	35.0	37.0	28.0	37.0
9	34.92936703590717	39.0	35.0	39.0	27.0	39.0
10	34.89641033986117	38.0	35.0	39.0	27.0	39.0
11	35.402546185002066	39.0	35.0	39.0	28.0	39.0
12	35.36796895886013	39.0	35.0	39.0	27.0	39.0
13	35.337313297201796	39.0	35.0	39.0	27.0	39.0
14	36.035899348765845	39.0	36.0	41.0	27.0	41.0
15	36.41259397880504	40.0	36.0	41.0	27.0	41.0
16	36.437574192403076	40.0	36.0	41.0	27.0	41.0
17	36.14485675067654	40.0	36.0	41.0	27.0	41.0
18	36.05519481548718	39.0	36.0	41.0	27.0	41.0
19	36.44151667922359	40.0	36.0	41.0	27.0	41.0
20	36.43090060093601	40.0	36.0	41.0	27.0	41.0
21	36.48073950950857	40.0	36.0	41.0	27.0	41.0
22	36.42327176741969	40.0	36.0	41.0	27.0	41.0
23	36.18217691632228	40.0	36.0	41.0	27.0	41.0
24	36.43612907179848	40.0	36.0	41.0	27.0	41.0
25	35.25451346584851	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.21695411663559128
1101	2	0.15505438162036
1101	3	-0.2068564333534546
1101	4	-0.35565985311396986
1101	5	-0.3843867328605981
1101	6	-0.550911690231942
1101	7	-0.4673853516922861
1101	8	-0.35655143415812063
1101	9	-0.15370982659794663
1101	10	5.657287649754039E-4
1101	11	0.0027523696530806774
1101	12	-0.06792104376814478
1101	13	0.014936839682050618
1101	14	0.2909264821245259
1101	15	0.15196107775468448
1101	16	0.06087894065277055
1101	17	0.24330369421805642
1101	18	0.37299134465008166
1101	19	0.0788353163556792
1101	20	0.06112633416283586
1101	21	-0.037005978878916324
1101	22	0.04579416863920471
1101	23	0.02679826989240297
1101	24	-0.0748953139561408
1101	25	-0.1256099141440501
1102	1	-0.33593691994051866
1102	2	0.005823393337404781
1102	3	-0.2880330019904953
1102	4	-0.48243318835712756
1102	5	-0.846238521004409
1102	6	-0.6873787487101737
1102	7	-0.7698718268184805
1102	8	-0.6905502204844112
1102	9	-0.4922105878616776
1102	10	-0.1400864577980343
1102	11	-0.2914763496525765
1102	12	-0.3533789562109817
1102	13	-0.4376153601175403
1102	14	-0.2681775521362866
1102	15	-0.3499402165108876
1102	16	-0.45249491667896535
1102	17	-0.4588880802969726
1102	18	-0.16810069738898648
1102	19	-0.30271541505645416
1102	20	-0.21282346186367107
1102	21	-0.3001671449009109
1102	22	-0.40584319315727413
1102	23	-0.31649935875456237
1102	24	-0.46372359020907794
1102	25	-0.4514357355532681
1103	1	-0.5157526880621681
1103	2	-0.16066131500778624
1103	3	-0.6100768256424374
1103	4	-0.7133179058499408
1103	5	-0.7595631205350344
1103	6	-0.7960149591995247
1103	7	-0.7821330572392142
1103	8	-0.6811038235047278
1103	9	-0.31210245409601356
1103	10	-0.19593470335650665
1103	11	-0.36571945492096347
1103	12	-0.4439204080674486
1103	13	-0.4284533404191677
1103	14	-0.41605858623510983
1103	15	-0.4664897960521657
1103	16	-0.49440220466882323
1103	17	-0.3100454918271751
1103	18	-0.09934102348701401
1103	19	-0.32922674635925375
1103	20	-0.3737494922597051
1103	21	-0.25496289732616617
1103	22	-0.3555238219994976
1103	23	-2.410656946387057
1103	24	-1.174967526878433
1103	25	-0.7023630021431586
1104	1	-0.33431336635505104
1104	2	-0.047437292729220815
1104	3	-0.3178882645937584
1104	4	-0.8148959973870973
1104	5	-0.5509509325764412
1104	6	-0.6569313623697255
1104	7	-0.5519538710305198
1104	8	-0.5198200911273361
1104	9	-2.7931285002391064
1104	10	-1.049106178968259
1104	11	-0.7068534266731206
1104	12	-0.5721419911644219
1104	13	-0.4747408368730959
1104	14	-0.27723021916771273
1104	15	-0.3779557327322678
1104	16	-0.38365117380264024
1104	17	-0.10223382372735301
1104	18	0.036753039554568545
1104	19	-0.2890660349356651
1104	20	-0.3701988154654501
1104	21	-0.3067335423454409
1104	22	-0.43933969693241437
1104	23	-0.24252675687666425
1104	24	-0.20752344546539092
1104	25	-0.31278172602517884
1105	1	-0.22025912443108453
1105	2	-0.14950550075695546
1105	3	-0.2594912325796983
1105	4	-0.08087842987213634
1105	5	-0.23060441295781686
1105	6	-0.291750673397388
1105	7	-0.37685114310033896
1105	8	-0.2966610017525184
1105	9	-2.135877609207661
1105	10	-0.6150367978456259
1105	11	-0.46946883698778663
1105	12	-0.4188586312576916
1105	13	-0.37968717624048054
1105	14	-0.18441696940411845
1105	15	-0.21028118230503878
1105	16	-0.2881193316346611
1105	17	-1.5435698805943687
1105	18	-0.40187037907917755
1105	19	-0.3247748416392895
1105	20	-0.37914855059928243
1105	21	-0.1401657696702685
1105	22	-0.2058403886839102
1105	23	-0.07273802400804641
1105	24	-0.15735068416392295
1105	25	-0.7167457503484229
1106	1	-0.38150904354040094
1106	2	-0.47076961176813015
1106	3	-0.5319509515112593
1106	4	-0.5980057393894285
1106	5	-0.4600182634864538
1106	6	-0.5194096010581504
1106	7	-0.571998806915083
1106	8	-0.5939174015866797
1106	9	-3.2316593230700477
1106	10	-0.9412271826576628
1106	11	-0.7429366677492695
1106	12	-0.491865682100908
1106	13	-0.6014023519807381
1106	14	-0.863121270528957
1106	15	-0.7659973146944452
1106	16	-0.8213454448337671
1106	17	-0.7350313798270847
1106	18	-0.3656732206328783
1106	19	-0.5460861662270418
1106	20	-0.5283030383895024
1106	21	-0.44423102600215003
1106	22	-0.5140370302605461
1106	23	-0.3129583091735668
1106	24	-0.5618577469278634
1106	25	-0.7662061772967164
1107	1	-0.1553502111531877
1107	2	-0.013267403249923149
1107	3	-0.07619767948049017
1107	4	-1.3792719649616174
1107	5	-0.2560547786595109
1107	6	-0.1194184746175253
1107	7	0.029036002498742164
1107	8	0.0012175226496538016
1107	9	0.34124245367171824
1107	10	0.42774216274649035
1107	11	0.14084589647247014
1107	12	-0.42481078133744177
1107	13	-0.03863589840847226
1107	14	0.2494123285031904
1107	15	-0.49889649048325424
1107	16	-0.8932209980050487
1107	17	-0.03327436943241935
1107	18	0.06215784513101141
1107	19	0.18988756295752296
1107	20	-0.20851575300557812
1107	21	-0.042767110940530983
1107	22	0.043101102386586376
1107	23	0.1653079489204572
1107	24	-0.04517600096457386
1107	25	-0.20593686080108142
1108	1	0.4316048110259736
1108	2	0.3500123462922744
1108	3	0.3572225581051178
1108	4	0.8305056332114802
1108	5	0.54226415663544
1108	6	0.47797069182144725
1108	7	0.415368619090124
1108	8	0.4252572264548533
1108	9	0.7821908505380435
1108	10	0.8633313939360718
1108	11	0.6018009659551709
1108	12	0.5347137767279406
1108	13	0.5497950637827032
1108	14	0.6007006668027515
1108	15	0.5759941072719954
1108	16	0.6333152005180693
1108	17	0.6603758398272177
1108	18	0.6925099889024651
1108	19	0.5291722342631147
1108	20	0.5213301715299821
1108	21	0.4351385223716733
1108	22	0.5868555340068156
1108	23	0.6678455388189093
1108	24	0.48699755049845095
1108	25	0.6520200238681468
1109	1	0.29921253048900454
1109	2	0.23207912569077394
1109	3	0.3367557223844315
1109	4	0.6880076305064406
1109	5	0.5576280447554396
1109	6	0.5036147959422834
1109	7	0.47815178347876497
1109	8	0.45875463751455214
1109	9	0.8306873673518282
1109	10	0.5698145412269042
1109	11	0.41873970639340996
1109	12	0.44006985058172177
1109	13	0.3454809766328637
1109	14	0.748395965518057
1109	15	0.5606841800054525
1109	16	0.5543958168961964
1109	17	0.8185327224815993
1109	18	0.6118524710929805
1109	19	0.3445921996914407
1109	20	0.45163359856891816
1109	21	0.4346729897574946
1109	22	0.48618714531551177
1109	23	0.6747888401299278
1109	24	0.46444084678070396
1109	25	0.4450994438370728
1110	1	0.3927025431431659
1110	2	0.24449193459288665
1110	3	0.42264999642869583
1110	4	0.6384202296895367
1110	5	0.6052583243376191
1110	6	0.5811819551144168
1110	7	0.45278007741458026
1110	8	0.5962455207950583
1110	9	1.0670788133226594
1110	10	1.0107478827484684
1110	11	0.625677679279498
1110	12	0.5179087755169363
1110	13	0.5740587099045342
1110	14	0.7808172383787877
1110	15	0.5842822480839232
1110	16	0.606783676755434
1110	17	0.993255866228111
1110	18	1.0520582129656333
1110	19	0.6471681424139177
1110	20	0.6487148947661083
1110	21	0.6266522818848017
1110	22	0.6381918209633284
1110	23	0.7178005722794794
1110	24	0.4935086206969217
1110	25	0.623677958332955
1111	1	0.2032253650891711
1111	2	0.1957163501918444
1111	3	0.21195963933398332
1111	4	0.6814583397420861
1111	5	0.5680050652958784
1111	6	0.4131543970994258
1111	7	0.28322835712980066
1111	8	0.22864603353112045
1111	9	0.5938882786883255
1111	10	0.5513328334861569
1111	11	0.33953679297911066
1111	12	0.14548640300541393
1111	13	0.1359468258537717
1111	14	0.16014741330072724
1111	15	0.1867959011058673
1111	16	0.07437313333807083
1111	17	0.42563736932415
1111	18	0.5400217639958811
1111	19	0.3528741781204232
1111	20	-0.20719789862496896
1111	21	-0.3224146444164617
1111	22	-0.053835563206177994
1111	23	0.21556077176471433
1111	24	0.1455934977239295
1111	25	0.19605481189777407
1112	1	0.2630889630804809
1112	2	0.08597864862035465
1112	3	0.33259858467703296
1112	4	0.7842078571879583
1112	5	0.6591363049910228
1112	6	0.529829295765289
1112	7	0.4797093095974745
1112	8	0.46675290655109336
1112	9	-1.078002247841674
1112	10	0.20903732888996984
1112	11	0.17360920214069608
1112	12	0.2634895751214543
1112	13	0.19139812746050922
1112	14	0.5027846885996254
1112	15	0.33237009944583207
1112	16	0.28752673359236525
1112	17	0.38527144767409993
1112	18	0.3477474246687322
1112	19	0.3224694636649943
1112	20	0.2809717729707941
1112	21	0.20517605710269038
1112	22	0.33319936598249456
1112	23	0.03511411836687728
1112	24	0.24394542750108172
1112	25	0.45125154129063816
1113	1	0.24717425009059468
1113	2	0.12606765401963926
1113	3	0.298692707990579
1113	4	0.6925984375292487
1113	5	0.6602954317264462
1113	6	0.5700890285707914
1113	7	0.41915573025710273
1113	8	0.49975232817841686
1113	9	0.28730866654890974
1113	10	0.5844400281904072
1113	11	0.4376253360027036
1113	12	0.17131100304737856
1113	13	0.24029192866346705
1113	14	0.04241835985435927
1113	15	0.1776653015416514
1113	16	0.2571049538556309
1113	17	0.6630145901232893
1113	18	0.4361409896865638
1113	19	0.321194076206595
1113	20	0.309020649369522
1113	21	0.30723202784797365
1113	22	0.4038557212674547
1113	23	0.046985901352073256
1113	24	0.18880316198992375
1113	25	0.2510580258057331
1114	1	0.37595393957955636
1114	2	0.225314213354725
1114	3	0.38179385600590265
1114	4	0.7842007356952934
1114	5	0.6191028339754965
1114	6	0.5188301503444066
1114	7	0.31915169672181776
1114	8	0.38491627359282177
1114	9	0.20837789743677604
1114	10	0.4065519279499341
1114	11	0.3890129907747948
1114	12	0.43622494045403215
1114	13	0.3326778596923816
1114	14	0.7207735790710643
1114	15	0.38387273439811764
1114	16	0.24691642167098138
1114	17	0.6612043632132014
1114	18	0.4954543040306447
1114	19	0.4057232736635683
1114	20	0.36674303062639524
1114	21	0.3510491521033998
1114	22	0.16884955826279935
1114	23	0.627892924804712
1114	24	0.4528188073615027
1114	25	0.27033002013980223
1115	1	0.27655356331674597
1115	2	0.1497988011621345
1115	3	0.3954177895549442
1115	4	0.85806794062281
1115	5	0.65031799248667
1115	6	0.5082734950695809
1115	7	0.48444672228487207
1115	8	0.4508870128785034
1115	9	0.6156256490282388
1115	10	0.37340766451399077
1115	11	0.4253652339855307
1115	12	0.3397464485084498
1115	13	0.2754189183971789
1115	14	0.3641813275438537
1115	15	0.3794920014797398
1115	16	0.45216836326505927
1115	17	0.6336833229605858
1115	18	0.7672370476304948
1115	19	0.5139237382889448
1115	20	0.4751107632747775
1115	21	0.41111844163662425
1115	22	-0.16302629811049485
1115	23	0.27294065069595064
1115	24	0.42174889842403473
1115	25	0.4496073385381294
1116	1	0.6172985944603155
1116	2	0.4682957384467663
1116	3	0.764498091635609
1116	4	1.2629462993955798
1116	5	1.096251329779669
1116	6	0.9623655909892079
1116	7	0.886138993149558
1116	8	0.7925682686838726
1116	9	1.1604758111320876
1116	10	0.9803603461395909
1116	11	0.6613138962774627
1116	12	0.6637084390775314
1116	13	0.5838901718752112
1116	14	1.0287854534284264
1116	15	0.7511684987684077
1116	16	0.8026064943102114
1116	17	1.1189044527684118
1116	18	1.1545905937915037
1116	19	0.8501210978036724
1116	20	0.7820370702296984
1116	21	0.8565899660607457
1116	22	0.8996814266498134
1116	23	1.1146221695557799
1116	24	0.8283121902266046
1116	25	0.7349142517892773
1201	1	-0.28074088174312095
1201	2	0.1378671384721315
1201	3	-0.3109084947288743
1201	4	-0.40955600566645955
1201	5	-0.5786300657839334
1201	6	-0.7158592534312191
1201	7	-0.6399491903820191
1201	8	-0.6151320745763798
1201	9	-0.1683186394661007
1201	10	-0.15042651351635072
1201	11	-0.17889750698095241
1201	12	-0.16339185422540226
1201	13	-0.1316089711382915
1201	14	0.1937071999413149
1201	15	-0.0812987899480575
1201	16	-0.12615297461137942
1201	17	-0.0991028886931673
1201	18	0.1134713698001093
1201	19	-0.1455117668492889
1201	20	-0.18826332659072165
1201	21	-0.13534890220179818
1201	22	-0.2121028404125198
1201	23	-0.31055273117963367
1201	24	-0.373241476141672
1201	25	-0.27254734458723107
1202	1	-0.4271564384744302
1202	2	-0.26409233614398175
1202	3	-0.5469111021151818
1202	4	-0.6388977164100496
1202	5	-0.9602127624600314
1202	6	-0.8016659450405186
1202	7	-0.6767480148316949
1202	8	-0.6433576436351416
1202	9	-0.12635430340356635
1202	10	-0.20876877313154552
1202	11	-0.3478666598317375
1202	12	-0.4246216177695956
1202	13	-0.3148760815007918
1202	14	-0.39292748753648965
1202	15	-0.40977273207715115
1202	16	-0.3374607272482777
1202	17	0.03895142120772732
1202	18	0.08670509100870305
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2211	15	0.04635090774447548
2211	16	0.116267123094552
2211	17	0.34413991901859475
2211	18	0.5089837672328343
2211	19	0.0948208849265626
2211	20	0.20866208820854837
2211	21	0.12463601433932325
2211	22	0.15333763621019614
2211	23	-1.91355917721134
2211	24	-0.6483221199258438
2211	25	-0.36423116624298046
2212	1	0.04828870184371681
2212	2	-1.463344871506326
2212	3	-0.26489390762526455
2212	4	0.44079419168391354
2212	5	0.45317799575688866
2212	6	0.43372758301775605
2212	7	0.409613949323294
2212	8	0.29941762671156624
2212	9	0.7810887135162616
2212	10	0.5971634402561321
2212	11	0.3925571135563146
2212	12	0.21011391512722355
2212	13	0.2281802966800015
2212	14	0.6135102090900801
2212	15	0.32184936117956653
2212	16	0.368181755697627
2212	17	0.5966911081450164
2212	18	0.6810850520972878
2212	19	0.4086531692400115
2212	20	0.3594826323994127
2212	21	0.34186776131655705
2212	22	0.35207511200211883
2212	23	0.4516610730375916
2212	24	0.303393117488973
2212	25	0.2230091683723856
2213	1	0.2907009529244249
2213	2	0.0911408715198192
2213	3	0.27164086873570525
2213	4	0.58030634652701
2213	5	0.5272138040771637
2213	6	0.43810086728515785
2213	7	0.30725571658432926
2213	8	0.43632467087587656
2213	9	0.5886023143035075
2213	10	0.6670039395213649
2213	11	0.3156627499766813
2213	12	0.3297187785237128
2213	13	0.09650627284412394
2213	14	-1.307785445681212
2213	15	-0.27203158602635824
2213	16	0.043029413287641205
2213	17	0.4283137402954509
2213	18	0.24666977579607163
2213	19	0.16515796704320707
2213	20	0.257579991137149
2213	21	0.34602170993492365
2213	22	0.3333702275359798
2213	23	0.48787799921737474
2213	24	0.23217997992220063
2213	25	0.15427794050113874
2214	1	0.43407402657936345
2214	2	0.36622912619166215
2214	3	0.5213196818539174
2214	4	0.8762887826160721
2214	5	0.6947653711872732
2214	6	0.5997476752377082
2214	7	0.48756311392850904
2214	8	0.4041241675020899
2214	9	0.6663886332175366
2214	10	0.5286144385828919
2214	11	0.41050798061011307
2214	12	0.27877843175949124
2214	13	0.246471522133362
2214	14	0.44359444527707126
2214	15	0.2897661865571024
2214	16	0.4423678348450295
2214	17	0.37482234195128683
2214	18	0.6217152656454488
2214	19	0.37917358244185095
2214	20	0.511873579026485
2214	21	0.5253875600435975
2214	22	0.5191811061181326
2214	23	0.4770149359048048
2214	24	0.48063133618147447
2214	25	0.48817967338359836
2215	1	0.3184146120220426
2215	2	0.2008113176876023
2215	3	0.3700426720816026
2215	4	0.8001099644511669
2215	5	0.6059230419489694
2215	6	0.5592149387896086
2215	7	0.562188032696497
2215	8	0.4994917661693705
2215	9	0.7200176357674266
2215	10	0.7175094657823351
2215	11	0.47446728848982644
2215	12	0.3742233036806226
2215	13	0.31037373297531445
2215	14	0.4239995770810978
2215	15	0.29598124666362224
2215	16	0.3673629995817649
2215	17	0.5716237997986653
2215	18	0.6675611688027558
2215	19	0.24698182603030006
2215	20	-0.5558385463915201
2215	21	0.0636624208008314
2215	22	-0.6403440896345742
2215	23	0.21324278723619017
2215	24	0.21236270276372693
2215	25	0.26298957663119893
2216	1	0.13227845750193623
2216	2	-0.3036416901782353
2216	3	0.27160683743955616
2216	4	0.751363628541796
2216	5	0.49020623119759676
2216	6	0.4701661252398637
2216	7	0.4350744814270371
2216	8	0.39922791445983563
2216	9	0.7093082305289471
2216	10	0.5469118874529499
2216	11	0.4235223904204446
2216	12	-0.008745638755875973
2216	13	0.1691051543908202
2216	14	0.24568745578373097
2216	15	0.19606589918814166
2216	16	0.31094191819581596
2216	17	0.3089671736322117
2216	18	0.6124548057631927
2216	19	0.3134589143693063
2216	20	-0.7728421552722295
2216	21	-1.0354316756657056
2216	22	-1.0673431092744323
2216	23	-1.5380466029843305
2216	24	-1.2453479089557788
2216	25	-1.0150074061962258
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10181.0
3	1387.0
4	1151.0
5	816.0
6	977.0
7	1373.0
8	1002.0
9	2116.0
10	3825.0
11	6468.0
12	10445.0
13	15895.0
14	21664.0
15	26518.0
16	30016.0
17	31585.0
18	32262.0
19	32751.0
20	32307.0
21	32319.0
22	32552.0
23	33515.0
24	35195.0
25	37134.0
26	41156.0
27	45743.0
28	53129.0
29	64135.0
30	79455.0
31	100772.0
32	131529.0
33	176683.0
34	242419.0
35	345179.0
36	513339.0
37	839279.0
38	1665513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06905107529955	21.8616582401249	14.27374894112597	22.795541743449586
2	15.437920572009729	22.0042597190739	40.352550256672664	22.20526945224371
3	17.442112519221592	27.438612863884615	30.747059759266133	24.372214857627657
4	13.066304068557011	18.61950345303413	38.93082496232543	29.383367516083432
5	13.055531626968078	38.42616894450317	35.36336396994079	13.15493545858796
6	29.71879560607928	39.52049313579792	18.034710302443134	12.726000955679668
7	24.745422634166	33.8141904968954	24.329177956530266	17.111208912408333
8	23.33117452432839	37.0106696642504	21.891914309212584	17.766241502208622
9	24.38537012267781	17.521920084435116	22.658085710298124	35.434624082588954
10	14.638766530105283	30.704754767678384	34.33392536572729	20.322553336489047
11	31.015981222106426	26.29867569380433	24.5008942055986	18.184448878490645
12	20.451748219414988	28.23257756343052	31.876061399614798	19.439612817539693
13	25.853679739961795	25.131598755785873	27.260650986063023	21.754070518189316
14	20.45199496487457	24.501067928388572	28.653943700918976	26.392993405817876
15	22.236626531889847	31.665657940709824	24.787143973302598	21.31057155409773
16	21.438406791204308	31.76577079346205	26.232099533991004	20.563722881342635
17	19.949028090765978	30.741207674467102	28.772887560355194	20.536876674411733
18	19.90112781978805	30.62543620547311	29.615740488414406	19.857695486324435
19	20.681909914488184	29.564644171624295	29.75964767867562	19.993798235211898
20	21.906060275180828	29.173323212909473	28.97580533166827	19.94481118024143
21	22.359314792147128	29.521659794127125	27.99003193657566	20.128993477150086
22	21.211482613299026	29.122369013005944	29.48825530034774	20.17789307334729
23	21.56705262497435	29.467221524128327	28.583489768543718	20.3822360823536
24	21.34182208815056	28.5766449825123	29.09968561581005	20.981847313527087
25	21.711193956745078	29.078393446835904	28.509134812539255	20.701277783879764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13983.0
1	13983.0
2	12063.0
3	10143.0
4	10143.0
5	10143.0
6	11113.0
7	12083.0
8	12083.0
9	12083.0
10	17302.5
11	22522.0
12	22522.0
13	22522.0
14	36220.5
15	49919.0
16	49919.0
17	49919.0
18	79581.0
19	109243.0
20	109243.0
21	109243.0
22	155723.0
23	202203.0
24	202203.0
25	202203.0
26	259066.0
27	315929.0
28	315929.0
29	315929.0
30	374240.5
31	432552.0
32	432552.0
33	432552.0
34	478317.0
35	524082.0
36	524082.0
37	524082.0
38	549688.5
39	575295.0
40	575295.0
41	575295.0
42	567682.0
43	560069.0
44	560069.0
45	560069.0
46	536452.5
47	512836.0
48	512836.0
49	512836.0
50	483640.5
51	454445.0
52	454445.0
53	454445.0
54	408887.0
55	363329.0
56	363329.0
57	363329.0
58	311414.0
59	259499.0
60	259499.0
61	259499.0
62	211491.5
63	163484.0
64	163484.0
65	163484.0
66	124672.5
67	85861.0
68	85861.0
69	85861.0
70	62602.0
71	39343.0
72	39343.0
73	39343.0
74	27555.0
75	15767.0
76	15767.0
77	15767.0
78	10862.0
79	5957.0
80	5957.0
81	5957.0
82	4081.0
83	2205.0
84	2205.0
85	2205.0
86	1486.5
87	768.0
88	768.0
89	768.0
90	488.5
91	209.0
92	209.0
93	209.0
94	126.0
95	43.0
96	43.0
97	43.0
98	29.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1569597942425533
2	0.29804819956950707
3	0.3600121307286785
4	0.38873279322708026
5	0.39492495960826624
6	0.355383856198031
7	0.3594837888872804
8	0.3754608461711595
9	0.36263270626201316
10	0.3939739442937496
11	0.3117005527512344
12	0.35720135213244053
13	0.3287342937179098
14	0.34052688361791583
15	0.37867316456686007
16	0.32898789780178095
17	0.37651752985395576
18	0.34667678265178997
19	0.3592301848034093
20	0.3222885232528528
21	0.3619775623786795
22	0.3174066446383341
23	0.2996120914200455
24	0.336004277455548
25	0.3307631263888786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4731785.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.82147446365326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.49391671145409	63.30631873807907
2	11.983050086241235	18.171450522212357
3	2.747085376742039	6.248641912263655
4	0.7837129800219197	2.3768909480626226
5	0.33231377085368496	1.2598260045351497
6	0.17213566903284916	0.7830948140314825
7	0.09982707258530352	0.5298325084365494
8	0.07221544068793362	0.43803849536012995
9	0.05197263369617742	0.35465775467431687
>10	0.24074487278235163	3.2243382019685423
>50	0.014401905103394386	0.7595750911834582
>100	0.007532246476011558	1.0624251993437734
>500	5.875879961768727E-4	0.3344989804966618
>1k	3.917249209040538E-4	0.617381063421348
>5k	1.119214059710447E-4	0.533029765930849
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6751	0.14267343085114814	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6627	0.14005285531781347	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5940	0.12553402151619314	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5807	0.12272324291995515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.1133673655924774E-5	0.0
4	0.0	0.0	0.0	1.2680204193554864E-4	0.0
5	0.0	0.0	0.0	1.2680204193554864E-4	0.0
6	0.0	0.0	0.0	1.2680204193554864E-4	0.0
7	0.0	0.0	0.0	1.2680204193554864E-4	0.0
8	0.0	0.0	0.0	1.4793571559147342E-4	0.0
9	0.0	0.0	0.0	1.690693892473982E-4	0.0
10	0.0	0.0	0.0	2.9587143118294684E-4	0.0
11	0.0	0.0	0.0	3.381387784947964E-4	0.0
12	0.0	0.0	0.0	3.381387784947964E-4	0.0
13	0.0	0.0	0.0	3.381387784947964E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	1870	0.0	10.879674	1
GGTATCA	3830	0.0	10.549549	1
TGGTATC	1845	0.0	10.510583	2
ACACCGC	105	4.115054E-5	9.951053	6
ACGGCAG	80	0.004494872	9.505995	5
CCTACAC	500	0.0	8.745516	3
GCGTCAG	220	1.5861588E-9	8.642732	1
TACACCG	135	6.753873E-5	8.449774	5
ACTAGAC	340	0.0	8.387644	3
TATACAG	960	0.0	8.317746	5
CGTCAGA	240	9.240466E-10	8.317658	2
GTATCAA	6720	0.0	8.262041	1
TATTCCG	210	5.7547368E-8	8.147996	5
GTCTTGG	635	0.0	8.084697	1
CTAGTAC	295	1.2732926E-11	8.055929	3
GTCTCGC	260	5.18412E-10	8.04439	1
GTCTAGA	475	0.0	8.005898	1
TCTGGAC	540	0.0	7.921663	3
GGACACG	120	0.0016941295	7.91561	6
CAGTGCG	145	1.5660496E-4	7.860936	9
>>END_MODULE