FastQCFastQC Report
Wed 25 May 2016
SRR1295254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731785
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT182680.38606995034643377No Hit
GGTATCAACGCAGAGTACTTTTTTT157220.33226361721844927No Hit
TATCAACGCAGAGTACTTTTTTTTT102070.21571140700602415No Hit
GTGGTATCAACGCAGAGTACATGGG72380.1529655299215835No Hit
ACGCAGAGTACTTTTTTTTTTTTTT69500.14687903190867718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTTA400.005288272614.24713215
AGCGTCA4450.012.3837183
GGTATCA52750.012.2491581
GTGGTAT18950.011.9841841
TGGTATC20350.011.1587752
CAGCGTC5100.010.99172
GCAGCGT5100.09.8747361
TTACACT7050.09.8381214
CTGTTCG950.00182560468.9996159
CGCAGTA850.0074310978.9424012
GTATAGG5150.08.67182351
CTTACAC6300.08.5964153
GTCTTAG4900.08.5325011
GCGTCAG6800.08.1039654
GTCTAGA4700.08.0868971
ATAATAC8950.08.0681433
GTATCAA91200.08.0642851
AGGACCG1304.1810647E-48.0398175
GTTCTAG4800.07.91841941
GTCCTAC3850.07.89785341
Produced by FastQC (version 0.11.5)