FastQCFastQC Report
Wed 25 May 2016
SRR1295253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693564
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG45450.16873554888615974No Hit
GTATCAACGCAGAGTACTTTTTTTT38050.1412626542380281No Hit
GGTATCAACGCAGAGTACTTTTTTT32040.11895020871974826No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30100.11174785525794079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27230.1010928271984627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.011.9691321
GTGGTAT8950.011.89734651
TGGTATC8900.011.5340982
TAAACCG752.0630835E-411.4070045
CGTCAGA951.3532683E-511.0057332
GTCCTAA2050.010.66667651
ACCCCGC750.00265194210.1318136
ACCATCG750.002652761810.1314368
CTAGTAC1554.2286956E-79.1986823
CCAATAC2501.8189894E-129.12509253
ATACCGT1054.5009024E-49.0462616
CACAGCG950.00182684958.998657
GCGTCAG850.007391278.9480051
GTCCTAG2502.5465852E-118.7466741
TATACTG5000.08.745375
GTCTAAT1856.497612E-88.7363961
ATTAGAC2850.08.6715063
AAACCGT1000.00290977958.5487176
GTCTTAG1803.9892257E-78.4508931
CTATACT4400.08.4259414
Produced by FastQC (version 0.11.5)