FastQCFastQC Report
Wed 25 May 2016
SRR1295253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693564
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113050.419704154050173No Hit
GGTATCAACGCAGAGTACTTTTTTT96950.3599320454238325No Hit
TATCAACGCAGAGTACTTTTTTTTT63790.23682377697355622No Hit
GTGGTATCAACGCAGAGTACATGGG47990.17816543434646437No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43420.1611990656245777No Hit
GGTATCAACGCAGAGTACATGGGGG28360.10528801246229903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3000.013.3016513
GTGGTAT11000.012.9575961
GCAGCGT3100.012.8741
GGTATCA30250.012.4078811
CAGCGTC3350.011.9117042
TGGTATC11850.011.5455222
GCGTCAG3900.010.2316594
CTAGTAC1502.442357E-810.1345913
CGTCAGA3950.010.1027085
TATTCCG1151.025295E-59.9144585
GTCTAGG1606.61621E-89.5022371
AACAGCG1201.7017994E-59.4997687
CCTATAC2151.038643E-98.8383063
ACACCGT1000.0029074568.5494746
GTCCTAG2701.4551915E-118.4464341
GTATAAC2409.295036E-108.3144581
GCTATAC1606.44158E-68.3135323
CCTAGAC1951.5336809E-78.2830793
TCTCGAT1150.00111138418.26051412
GTCCTAA2658.913048E-118.2472261
Produced by FastQC (version 0.11.5)