FastQCFastQC Report
Wed 25 May 2016
SRR1295252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4995951
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG88320.17678315900215996No Hit
GTATCAACGCAGAGTACTTTTTTTT77230.1545851830812592No Hit
GGTATCAACGCAGAGTACTTTTTTT66280.13266743408812456No Hit
ATTCCATTCCATTCCATTCCATTCC59400.11889628220933311No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54010.10810754549033808No Hit
GGTATCAACGCAGAGTACATGGGGG50270.10062148327715784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA550.00307260912.08957910
TGGTATC17800.011.8006422
GTGGTAT19300.011.7220991
GGTATCA47200.011.6606381
ATTACGC700.001486277310.8624963
TAGGACC2901.8189894E-128.5216134
GTGCTCG1356.81447E-58.4433459
TAGACAG6750.08.1669885
TAAGACC4400.07.9927374
GTATCAA79900.07.8163561
GTCTTAG5000.07.7947041
ACTCTCG1100.0068652347.7717168
CTAATAC5900.07.7326253
GTCCTAA3850.07.65396931
GTCTTAA6250.07.60458851
CGAATAC1752.1238564E-57.60374743
GTATTAG6900.07.43927151
GTCTAAT4100.07.41911131
TAAGGGT3850.07.40639644
GGACTGT5900.07.40574176
Produced by FastQC (version 0.11.5)