FastQCFastQC Report
Wed 25 May 2016
SRR1295252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4995951
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT223890.4481429061253804No Hit
GGTATCAACGCAGAGTACTTTTTTT195590.39149703429837485No Hit
TATCAACGCAGAGTACTTTTTTTTT124520.24924183603882424No Hit
GTGGTATCAACGCAGAGTACATGGG96730.1936167908772524No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80760.16165090490279027No Hit
ATTCCATTCCATTCCATTCCATTCC61230.12255924847941863No Hit
GGTATCAACGCAGAGTACATGGGGG54070.10822764274509497No Hit
GAATGGAATGGAATGGAATGGAATG53330.10674644326975984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21300.012.7136581
GGTATCA59900.012.7060621
TGGTATC21400.012.3853652
AGCGTCA5150.012.3591973
CAGCGTC5850.011.0426022
CGCAGTA909.5327414E-510.55458318
GCAGCGT5600.010.3501611
CTAGTAC2401.8189894E-129.499983
GTACCGT1356.854427E-69.1482216
AGTACCG1252.7562508E-59.1201635
TACACCG1252.7562508E-59.1201635
CCGCTCG1054.493277E-49.0481439
ACTCGCG1201.7641406E-48.70883758
GCGTCAG7350.08.6597594
GACTGCG1651.0506319E-68.6367787
GTATCAA111200.08.630221
TACCCGC1000.00290547158.55040911
GTCCTAC2701.4551915E-118.44603351
GTGTAAC3600.08.44603351
GTCTTGC7700.08.3911891
Produced by FastQC (version 0.11.5)