FastQCFastQC Report
Wed 25 May 2016
SRR1295251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364272
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG65080.1491199448613652No Hit
GTATCAACGCAGAGTACTTTTTTTT54200.12419024295460962No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50770.11633097112187325No Hit
GGTATCAACGCAGAGTACTTTTTTT46530.10661571964350526No Hit
ATTCCATTCCATTCCATTCCATTCC45780.10489721997162414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.010.9788991
GTGGTAT15300.010.8733741
TAATACG1002.383826E-510.4568384
TAGGACG1659.2040864E-1010.3704184
TGGTATC15400.09.9375162
TTAGGAC3050.09.66153
ACTGTCG800.0045229919.4987818
GACGTCC1106.8519046E-59.498787
AGACGTC1752.6795533E-89.2273876
TTAGACG1151.1036196E-49.0929034
GTACCGT1054.5013535E-49.0464576
CTAGGAC4750.08.8052523
TAGGACT5200.08.7749694
TACCGCC1000.00290995538.54890257
TATTCCG1901.0023541E-78.5053665
TAGACTG4750.08.4053035
TGCCCGC1951.5417936E-78.28079810
TGGACGG1150.00110433058.2660855
ACCGTTT1401.0414612E-48.1418128
TGTAACG1304.1627022E-48.0430742
Produced by FastQC (version 0.11.5)