##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295251_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4364272
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.65886521280067	31.0	30.0	33.0	27.0	34.0
2	30.541627332118622	31.0	30.0	34.0	26.0	34.0
3	30.742701646460166	31.0	31.0	34.0	27.0	34.0
4	34.068849054321085	37.0	35.0	37.0	28.0	37.0
5	34.33754518508471	37.0	35.0	37.0	30.0	37.0
6	34.44614451161614	37.0	35.0	37.0	30.0	37.0
7	34.27795861486177	37.0	35.0	37.0	30.0	37.0
8	34.41448883112693	37.0	35.0	37.0	30.0	37.0
9	35.44282391198349	39.0	35.0	39.0	28.0	39.0
10	35.42847948065565	39.0	35.0	39.0	28.0	39.0
11	35.89219462031697	39.0	35.0	39.0	30.0	39.0
12	35.88060987032889	39.0	35.0	39.0	30.0	39.0
13	35.83238945693577	39.0	35.0	39.0	30.0	39.0
14	36.551505039099304	40.0	36.0	41.0	27.0	41.0
15	36.91930040107491	40.0	37.0	41.0	30.0	41.0
16	36.906955845098565	40.0	37.0	41.0	30.0	41.0
17	36.599447972078735	40.0	36.0	41.0	27.0	41.0
18	36.49475376420168	40.0	36.0	41.0	27.0	41.0
19	36.91457521437711	40.0	37.0	41.0	29.0	41.0
20	36.92379622534984	40.0	37.0	41.0	29.0	41.0
21	36.97221163117239	40.0	37.0	41.0	30.0	41.0
22	36.87773997587685	40.0	37.0	41.0	29.0	41.0
23	36.61844335091855	40.0	36.0	41.0	27.0	41.0
24	36.89081844577973	40.0	37.0	41.0	29.0	41.0
25	35.72137254506593	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10320411687140663
1101	2	0.16725289540979915
1101	3	9.781422886341318E-4
1101	4	0.10060289030332825
1101	5	-0.15799808337985155
1101	6	-0.26043493407885876
1101	7	-0.3189933654474828
1101	8	-0.21031520289889727
1101	9	-0.07179157523129476
1101	10	-0.02628199454269975
1101	11	-0.02343055794401039
1101	12	0.00908742450885569
1101	13	-0.02186576959864084
1101	14	0.4167885793230255
1101	15	0.275217179402361
1101	16	0.04065298140876905
1101	17	0.16831257195956795
1101	18	0.31278797630529453
1101	19	0.07654018355673742
1101	20	0.14276954070348324
1101	21	0.07487652980607606
1101	22	-0.014171314013104563
1101	23	0.047877421669298315
1101	24	-0.08564251486509988
1101	25	-0.052582995444410585
1102	1	-0.14484341552801894
1102	2	-0.014283031966751736
1102	3	-0.07484525290640676
1102	4	-0.016976181649042132
1102	5	-0.36461069731154794
1102	6	-0.41136146872507595
1102	7	-0.5336155453711058
1102	8	-0.36953828329471605
1102	9	-0.2248794871851203
1102	10	0.01694386779975332
1102	11	-0.0743292487208862
1102	12	-0.21642571042776382
1102	13	-0.1944001229790686
1102	14	-0.0755349317864571
1102	15	-0.050962890634359326
1102	16	-0.19035349759851528
1102	17	-0.244065601960898
1102	18	0.08373371614447933
1102	19	-0.19061327496419267
1102	20	-0.19978409107903872
1102	21	-0.06634130393600657
1102	22	-0.13540418002821042
1102	23	-0.07472855463534955
1102	24	-0.16887745513865582
1102	25	-0.2586055682569821
1103	1	-0.3172130247769438
1103	2	-0.1183382446361243
1103	3	-0.3355789307051644
1103	4	-0.10814276716956783
1103	5	-0.3988840787875816
1103	6	-0.4942671224174333
1103	7	-0.5213358556650789
1103	8	-0.3809870827497619
1103	9	0.0016741410128773282
1103	10	0.18134924644719064
1103	11	-0.057199106090656926
1103	12	-0.17472145819142781
1103	13	-0.22161081538305893
1103	14	-0.19315389736979682
1103	15	-0.28266508747208974
1103	16	-0.23896628401177367
1103	17	-0.03954536877119352
1103	18	0.1625886396338032
1103	19	-0.0802036419245411
1103	20	-0.035192755925471886
1103	21	-0.03549444849588923
1103	22	-0.0189397299858598
1103	23	-2.2635466936109268
1103	24	-0.9721721264275516
1103	25	-0.4422230951588588
1104	1	-0.2858374860542199
1104	2	-0.08344762774957104
1104	3	-0.27686923614212944
1104	4	-0.4794441879639919
1104	5	-0.41025751209711103
1104	6	-0.47288561673803997
1104	7	-0.5444224351230744
1104	8	-0.4576253130600989
1104	9	-2.810388317181868
1104	10	-0.8327626570897095
1104	11	-0.5397520609486861
1104	12	-0.4381480944275751
1104	13	-0.24090505212292612
1104	14	-0.11396837942576354
1104	15	-0.2595576676297213
1104	16	-0.29865644821991566
1104	17	0.03912375456710748
1104	18	0.14039086127706923
1104	19	-0.1745304763775124
1104	20	-0.26912609477413696
1104	21	-0.2435485235124304
1104	22	-0.2162940407610705
1104	23	-0.07822189962104176
1104	24	-0.15670770623448504
1104	25	-0.2317982685384692
1105	1	-0.2285705837303027
1105	2	-0.16738067411511892
1105	3	-0.22148112451689883
1105	4	-0.12137586761394203
1105	5	-0.19852237412290208
1105	6	-0.3744224362391435
1105	7	-0.37467345470910374
1105	8	-0.3473905386153717
1105	9	-2.1176135189152134
1105	10	-0.6095458287295301
1105	11	-0.3862603211157136
1105	12	-0.23722274143165123
1105	13	-0.29324258765228706
1105	14	-0.23620822799739472
1105	15	-0.23039787379380527
1105	16	-0.24710019080491463
1105	17	-1.4754625154373784
1105	18	-0.3268897843923071
1105	19	-0.28684064884425453
1105	20	-0.24490546695227522
1105	21	-0.27077041986601813
1105	22	-0.19858567712962127
1105	23	-0.1613713678109292
1105	24	-0.1570767223195375
1105	25	-0.6151044906978385
1106	1	-0.19004189522942028
1106	2	-0.3342272045566297
1106	3	-0.28413056498628464
1106	4	-0.16321159985545108
1106	5	-0.17365327380097995
1106	6	-0.25880048296169633
1106	7	-0.34282168180890693
1106	8	-0.2976659184546193
1106	9	-3.103367861801722
1106	10	-0.7262716497579831
1106	11	-0.6104454161384041
1106	12	-0.2745353638582415
1106	13	-0.3980295951796009
1106	14	-0.6401175342955341
1106	15	-0.5476674240268338
1106	16	-0.5334070730505545
1106	17	-0.40143897913645077
1106	18	0.003860626037351267
1106	19	-0.19267296625613284
1106	20	-0.2956087153548097
1106	21	-0.22665392638401016
1106	22	-0.22053673855958777
1106	23	-0.046586477270111004
1106	24	-0.269989761436527
1106	25	-0.5124573162570769
1107	1	-0.22000337983900664
1107	2	-0.12392809471674582
1107	3	-0.14791165050791832
1107	4	-1.4592116695631745
1107	5	-0.3282264938929984
1107	6	-0.18278655415951306
1107	7	-0.12347396619842499
1107	8	-0.04399417661604588
1107	9	0.19566767939254248
1107	10	0.45268212727624046
1107	11	0.16333213152893933
1107	12	-0.41770509391695754
1107	13	-0.01149502839435712
1107	14	0.21589975996549526
1107	15	-0.4574688169820078
1107	16	-0.7206752761353812
1107	17	0.02211572868325362
1107	18	0.016733880908269327
1107	19	0.009590454729035969
1107	20	-0.15521530146033058
1107	21	0.004464556506114548
1107	22	0.05811126142841516
1107	23	0.32137950761006095
1107	24	-0.030040790487404934
1107	25	-0.2861852065416173
1108	1	0.2989676986173784
1108	2	0.2815736740809811
1108	3	0.3070895825481905
1108	4	0.6802282253294223
1108	5	0.42517584806322617
1108	6	0.3907186842029873
1108	7	0.4010038132801199
1108	8	0.2730065632176206
1108	9	0.6738464555205752
1108	10	0.7280280632933867
1108	11	0.48259455138028784
1108	12	0.38238972384585423
1108	13	0.3353699477098644
1108	14	0.427067038908298
1108	15	0.43160877201062675
1108	16	0.48718196998261476
1108	17	0.5525450448048232
1108	18	0.6627555316727651
1108	19	0.43997197929662235
1108	20	0.4214977111083371
1108	21	0.3586006093659577
1108	22	0.47197285300622127
1108	23	0.6124710173809689
1108	24	0.41607958980410586
1108	25	0.4751269777534972
1109	1	0.34003202152312895
1109	2	0.23261263259727016
1109	3	0.33570208603694596
1109	4	0.6708167793547517
1109	5	0.5040387137178399
1109	6	0.46773472378002623
1109	7	0.4558504154640559
1109	8	0.37537685058868675
1109	9	0.7787919900692017
1109	10	0.504681211146
1109	11	0.32312570071121627
1109	12	0.456999775750333
1109	13	0.2863282636207316
1109	14	0.8087572825638816
1109	15	0.4757319932574191
1109	16	0.46399969499828586
1109	17	0.8084688105527746
1109	18	0.5341010915755575
1109	19	0.324899316097067
1109	20	0.36009842695458616
1109	21	0.38191846779769634
1109	22	0.4528411105104908
1109	23	0.6802394520576982
1109	24	0.4444085151248416
1109	25	0.3116205783824455
1110	1	0.3819894827860111
1110	2	0.3106255103104445
1110	3	0.38788375947040876
1110	4	0.5746332120498323
1110	5	0.6141622381195972
1110	6	0.5064442977482742
1110	7	0.4906654531408492
1110	8	0.4841469360936159
1110	9	1.03593043974017
1110	10	0.9399926470232955
1110	11	0.540248963759673
1110	12	0.5833226692157254
1110	13	0.6070940182644264
1110	14	0.9073349472191978
1110	15	0.6005219203663685
1110	16	0.5616205761745192
1110	17	0.932591676965707
1110	18	0.9495874756094267
1110	19	0.597214739477387
1110	20	0.5918069034856401
1110	21	0.5984881281623444
1110	22	0.7219055032740727
1110	23	0.742642811067391
1110	24	0.5553907632928698
1110	25	0.5918625250569391
1111	1	0.17807511940544174
1111	2	0.12261440039365468
1111	3	0.2527196369772007
1111	4	0.5421221003739873
1111	5	0.45952262418717993
1111	6	0.357267719297532
1111	7	0.2489003123100133
1111	8	0.28921065231191534
1111	9	0.7141510519389058
1111	10	0.5574188805567459
1111	11	0.37351043133952544
1111	12	0.22575830288406706
1111	13	0.2358245294326693
1111	14	0.18347228001093896
1111	15	0.17684664264350403
1111	16	0.2041694772672571
1111	17	0.4737842365203804
1111	18	0.4783455597205233
1111	19	0.3586576434285149
1111	20	-0.11027252958525224
1111	21	-0.17706226002967185
1111	22	0.009398155779585693
1111	23	0.3834918163531853
1111	24	0.30039744956935976
1111	25	0.24881323273659461
1112	1	0.25159408796911364
1112	2	0.1446242690273678
1112	3	0.2558775385594032
1112	4	0.5809860713686703
1112	5	0.5160362169887946
1112	6	0.4407235849145721
1112	7	0.39123316658221796
1112	8	0.382366530284834
1112	9	-1.106073564866385
1112	10	0.18805374302208122
1112	11	0.1947494922777082
1112	12	0.204667378561318
1112	13	0.13690230220774424
1112	14	0.6106151540946243
1112	15	0.33484265357023446
1112	16	0.309927544640054
1112	17	0.4184692209952061
1112	18	0.41945304015099794
1112	19	0.3617768133585244
1112	20	0.30551688269204647
1112	21	0.2592602451450716
1112	22	0.28684690471853
1112	23	0.05426957853204328
1112	24	0.28338478651866694
1112	25	0.35769632282240593
1113	1	0.11071275688308901
1113	2	0.005537334843555897
1113	3	0.12352889270082557
1113	4	0.19249924317569622
1113	5	0.3370564210781595
1113	6	0.2274697287715739
1113	7	0.21191504218604962
1113	8	0.20390186748979033
1113	9	0.04791045118597026
1113	10	0.3604097185037034
1113	11	0.09745303090791424
1113	12	-0.02736945490913456
1113	13	0.030720576101742836
1113	14	-0.2317821732592833
1113	15	-0.10148121867734261
1113	16	0.01754601293639979
1113	17	0.44468990847927614
1113	18	0.21764349119312243
1113	19	0.11404848162559489
1113	20	0.09320074387715493
1113	21	0.07290627105876268
1113	22	0.087936657478906
1113	23	-0.24156656669673282
1113	24	-0.10724642531847195
1113	25	-0.13935849635877418
1114	1	0.3173580734643373
1114	2	0.3045798930612271
1114	3	0.28998298768082975
1114	4	0.5136195019619194
1114	5	0.4651583658293532
1114	6	0.4726703771264056
1114	7	0.2819963117604445
1114	8	0.19606055297447966
1114	9	0.047667363776064064
1114	10	0.3124351703176771
1114	11	0.3052357810149715
1114	12	0.2402312023542521
1114	13	0.26696148943867826
1114	14	0.6130334237789157
1114	15	0.2715409233846344
1114	16	0.23415787751763162
1114	17	0.5697704654233604
1114	18	0.5159480146939117
1114	19	0.29502551863784987
1114	20	0.22932562943424273
1114	21	0.2127684330854933
1114	22	0.030455543972429666
1114	23	0.44008334693634055
1114	24	0.27353428343081987
1114	25	0.20505978757408627
1115	1	0.2707414943745121
1115	2	0.19533940015265117
1115	3	0.33710583541942185
1115	4	0.5021530788256499
1115	5	0.504009660361632
1115	6	0.4092957997509501
1115	7	0.4087110234333764
1115	8	0.39371229321528034
1115	9	0.5954183602882424
1115	10	0.3582846018876751
1115	11	0.3059954670114209
1115	12	0.29811174857961475
1115	13	0.1944211596047012
1115	14	0.33597537997112426
1115	15	0.2698935219424854
1115	16	0.31257922569414376
1115	17	0.6080758680448497
1115	18	0.7446032421762894
1115	19	0.484728609528446
1115	20	0.4646115267931492
1115	21	0.3499092771608048
1115	22	-0.22047130158863837
1115	23	0.29137678142872403
1115	24	0.3493727266640718
1115	25	0.32546309680601127
1116	1	0.36486416150810186
1116	2	0.3259450742141823
1116	3	0.45817296406271524
1116	4	0.729915279348468
1116	5	0.736492072804694
1116	6	0.5451181148110962
1116	7	0.6351982847629571
1116	8	0.47858334695848015
1116	9	0.7931527927498792
1116	10	0.582195016607642
1116	11	0.3348843045218288
1116	12	0.2953091746534682
1116	13	0.22744881361002456
1116	14	0.688389081985946
1116	15	0.44521877515765595
1116	16	0.4980430837365759
1116	17	0.8982044196447276
1116	18	0.9002747614567923
1116	19	0.5096296854816202
1116	20	0.413439718031789
1116	21	0.5964913344257852
1116	22	0.6250392259505873
1116	23	0.7848646955208523
1116	24	0.4860865059395607
1116	25	0.43038883947470197
1201	1	-0.13161236535137277
1201	2	0.1398358563650426
1201	3	-0.13094826114945235
1201	4	0.08282429044495387
1201	5	-0.18220326981307977
1201	6	-0.20696983994655938
1201	7	-0.2979548578900406
1201	8	-0.19782610564107017
1201	9	0.20954608012973353
1201	10	0.280906074707417
1201	11	0.12511128270258354
1201	12	0.11601744184579843
1201	13	0.11015833119984109
1201	14	0.49231279000355954
1201	15	0.18400383727846759
1201	16	0.1332404109011165
1201	17	0.1913759128625543
1201	18	0.390116865519758
1201	19	0.14575040014648977
1201	20	0.12257756453568192
1201	21	0.09267046431637027
1201	22	0.08827776175667879
1201	23	0.02880257977737699
1201	24	-0.046393107007311585
1201	25	0.04536238096268619
1202	1	-0.25681824183710233
1202	2	-0.23932655752222942
1202	3	-0.33150110633149765
1202	4	-0.20112223396100148
1202	5	-0.5381554340339605
1202	6	-0.5202270848427375
1202	7	-0.6310404486664609
1202	8	-0.4429503384461242
1202	9	0.13524882973317887
1202	10	0.207791984660183
1202	11	-0.1072379558642993
1202	12	-0.14897740640018498
1202	13	-0.13907224659067907
1202	14	-0.07785637539291912
1202	15	-0.1142153214362267
1202	16	-0.11669620896722677
1202	17	0.2307962727493731
1202	18	0.2717426312621427
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2211	9	-1.588591631609944
2211	10	0.06322763048508762
2211	11	0.016767829843502113
2211	12	0.03918885186442367
2211	13	0.01800972032638981
2211	14	0.24316376838122977
2211	15	0.08694263707229766
2211	16	0.19620932481978315
2211	17	0.41336145333130503
2211	18	0.4668719045959904
2211	19	0.16931896783793832
2211	20	0.34686616503195467
2211	21	0.04527643873287701
2211	22	0.084258800858386
2211	23	-2.0015916324162575
2211	24	-0.5733769931186643
2211	25	-0.28038554007299155
2212	1	0.02154699002296212
2212	2	-1.5015295207824835
2212	3	-0.307553799520349
2212	4	0.18249789346587875
2212	5	0.2401360089667648
2212	6	0.2596074441627607
2212	7	0.2817450912255097
2212	8	0.24282806085821562
2212	9	0.7217991901070988
2212	10	0.5533169934395943
2212	11	0.3350643819674346
2212	12	0.22153840490101118
2212	13	0.15246440117935123
2212	14	0.5813195723654019
2212	15	0.20940756523411608
2212	16	0.2948455810292643
2212	17	0.5771841722125757
2212	18	0.7494657119156685
2212	19	0.4151040671747026
2212	20	0.40984322824301245
2212	21	0.33495243113434725
2212	22	0.3608327973088379
2212	23	0.544188933370755
2212	24	0.2794583706503957
2212	25	0.2440023935498914
2213	1	0.16001156940310324
2213	2	0.15745427325823513
2213	3	0.1047523871217031
2213	4	0.2865339978814916
2213	5	0.27053927751044426
2213	6	0.25261435049984016
2213	7	0.2910278322207702
2213	8	0.22119725073120122
2213	9	0.5379773701847128
2213	10	0.5211700457328732
2213	11	0.19929652207718362
2213	12	0.21148276102183416
2213	13	0.07939655154603287
2213	14	-1.2966602813023016
2213	15	-0.34406739929397645
2213	16	-0.0958695411137569
2213	17	0.3447842200782745
2213	18	0.05412342940545045
2213	19	-0.08410422555825647
2213	20	0.05295078076707682
2213	21	0.16926444713516986
2213	22	0.19207563882208234
2213	23	0.35485251440909593
2213	24	0.15199606489112938
2213	25	0.019715891678004027
2214	1	0.20391608773217484
2214	2	0.27189514361343115
2214	3	0.25601145126439917
2214	4	0.47559296459391476
2214	5	0.3295886904601133
2214	6	0.2746061084251039
2214	7	0.193948724431948
2214	8	0.12390316270820989
2214	9	0.3450351815583801
2214	10	0.22239216207647416
2214	11	0.005517864903389125
2214	12	-0.022690373566902622
2214	13	-0.0432796046978936
2214	14	0.25281993969326066
2214	15	-0.05374945072794901
2214	16	0.03397852031998383
2214	17	0.0333936826647232
2214	18	0.2725614509125265
2214	19	0.025861764362979045
2214	20	0.16762473495509767
2214	21	0.1378810359563616
2214	22	0.1523600463571242
2214	23	0.2061588124864926
2214	24	0.2079571684865016
2214	25	0.14042595596223606
2215	1	0.2206410843669815
2215	2	0.12945103782466205
2215	3	0.17570879244343374
2215	4	0.4568196085695817
2215	5	0.37456125614542657
2215	6	0.381735344558912
2215	7	0.4401088017351782
2215	8	0.2802090489070821
2215	9	0.5258688994849123
2215	10	0.5461679538577187
2215	11	0.3029698548526838
2215	12	0.1568325225480649
2215	13	0.20484630470476617
2215	14	0.3713695906749379
2215	15	0.16213152408760578
2215	16	0.29765401340051056
2215	17	0.4581464212887738
2215	18	0.6250170784724745
2215	19	0.25212635486737156
2215	20	-0.5992645150240747
2215	21	-0.07701201508556466
2215	22	-0.8283550085435536
2215	23	0.07970817502327066
2215	24	0.047730034154511713
2215	25	0.15374930589717906
2216	1	0.035788303729766824
2216	2	-0.2959053106759022
2216	3	0.05684997034103745
2216	4	0.2682362199393893
2216	5	0.20647117383906277
2216	6	0.26600955144633787
2216	7	0.31452211994214707
2216	8	0.14357681223524565
2216	9	0.5464274755568397
2216	10	0.2737149983462217
2216	11	0.14039350140945572
2216	12	-0.26396861374554703
2216	13	-0.12853963361312992
2216	14	-0.08441924409546431
2216	15	-0.09822559305546008
2216	16	0.051833223210074664
2216	17	0.07369377258564214
2216	18	0.43585276475995727
2216	19	0.07726918615715306
2216	20	-0.8945011855631364
2216	21	-1.2564001367373479
2216	22	-1.244341215789028
2216	23	-1.5860509745792086
2216	24	-1.378803407632013
2216	25	-1.171138300407378
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8648.0
3	1178.0
4	1098.0
5	721.0
6	871.0
7	1209.0
8	547.0
9	858.0
10	1674.0
11	3538.0
12	6397.0
13	10112.0
14	14180.0
15	17956.0
16	21234.0
17	23096.0
18	23685.0
19	24036.0
20	24539.0
21	24781.0
22	25354.0
23	26631.0
24	27796.0
25	30100.0
26	33488.0
27	38192.0
28	44237.0
29	53868.0
30	68102.0
31	87151.0
32	115685.0
33	156331.0
34	217507.0
35	312236.0
36	472205.0
37	782485.0
38	1662546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26051577193683	21.569253787513713	14.28497340364501	22.88525703690445
2	15.472143023899203	21.882083041509727	39.94258657740081	22.70318735719026
3	17.513972703651767	27.02902234414018	30.941356374815403	24.51564857739265
4	12.928570689339939	18.12462555871527	38.507869447776	30.43893430416879
5	13.479589072035674	37.88107045879106	35.09532827731059	13.544012191862679
6	30.20308419814021	38.83659966626249	17.817596676051597	13.142719459545708
7	25.34501480761595	33.02009839862548	24.35797069561193	17.27691609814664
8	23.136477568008832	37.217145353783884	21.73801365925449	17.908363418952792
9	24.36317342187558	17.303307389462255	22.408841410069435	35.92467777859273
10	15.07036934476195	30.08590782342621	33.97719735155877	20.866525480253067
11	31.47824120609944	26.088238443822124	24.16735263985925	18.266167710219186
12	20.59148521735292	27.77225214189281	31.731695967098172	19.9045666736561
13	25.849210601379035	24.895712591740626	27.188369181447563	22.066707625432773
14	20.63090370269567	23.774273395742206	28.27453499074533	27.32028791081679
15	22.46386609967281	31.12093967917567	24.543778750976188	21.871415470175332
16	22.084312949877575	31.254351244274858	25.781408197681007	20.87992760816656
17	20.368309243443942	30.426204084373403	28.488166465604586	20.717320206578062
18	20.38619056650402	30.411099041937504	29.054546917475587	20.148163474082885
19	21.150401357070418	28.954314982242252	29.34384275052312	20.55144091016421
20	22.322549002483104	28.641941032460554	28.48793060176456	20.54757936329178
21	22.782980018826475	29.168071141457474	27.534780007514037	20.514168832202014
22	21.720269283601098	28.611899709715534	29.122764521175416	20.545066485507952
23	21.969119385953906	29.130194812207577	28.224585281294562	20.676100520543955
24	21.647514818728457	28.108634433513924	28.606702775165633	21.63714797259199
25	22.293724032484505	28.399233398028972	28.033975784645342	21.273066784841177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12594.0
1	12594.0
2	10617.5
3	8641.0
4	8641.0
5	8641.0
6	9105.0
7	9569.0
8	9569.0
9	9569.0
10	13787.0
11	18005.0
12	18005.0
13	18005.0
14	28821.0
15	39637.0
16	39637.0
17	39637.0
18	63543.0
19	87449.0
20	87449.0
21	87449.0
22	126196.0
23	164943.0
24	164943.0
25	164943.0
26	214138.0
27	263333.0
28	263333.0
29	263333.0
30	315877.5
31	368422.0
32	368422.0
33	368422.0
34	417489.0
35	466556.0
36	466556.0
37	466556.0
38	504074.0
39	541592.0
40	541592.0
41	541592.0
42	540608.0
43	539624.0
44	539624.0
45	539624.0
46	516465.0
47	493306.0
48	493306.0
49	493306.0
50	466354.5
51	439403.0
52	439403.0
53	439403.0
54	394888.0
55	350373.0
56	350373.0
57	350373.0
58	299566.5
59	248760.0
60	248760.0
61	248760.0
62	203415.5
63	158071.0
64	158071.0
65	158071.0
66	122155.0
67	86239.0
68	86239.0
69	86239.0
70	63543.5
71	40848.0
72	40848.0
73	40848.0
74	28974.0
75	17100.0
76	17100.0
77	17100.0
78	11807.5
79	6515.0
80	6515.0
81	6515.0
82	4432.5
83	2350.0
84	2350.0
85	2350.0
86	1539.5
87	729.0
88	729.0
89	729.0
90	446.5
91	164.0
92	164.0
93	164.0
94	102.5
95	41.0
96	41.0
97	41.0
98	24.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14966986475636715
2	0.2824755193993408
3	0.34138568815142595
4	0.36952325611235964
5	0.3770159146817614
6	0.3378799488207884
7	0.33959844849266957
8	0.3568063585404393
9	0.3439519809947684
10	0.37477040844383663
11	0.2930614773781286
12	0.3402171083745468
13	0.3105901740313161
14	0.32161148526031375
15	0.36028918454211833
16	0.30944450758339537
17	0.3596934379891996
18	0.32873753056638083
19	0.3414773414672596
20	0.304014048620251
21	0.34548717403498225
22	0.2984002830254393
23	0.2815131595830874
24	0.31769330600842477
25	0.31262946030861505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4364272.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.57023624378239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34605632097788	57.40610412071436
2	12.486975496786593	17.624176215571037
3	3.3124556093108173	7.012823246883196
4	1.140895716186258	3.220531208831342
5	0.5186573769879689	1.8300886811810728
6	0.31519875640295625	1.3346190421861823
7	0.182893162707208	0.9034769589734142
8	0.12278333970466482	0.6931879431807026
9	0.09315664545150044	0.5916677829471597
>10	0.4465768640363117	5.443883613830218
>50	0.022555526402311885	1.0919084769525877
>100	0.010525574338128322	1.255950464572951
>500	6.185284341116094E-4	0.3054329849096452
>1k	5.5341993228811E-4	0.8954846380563652
>5k	9.766234099183765E-5	0.3906646212097714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6508	0.1491199448613652	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5420	0.12419024295460962	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5077	0.11633097112187325	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4653	0.10661571964350526	No Hit
ATTCCATTCCATTCCATTCCATTCC	4578	0.10489721997162414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.291332895841506E-5	0.0
10	2.291332895841506E-5	0.0	0.0	2.291332895841506E-5	0.0
11	2.291332895841506E-5	0.0	0.0	6.873998687524517E-5	0.0
12	2.291332895841506E-5	2.291332895841506E-5	0.0	6.873998687524517E-5	0.0
13	2.291332895841506E-5	2.291332895841506E-5	0.0	6.873998687524517E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3680	0.0	10.978899	1
GTGGTAT	1530	0.0	10.873374	1
TAATACG	100	2.383826E-5	10.456838	4
TAGGACG	165	9.2040864E-10	10.370418	4
TGGTATC	1540	0.0	9.937516	2
TTAGGAC	305	0.0	9.6615	3
ACTGTCG	80	0.004522991	9.498781	8
GACGTCC	110	6.8519046E-5	9.49878	7
AGACGTC	175	2.6795533E-8	9.227387	6
TTAGACG	115	1.1036196E-4	9.092903	4
GTACCGT	105	4.5013535E-4	9.046457	6
CTAGGAC	475	0.0	8.805252	3
TAGGACT	520	0.0	8.774969	4
TACCGCC	100	0.0029099553	8.5489025	7
TATTCCG	190	1.0023541E-7	8.505366	5
TAGACTG	475	0.0	8.405303	5
TGCCCGC	195	1.5417936E-7	8.280798	10
TGGACGG	115	0.0011043305	8.266085	5
ACCGTTT	140	1.0414612E-4	8.141812	8
TGTAACG	130	4.1627022E-4	8.043074	2
>>END_MODULE