FastQCFastQC Report
Wed 25 May 2016
SRR1295251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364272
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT153470.35165085952479586No Hit
GGTATCAACGCAGAGTACTTTTTTT136760.31336268683528434No Hit
TATCAACGCAGAGTACTTTTTTTTT86950.19923139529341893No Hit
GTGGTATCAACGCAGAGTACATGGG69850.16004960277452918No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57300.13129337493171828No Hit
ATTCCATTCCATTCCATTCCATTCC47640.10915909915788934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.012.520321
AGCGTCA3850.012.3386013
TACGTCG550.00306796412.0919685
GTGGTAT16500.011.9207271
TGGTATC16400.011.5862462
TAAATCG700.001491250210.8580925
GCGGTAT803.7661832E-410.6897821
ACCGTCC803.7733687E-410.6875778
CAGCGTC4800.010.0945182
CTGTCGC2600.09.8654579
GCAGCGT4700.09.70421
GGACCGA1356.850767E-69.1485296
GGACCCG1252.7537411E-59.1207975
TATACTG8600.09.0589325
TACATCG1054.4916637E-49.0484095
ACTGTCG950.00182471379.0000658
GAACGAT950.00182737898.99862216
GAATCGT850.0074367128.9415496
TGTCGCC2900.08.84489210
TATACCG1000.00290428088.5507475
Produced by FastQC (version 0.11.5)