FastQCFastQC Report
Wed 25 May 2016
SRR1295250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615016
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG70220.15215548548477406No Hit
GTATCAACGCAGAGTACTTTTTTTT59480.12888362683899687No Hit
GGTATCAACGCAGAGTACTTTTTTT50010.10836365464388423No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN49700.1076919343291551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC600.00588128111.0826627
GGTATCA39450.010.7947291
CGCAAGT856.6084653E-410.05711812
GTGGTAT19200.09.5056271
TACTCCG1106.799958E-59.505425
GTCTACG1451.6946797E-69.1778461
GTACTAG2501.8189894E-129.1254021
TGGTATC18600.08.8408712
GTCTAGA4700.08.6966371
TTACGCC1000.00289110268.5550654
TATTCCG1901.0034091E-78.5048495
AGTACTG5850.08.4492635
GCGTCAG2502.3283064E-108.3649521
CTTAGAC4100.08.3461313
GACCGTG2303.7052814E-98.2603687
TTATACT7650.08.20093254
CTGTACG1050.00449130968.147684
TTAGACT4900.08.147684
CTTACAC6550.08.1266823
TAGGACA5050.08.0939014
Produced by FastQC (version 0.11.5)