FastQCFastQC Report
Wed 25 May 2016
SRR1295250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615016
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174550.3782218739870024No Hit
GGTATCAACGCAGAGTACTTTTTTT150720.32658608334185624No Hit
TATCAACGCAGAGTACTTTTTTTTT99840.21633727813728057No Hit
GTGGTATCAACGCAGAGTACATGGG75720.1640731039719039No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64040.13876441598468997No Hit
ATTCCATTCCATTCCATTCCATTCC47510.10294655533155248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3600.013.1945123
TCGACCG802.0005045E-613.0632749
GGTATCA55200.012.0163881
GCAGCGT4100.010.8936051
GTGGTAT21650.010.797791
TGGTATC20650.010.6271732
CAGCGTC4600.010.5326622
CCCGCAC951.645331E-410.00005153
AAGACCG1002.7567372E-49.5002555
AGTACCG1106.840528E-59.5002555
CTAGACG800.00451891179.4998434
CGACCGC1151.110832E-49.08749610
GTCTTAG4300.09.0609351
ACAGTCG1401.0854023E-58.8219528
ATCGACC1401.0854023E-58.8219528
GCGTCAG5400.08.7961514
TCGCGTT1107.144831E-48.63687610
TAGACAG5650.08.5754515
GACCGTG1451.6899856E-58.5175627
CGCGTTG1150.00111063218.2613611
Produced by FastQC (version 0.11.5)