FastQCFastQC Report
Wed 25 May 2016
SRR1295249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046183
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG60320.14907877374799905No Hit
GTGGTATCAACGCAGAGTACATGGG59080.14601415704628287No Hit
GTATCAACGCAGAGTACTTTTTTTT46900.1159117123471677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN44240.1093376152289701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12900.011.7914791
GGTATCA33650.011.4139061
GTCGTAC855.288366E-511.1845661
TGGTATC13800.010.4006472
ACCGACC750.002652324310.1318658
CGGTGCA1002.7623345E-49.4982699
GTCGAAT950.00181279049.0065171
AGTACGG2651.8189894E-128.96719553
GCGGCTA850.00745092948.93943713
GTATTAG5750.08.9282011
GTCCTAG3000.08.5561921
TGCGACC1000.00291193238.5482311
CTACACT5900.08.3776974
TTAGACA4200.08.373864
TACACCG1951.5237492E-78.2868145
ACACCGT1951.5417754E-78.2807476
TCTACAC5750.08.2654153
GTATTAC4900.08.1487541
GTCCTAA3750.08.1125371
GTATTGG5050.08.0949671
Produced by FastQC (version 0.11.5)