FastQCFastQC Report
Wed 25 May 2016
SRR1295249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046183
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133510.3299653031017134No Hit
GGTATCAACGCAGAGTACTTTTTTT114870.2838971939726898No Hit
TATCAACGCAGAGTACTTTTTTTTT75860.1874853411227322No Hit
GGTATCAACGCAGAGTACATGGGGG66650.16472314771724364No Hit
GTGGTATCAACGCAGAGTACATGGG64970.16057108637943462No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44990.111191214040492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1001.4445322E-712.3500648
GGTATCA40750.012.2658241
GTGGTAT15600.011.7563171
TACCGTC1001.9317868E-611.4000597
TGGTATC16100.010.7394512
AGTACCG1002.3995352E-510.4505715
ACCGGTC800.0045178969.500058
CCGTGCG800.0045178969.500059
GTACCGT1651.0672011E-79.2120556
TGTACCG1451.7068778E-69.1729155
GTATTAG5550.08.9032581
CTACACT5300.08.7831744
GTATCAA74750.08.7334171
GTTCTAG4800.08.7106391
GTCCTAC2751.8189894E-128.6386511
ACGGGTC1107.144684E-48.6368365
TATACCG1107.144684E-48.6368365
GTGCGCG1107.148199E-48.63640911
GTGTAAG5000.08.5522641
TACACTG9500.08.5004645
Produced by FastQC (version 0.11.5)