FastQCFastQC Report
Wed 25 May 2016
SRR1295248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361760
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA548852.3239025133798523No Hit
GCTTATGTACTCTGCGTTGATACCA340971.4437114694126414No Hit
CTTATGTACTCTGCGTTGATACCAC224070.9487416164216516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA201420.8528385610730981No Hit
GAGTACATAAGCAGTGGTATCAACG144800.6131020933541088No Hit
CCCATGTACTCTGCGTTGATACCAC135520.573809362509315No Hit
GTATCAACGCAGAGTACTTTTTTTT135250.5726661472799945No Hit
TATGTACTCTGCGTTGATACCACTG129430.5480235078924192No Hit
GAGTAAGCAGTGGTATCAACGCAGA128130.5425191382697649No Hit
GCGTTGATACCACTGCTTATGTACT108740.4604193482826367No Hit
CATAAGCAGTGGTATCAACGCAGAG95870.4059260890183592No Hit
GGTATCAACGCAGAGTACTTTTTTT95530.4044864846555111No Hit
GCTTACTCTGCGTTGATACCACTGC90230.3820455931169975No Hit
GTGGTATCAACGCAGAGTACATGGG88670.37544034956981237No Hit
TATCAACGCAGAGTACTTTTTTTTT85030.36002811462638035No Hit
ACTCTGCGTTGATACCACTGCTTAT81160.3436420296727864No Hit
CTGCTTATGTACTCTGCGTTGATAC79960.3385610730980286No Hit
ACGCAGAGTACATAAGCAGTGGTAT77580.32848384255809226No Hit
GTATCAACGCAGAGTACATAAGCAG72340.3062969988483165No Hit
CATGTACTCTGCGTTGATACCACTG67630.28635424429239215No Hit
TATCAACGCAGAGTACATAAGCAGT67230.2846605921008062No Hit
GGTATCAACGCAGAGTACATAAGCA63170.2674700223562089No Hit
GCGTTGATACCACTGCTTACTCTGC57450.24325079601653005No Hit
AAGCAGTGGTATCAACGCAGAGTAC52960.2242395501659779No Hit
GTACATGGGAAGCAGTGGTATCAAC45870.1942195650701172No Hit
GTACATGGGATAAGCAGTGGTATCA45740.19366912810785178No Hit
GCTTATCCCATGTACTCTGCGTTGA45730.19362678680306214No Hit
ACATAAGCAGTGGTATCAACGCAGA43570.18448106496849806No Hit
GTGGTATCAACGCAGAGTACATAAG42130.1783839170787887No Hit
GTACTCTGCGTTGATACCACTGCTT38390.1625482690874602No Hit
CTGCTTACTCTGCGTTGATACCACT37440.15852584513244358No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37110.1571285820743852No Hit
ACTCTGCGTTGATACCACTGCTTAC36020.15251337985231353No Hit
ACGCAGAGTAAGCAGTGGTATCAAC35740.15132782331820335No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33870.14340999932253912No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA33820.1431982927985909No Hit
GTATCAACGCAGAGTAAGCAGTGGT33690.14264785583632547No Hit
GCAGTGGTATCAACGCAGAGTACAT33650.14247849061716686No Hit
TGCTTACTCTGCGTTGATACCACTG33520.14192805365490144No Hit
TATCAACGCAGAGTAAGCAGTGGTA33090.14010737754894656No Hit
CCACTGCTTATGTACTCTGCGTTGA32330.13688943838493328No Hit
GGTATCAACGCAGAGTAAGCAGTGG30760.13024185353295847No Hit
GATACCACTGCTTATGTACTCTGCG29620.12541494478693857No Hit
GGATAAGCAGTGGTATCAACGCAGA27710.11732775557211572No Hit
GTTGATACCACTGCTTATGTACTCT27670.11715839035295712No Hit
GTGGTATCAACGCAGAGTAAGCAGT27230.11529537294221259No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN26940.11406747510331278No Hit
ATACCACTGCTTATGTACTCTGCGT26830.11360172075062665No Hit
GGGATAAGCAGTGGTATCAACGCAG26500.11220445769256826No Hit
GGTATCAACGCAGAGTACATGGGGG26430.1119080685590407No Hit
GTACTGGTTCACTATCGGTCAGTCA25420.10763159677528622No Hit
GGTATCAACGCAGAGTACATGGGAA24350.10310107716279386No Hit
TACCACTGCTTATGTACTCTGCGTT23920.10128040105683897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTC402.7684803E-416.61979316
ACTCGCG350.00217395716.28199815
CGGGATG1350.014.77471914
GACGTCC456.7802385E-414.773149516
CCGGGAT1300.014.6123613
TTGCGAA653.3811575E-614.6123611
TCTATCC400.00525990114.2582643
TAATACG602.5574103E-514.25705052
GCGTATC400.00526435614.2564441
TATCGAG400.005287424714.24705111
GTCATCG602.5769672E-514.24553716
TTAGCCC707.2181974E-613.5801654
TCTCATA1051.376975E-913.56862111
ATCGCCG1351.8189894E-1213.365046519
AATCGCC1351.8189894E-1213.365046518
TACGGAG500.00149316913.3079975
TAGGCTT1500.013.3079955
GCATACG500.001503250613.29611817
CTCGCGC500.001503491313.29583516
AATGCGC500.001504454613.29470419
Produced by FastQC (version 0.11.5)