FastQCFastQC Report
Wed 25 May 2016
SRR1295248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361760
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA596092.5239228372061513No Hit
GTATCAACGCAGAGTACTTTTTTTT421621.7851940925411556No Hit
GCTTATGTACTCTGCGTTGATACCA362191.5335597181762755No Hit
GGTATCAACGCAGAGTACTTTTTTT306771.298904207032044No Hit
TATCAACGCAGAGTACTTTTTTTTT259651.0993919788632205No Hit
CTTATGTACTCTGCGTTGATACCAC255521.081905019985096No Hit
CCCATGTACTCTGCGTTGATACCAC158270.6701358309057652No Hit
GAGTACATAAGCAGTGGTATCAACG154860.6556974459724951No Hit
GAGTAAGCAGTGGTATCAACGCAGA146920.6220784499695143No Hit
ACGCAGAGTACTTTTTTTTTTTTTT133920.5670347537429714No Hit
GTACTTTTTTTTTTTTTTTTTTTTT128200.5428155274032924No Hit
CATAAGCAGTGGTATCAACGCAGAG118070.4999237856513786No Hit
GCGTTGATACCACTGCTTATGTACT114360.48421516157441913No Hit
TATGTACTCTGCGTTGATACCACTG112930.4781603549894994No Hit
GCTTACTCTGCGTTGATACCACTGC96690.4093980760111104No Hit
GTGGTATCAACGCAGAGTACATGGG94620.4006334259196532No Hit
CTGCTTATGTACTCTGCGTTGATAC90130.38162218006910104No Hit
ACGCAGAGTACATAAGCAGTGGTAT89370.37840424090508773No Hit
ACTCTGCGTTGATACCACTGCTTAT88410.3743394756452815No Hit
GTATCAACGCAGAGTACATAAGCAG79190.3353007926292257No Hit
CATGTACTCTGCGTTGATACCACTG75400.3192534381139489No Hit
GAGTACTTTTTTTTTTTTTTTTTTT70550.2987179052909694No Hit
TATCAACGCAGAGTACATAAGCAGT70460.2983368335478626No Hit
GGTATCAACGCAGAGTACATAAGCA68300.2891911117132986No Hit
GCGTTGATACCACTGCTTACTCTGC62180.26327823318203375No Hit
AAGCAGTGGTATCAACGCAGAGTAC61560.26065307228507556No Hit
GCAGAGTACTTTTTTTTTTTTTTTT51830.21945498272474764No Hit
ACATAAGCAGTGGTATCAACGCAGA51780.2192432762007994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49120.2079804891267529No Hit
GTACATGGGATAAGCAGTGGTATCA46790.19811496511076485No Hit
GTACATGGGAAGCAGTGGTATCAAC45790.1938808346318No Hit
GTGGTATCAACGCAGAGTACTTTTT45510.19269527809768985No Hit
GTGGTATCAACGCAGAGTACATAAG45350.1920178172210555No Hit
GCTTATCCCATGTACTCTGCGTTGA45340.19197547591626585No Hit
CTGCTTACTCTGCGTTGATACCACT44020.18638642368403224No Hit
ACGCAGAGTAAGCAGTGGTATCAAC42800.18122078449969514No Hit
GCAGTGGTATCAACGCAGAGTACAT38930.16483469954610122No Hit
GTACTCTGCGTTGATACCACTGCTT38660.1636914843167807No Hit
ACTCTGCGTTGATACCACTGCTTAC38540.16318338865930493No Hit
GTATCAACGCAGAGTAAGCAGTGGT37030.156789851636068No Hit
TATCAACGCAGAGTAAGCAGTGGTA36860.156070049454644No Hit
CCACTGCTTATGTACTCTGCGTTGA36200.1532755233385272No Hit
GGTATCAACGCAGAGTAAGCAGTGG35510.15035397330804146No Hit
ATCAACGCAGAGTACTTTTTTTTTT34730.14705135153444887No Hit
GATACCACTGCTTATGTACTCTGCG33260.14082717973037057No Hit
GGATAAGCAGTGGTATCAACGCAGA32360.1370164622993022No Hit
TGCTTACTCTGCGTTGATACCACTG30150.12765903394078992No Hit
GGTATCAACGCAGAGTACATGGGAA29750.125965381749204No Hit
GGTATCAACGCAGAGTACATGGGGG29680.12566899261567643No Hit
GTGGTATCAACGCAGAGTAAGCAGT29260.12389065781451122No Hit
GGGATAAGCAGTGGTATCAACGCAG28990.1227474425851907No Hit
ATACCACTGCTTATGTACTCTGCGT28160.11923311428764989No Hit
GTTGATACCACTGCTTATGTACTCT27420.11609985773321589No Hit
GTAAGCAGTGGTATCAACGCAGAGT27000.11432152293205068No Hit
CCACTGCTTACTCTGCGTTGATACC26610.11267021204525438No Hit
CAGTGGTATCAACGCAGAGTACATA26570.11250084682609578No Hit
GTACTGGTTCACTATCGGTCAGTCA26400.11178104464467176No Hit
GAGTACATGGGATAAGCAGTGGTAT23860.10102635322810107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATG307.7203615E-419.0014617
CTCGCAT802.1827873E-1117.8138711
GGATAGA402.7589226E-416.6273351
CTGTACC704.4413173E-714.9278234
CGGACAA400.00527748514.25109612
AAGCGCG400.00527748514.2510967
GTGTGCG400.00527748514.2510969
GATTGGC400.00527748514.2510967
GCGGACA400.00527748514.25109611
AAGTATG551.9595525E-413.8192447
GCGCGTC551.9595525E-413.8192449
TCTCGCA1101.8553692E-1013.81924410
TCTATCC707.26854E-613.5710353
ATCTTAC500.001499080813.3010237
CGAAACG957.385643E-813.0017
CTATAGT1102.750312E-912.9538964
CGGGACT1102.750312E-912.9538964
CTCTCGC1251.0186341E-1012.9209949
GTTGGCG1251.03682396E-1012.91853215
GATCAGA751.4772189E-512.6684471
Produced by FastQC (version 0.11.5)