FastQCFastQC Report
Wed 25 May 2016
SRR1295247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767659
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA122341.593676358904149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA80041.0426504476597032No Hit
GCTTATGTACTCTGCGTTGATACCA74320.9681381967774754No Hit
GTATCAACGCAGAGTACTTTTTTTT52910.6892383206606058No Hit
CTTATGTACTCTGCGTTGATACCAC49970.6509400658365238No Hit
CCCATGTACTCTGCGTTGATACCAC35890.4675252944341173No Hit
GGTATCAACGCAGAGTACTTTTTTT35360.4606211872719528No Hit
GAGTACATAAGCAGTGGTATCAACG34920.45488947566562754No Hit
GTGGTATCAACGCAGAGTACATGGG33080.43092049985735853No Hit
TATGTACTCTGCGTTGATACCACTG32320.4210202707191604No Hit
TATCAACGCAGAGTACTTTTTTTTT31430.409426581333639No Hit
GCGTTGATACCACTGCTTATGTACT26040.3392131141561553No Hit
CATAAGCAGTGGTATCAACGCAGAG22530.29348968747842463No Hit
GTACATGGGAAGCAGTGGTATCAAC21010.2736892292020285No Hit
CTGCTTATGTACTCTGCGTTGATAC20950.27290763216480235No Hit
CATGTACTCTGCGTTGATACCACTG20770.27056284105312384No Hit
ACGCAGAGTACATAAGCAGTGGTAT20360.2652219279654117No Hit
ACTCTGCGTTGATACCACTGCTTAT18690.24346747709595015No Hit
GTACTGGTTCACTATCGGTCAGTCA17500.2279658025242979No Hit
GTATCAACGCAGAGTACATAAGCAG16780.2185866380775839No Hit
TATCAACGCAGAGTACATAAGCAGT16060.20920747363086994No Hit
ATACAGGGTGACAGCCCCGTACACA15390.20047964004851113No Hit
GGTATCAACGCAGAGTACATAAGCA15150.19735325189960645No Hit
GGATACCACGTGTCCCGCCCTACTC13460.17533826868440283No Hit
AAGCAGTGGTATCAACGCAGAGTAC13400.17455667164717667No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13060.17012762176956175No Hit
GTGGTATCAACGCAGAGTACATAAG11790.15358381781494126No Hit
GTACTCTGCGTTGATACCACTGCTT10840.14120853139219366No Hit
GGTATCAACGCAGAGTACATGGGAA10600.138082143243289No Hit
ACATAAGCAGTGGTATCAACGCAGA10380.13521628744012643No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10040.13078723756251148No Hit
GAGTACATGGGAAGCAGTGGTATCA9680.12609765533915449No Hit
GTATCAACGCAGAGTACATGGGAAG9440.12297126719024985No Hit
ACCCTGTATCGCGCGCCTTTCCAGA9190.11971461286847415No Hit
GCAGTGGTATCAACGCAGAGTACAT9090.11841195113976388No Hit
ATCATTAACTGAATCCATAGGTTAA8640.11254997336056766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN8330.10851172200156581No Hit
GTACATGATAAGCAGTGGTATCAAC8310.10825118965582374No Hit
ATTCCATTCCATTCCATTCCATTCC8310.10825118965582374No Hit
CCCATATTCAGACAGGATACCACGT8250.10746959261859759No Hit
GATACCACTGCTTATGTACTCTGCG8220.1070787940999845No Hit
CCACTGCTTATGTACTCTGCGTTGA8140.10603666471701628No Hit
ATACCACTGCTTATGTACTCTGCGT7950.10356160743246676No Hit
GGTTAATGAGGCGAACCGGGGGAAC7870.10251947804949854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATT250.00603377418.99758116
TTAGACA350.002164061616.2910964
TTAACTA350.002164061616.2910964
CTAAGAC350.002165007416.2900313
CCTAAGA350.002166900316.28792
GCGCATA350.00216974216.28470611
ACTAGAA602.5616513E-514.2519132
CGCATTT400.00527802814.24911817
CGCATAC400.00527802814.24911812
GTACAAA1101.8553692E-1013.8191321
CTGGACA500.001494605913.3043964
CCCACCG500.001499032613.29917513
GCATATA500.0015019913.29569919
TCTTACA655.4385713E-513.1556122
AAGACAC1051.9783329E-812.6708545
CTAGTCT604.0764222E-412.6708534
TGAGGTG604.0764222E-412.6708535
CTATACA604.0831766E-412.6683672
GCTATAC604.08543E-412.6675391
GGTTATA604.08543E-412.6675391
Produced by FastQC (version 0.11.5)