##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295247_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767659
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.482521536255028	31.0	30.0	33.0	23.0	34.0
2	29.56035557454547	31.0	30.0	34.0	23.0	34.0
3	29.712908986933	31.0	30.0	34.0	23.0	34.0
4	32.755902034627354	35.0	33.0	37.0	19.0	37.0
5	33.288092759936376	35.0	33.0	37.0	26.0	37.0
6	33.35169000819374	37.0	35.0	37.0	26.0	37.0
7	33.42620746972288	37.0	35.0	37.0	26.0	37.0
8	33.35552243899961	37.0	35.0	37.0	26.0	37.0
9	34.178165044635705	38.0	34.0	39.0	19.0	39.0
10	34.28092551510501	38.0	34.0	39.0	22.0	39.0
11	34.63376186562002	39.0	35.0	39.0	25.0	39.0
12	34.66847128738151	39.0	35.0	39.0	25.0	39.0
13	34.48128270495103	39.0	35.0	39.0	23.0	39.0
14	35.072659865904	39.0	34.0	41.0	19.0	41.0
15	35.397688296496234	39.0	34.0	41.0	23.0	41.0
16	35.3701500275513	39.0	34.0	41.0	24.0	41.0
17	35.152450502110966	39.0	34.0	41.0	22.0	41.0
18	35.07970205520941	39.0	34.0	41.0	21.0	41.0
19	35.499393610965285	39.0	34.0	41.0	24.0	41.0
20	35.523572315311874	39.0	34.0	41.0	24.0	41.0
21	35.57651639595185	39.0	34.0	41.0	24.0	41.0
22	35.51906640839227	39.0	34.0	41.0	24.0	41.0
23	35.29196036260892	39.0	34.0	41.0	23.0	41.0
24	35.59659953182337	39.0	34.0	41.0	25.0	41.0
25	34.46169458053641	38.0	32.0	41.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.16099621658796437
1101	2	0.28760927645146595
1101	3	0.005236180226699361
1101	4	-0.0026989615221850727
1101	5	-0.28634777158956126
1101	6	-0.3378945614283211
1101	7	-0.004163766063975061
1101	8	-0.12133281744270619
1101	9	0.11996971577791271
1101	10	0.21841325049802407
1101	11	0.16651994000964265
1101	12	0.3206216813229048
1101	13	0.19006604265445048
1101	14	0.42811429561484005
1101	15	0.11854550763945326
1101	16	-0.06550533171919426
1101	17	0.1133862794839402
1101	18	0.43469095194618035
1101	19	0.3011758748319764
1101	20	0.2808255146577636
1101	21	0.08777085392750905
1101	22	0.12814223823854576
1101	23	0.1352365734033043
1101	24	0.10695967338936185
1101	25	0.14852230647380082
1102	1	0.12604217288544106
1102	2	0.16980386708991801
1102	3	0.09670703592104246
1102	4	0.08317123704513563
1102	5	-0.2905773200032087
1102	6	-0.06262681829210948
1102	7	-0.16286920699401009
1102	8	-0.08805162707533043
1102	9	0.10882023037558497
1102	10	0.3236666543185933
1102	11	0.34621920702403486
1102	12	0.03914468469492505
1102	13	0.06398529330348879
1102	14	0.23489633644655328
1102	15	0.14308961006582877
1102	16	0.07831580534298865
1102	17	-0.11882974945226721
1102	18	0.3222137055973633
1102	19	0.2099578811111158
1102	20	0.35208390347152374
1102	21	0.20870405596107844
1102	22	-0.06807315618686971
1102	23	0.15129487929090146
1102	24	0.073235581925573
1102	25	0.07525829815605789
1103	1	-0.35173728947749083
1103	2	-0.24000670990456285
1103	3	-0.4053644881265832
1103	4	-0.24281919588636924
1103	5	-0.3451795378798863
1103	6	-0.5093488906206289
1103	7	-0.24959165061884647
1103	8	-0.3085614169216129
1103	9	-0.0781723313214826
1103	10	0.12673572781051234
1103	11	-0.06928148606242956
1103	12	-0.27567437023218133
1103	13	-0.12081971424620264
1103	14	-0.1562535063713355
1103	15	-0.09889193650710837
1103	16	-0.147169355180786
1103	17	-0.09947747588113742
1103	18	0.13006786040317309
1103	19	0.1108171226765009
1103	20	-0.05926136497571122
1103	21	-0.25384452197401686
1103	22	-0.12361753847356027
1103	23	-2.3189116852280236
1103	24	-1.08500781518822
1103	25	-0.283944590529309
1104	1	0.014686704939464335
1104	2	0.23320259094558438
1104	3	0.2445038661431802
1104	4	-0.11462321821856136
1104	5	0.24690585737452153
1104	6	0.023258012056693644
1104	7	0.13421751065509824
1104	8	-0.08533784123325461
1104	9	-2.002828149111103
1104	10	-0.2321797017035081
1104	11	0.015477744999621734
1104	12	0.23474626166732548
1104	13	0.3890854373843027
1104	14	0.5944935434954317
1104	15	0.3201395950244219
1104	16	0.4962293251897236
1104	17	0.5719617450299168
1104	18	0.825918640760996
1104	19	0.37416092422452607
1104	20	0.27817836348513936
1104	21	0.49485488165496605
1104	22	0.29374859526581076
1104	23	0.5250199224359804
1104	24	0.20503469210780878
1104	25	0.095291864235314
1105	1	0.10052147588061544
1105	2	-0.0854479745134924
1105	3	-0.12326304970884294
1105	4	0.20233288682265282
1105	5	0.07667392331280354
1105	6	0.004871966436603259
1105	7	-0.13388293021129982
1105	8	-0.2278526965140628
1105	9	-2.103660044836616
1105	10	-0.545950067814303
1105	11	-0.445131800716311
1105	12	-0.22235552096637434
1105	13	-0.24549382272687126
1105	14	-0.10986528341658897
1105	15	-0.21139193650710553
1105	16	0.25558911050695343
1105	17	-1.4811339038104165
1105	18	-0.1521377134655708
1105	19	-0.11979345568730082
1105	20	-0.18465468232662374
1105	21	-0.0028526130738057986
1105	22	-0.13673635777233528
1105	23	-0.07818498459871392
1105	24	0.006974504260384151
1105	25	-0.8221817796213102
1106	1	-0.31160484313426196
1106	2	-0.48578119932533426
1106	3	-0.2892235606535536
1106	4	-0.33122055362778013
1106	5	-0.309820896381936
1106	6	-0.232680554772962
1106	7	-0.20110088604943144
1106	8	-0.49420656594355705
1106	9	-2.8886756527803925
1106	10	-0.7480099333753216
1106	11	-0.451367317885051
1106	12	-0.2736951230116844
1106	13	-0.5315768991196208
1106	14	-0.19640338403515756
1106	15	-0.3294837456880231
1106	16	-0.41902030788168787
1106	17	-0.07581762750193377
1106	18	0.0798656570842482
1106	19	-0.29447296619154173
1106	20	-0.33188642677766467
1106	21	-0.2621735238882934
1106	22	-0.0508172126663311
1106	23	-0.22907224353737377
1106	24	-0.13047345330132742
1106	25	-0.6927304515097745
1107	1	-0.36500617658981227
1107	2	-0.2280722063893137
1107	3	-0.1866005270647193
1107	4	-1.868620119961708
1107	5	-0.6145810051140117
1107	6	-0.30278689762761246
1107	7	-0.2801472714893123
1107	8	-0.1773891579924154
1107	9	-0.055865493634257746
1107	10	0.3891140461344804
1107	11	-0.05673269582517548
1107	12	-0.603565338747778
1107	13	-0.2438989660508568
1107	14	0.037389360848678166
1107	15	-0.6050480299962544
1107	16	-0.9153951540474452
1107	17	-0.1918894183204145
1107	18	0.016980320136532612
1107	19	-0.00897613604107761
1107	20	-0.4879438522197077
1107	21	-0.16136644818907087
1107	22	-0.16076718930005285
1107	23	-0.05100919516242186
1107	24	-0.21980701103105815
1107	25	-0.3023423308947031
1108	1	0.11223827110416806
1108	2	0.06589367619141484
1108	3	0.16059952384467735
1108	4	0.44038434408827243
1108	5	0.2968806496850789
1108	6	0.29594822051332415
1108	7	0.2970197289317724
1108	8	0.12541817025268642
1108	9	0.5124038712304042
1108	10	0.7128741112335177
1108	11	0.42360558086468103
1108	12	0.3093281428185506
1108	13	0.3191176593873237
1108	14	0.49600381288891526
1108	15	0.33232258093003963
1108	16	0.27594699906798326
1108	17	0.46247707788101167
1108	18	0.5044438813300332
1108	19	0.39863211073951277
1108	20	0.3696757002379556
1108	21	0.5117221138506665
1108	22	0.41949770203681425
1108	23	0.5834491545878393
1108	24	0.31274386113084773
1108	25	0.6057431914749642
1109	1	0.029009428309034746
1109	2	0.09891596182858464
1109	3	0.050392468800765755
1109	4	0.2966256720287106
1109	5	0.06677807207710629
1109	6	-0.12739765168458916
1109	7	-0.07253415215472359
1109	8	0.08154131579129853
1109	9	0.4078327541184592
1109	10	0.0649240256102317
1109	11	-0.2438943896552317
1109	12	0.061537511721901694
1109	13	-0.1089352003778572
1109	14	0.4580878324532094
1109	15	-0.013168996280938927
1109	16	0.003644656514588007
1109	17	0.20410309478030797
1109	18	0.08060143340824766
1109	19	-0.1671058676076882
1109	20	0.03577634919298589
1109	21	0.008994222934859408
1109	22	0.2129615076935849
1109	23	0.3118222336324763
1109	24	0.06776301008653007
1109	25	0.2583956788738604
1110	1	0.2506331486207145
1110	2	0.30724711692381135
1110	3	0.455653136960958
1110	4	0.44226437181819733
1110	5	0.4740743834380723
1110	6	0.26588121932078934
1110	7	0.19374238675456468
1110	8	0.41604728501375376
1110	9	0.6887878552120839
1110	10	0.6946396985965535
1110	11	0.3087817830550392
1110	12	0.0509219480087566
1110	13	0.14648925003672986
1110	14	0.2923795905527484
1110	15	0.20818185986226467
1110	16	0.22610482025859113
1110	17	0.6238854919812837
1110	18	0.8687096559277023
1110	19	0.22593392848371963
1110	20	0.214428425702053
1110	21	0.37078724653760986
1110	22	0.20647142069582003
1110	23	0.6765319711855966
1110	24	0.5501970604745949
1110	25	0.4967374297692757
1111	1	-0.06965812313100983
1111	2	-0.20860679443356034
1111	3	-0.12261430424856812
1111	4	-0.008354829798967955
1111	5	0.04226653371726741
1111	6	-0.1841935548648621
1111	7	-0.13172309869938204
1111	8	-0.22937174263270066
1111	9	0.03259839200522663
1111	10	-0.06391627069650951
1111	11	-0.24486646514973387
1111	12	-0.3981138002652216
1111	13	-0.17526613164380933
1111	14	-0.4053210801695428
1111	15	-0.4620544462499012
1111	16	-0.35780436555479866
1111	17	-0.17327499599794294
1111	18	0.02730475017516909
1111	19	-0.12189491337456815
1111	20	-0.6409821064157768
1111	21	-0.8123396862566707
1111	22	-0.4346863088510844
1111	23	-0.14900702735090476
1111	24	-0.22884845058281655
1111	25	-0.057672401667375084
1112	1	-0.09234004867302659
1112	2	-0.15187034238228136
1112	3	0.01991843093558998
1112	4	0.2601529236289011
1112	5	0.17340656141695376
1112	6	-0.02099959912049343
1112	7	0.005949181628047029
1112	8	0.11446803444516007
1112	9	-1.6156717970078631
1112	10	-0.18072035160986388
1112	11	-0.32692884975902103
1112	12	-0.2346791576822227
1112	13	-0.025192269761390662
1112	14	0.04381889312426779
1112	15	0.07267483132293506
1112	16	-0.15263805508888595
1112	17	-0.04491203314887571
1112	18	-0.0048035837396795955
1112	19	-0.0903826785588322
1112	20	-0.09712683549228984
1112	21	-0.15475071787476224
1112	22	0.10632566398855658
1112	23	-0.22281805210912609
1112	24	-0.05968936427026961
1112	25	0.053940649591233125
1113	1	0.11085988108296618
1113	2	0.011944537640051323
1113	3	0.035943970936401115
1113	4	0.20716147448601419
1113	5	0.28950617282350066
1113	6	0.00859631772141256
1113	7	0.008683519690841024
1113	8	-0.004663101211328069
1113	9	-0.3403839541743281
1113	10	0.17301451328299322
1113	11	0.029396262924073824
1113	12	-0.21484873392173398
1113	13	-0.011280804228228192
1113	14	-0.4198861180652358
1113	15	-0.19625840534983752
1113	16	-0.21332369964520126
1113	17	0.2199393962716627
1113	18	-0.06045957721959638
1113	19	-0.028536431885292757
1113	20	0.18907471978209145
1113	21	0.017898696983650098
1113	22	0.1027977037214498
1113	23	0.08396477233038269
1113	24	-0.060835332219191685
1113	25	0.07282548093807151
1114	1	0.2875756288487956
1114	2	0.28040401977966667
1114	3	0.3250819661090354
1114	4	0.4308683374770723
1114	5	0.5274182940094079
1114	6	0.42186594944013933
1114	7	0.17565369903006456
1114	8	0.34882678987015225
1114	9	-0.09267003181012967
1114	10	0.24967489341639038
1114	11	0.3761230842163883
1114	12	0.2842104488866113
1114	13	0.34153861942932195
1114	14	0.6368700307698774
1114	15	0.492398150956447
1114	16	0.40753129771619
1114	17	0.7826648679779353
1114	18	0.5470596067999196
1114	19	0.3498970101646961
1114	20	0.2928436118238622
1114	21	0.43192314525384035
1114	22	0.19906601180771588
1114	23	0.8117139050483289
1114	24	0.5159807693804197
1114	25	0.5186798294600123
1115	1	0.40939895203615606
1115	2	0.34243170266919165
1115	3	0.33789615792138505
1115	4	0.5186645082459833
1115	5	0.4124039306495604
1115	6	0.4458697654094621
1115	7	0.3783107601775484
1115	8	0.21396681925996575
1115	9	0.38772660226536004
1115	10	0.15145272015341504
1115	11	0.23982784711935068
1115	12	0.38498123618696667
1115	13	0.06734550229146663
1115	14	0.34087572905590235
1115	15	0.3120123188120374
1115	16	0.20927949935508394
1115	17	0.40705391717271056
1115	18	0.8143186328293694
1115	19	0.49916472479692686
1115	20	0.49766373293383026
1115	21	0.511131246060458
1115	22	0.16492174934432313
1115	23	0.5701495715274802
1115	24	0.5743406084423412
1115	25	0.44842717122241993
1116	1	0.6531162784243882
1116	2	0.589813075969353
1116	3	0.6910448720937801
1116	4	0.797028655056927
1116	5	0.8238514376411317
1116	6	0.6865803750100525
1116	7	0.4353311704403495
1116	8	0.5995163856873944
1116	9	0.8597096654562293
1116	10	0.7468535751157432
1116	11	0.4421257952639053
1116	12	0.44123873199743713
1116	13	0.23433659259855943
1116	14	0.910959086529239
1116	15	0.7376855542677987
1116	16	0.695953403511588
1116	17	1.1687836591950003
1116	18	1.1396515618739897
1116	19	0.8510599634851914
1116	20	1.1995174147134904
1116	21	0.8525324854580276
1116	22	0.9202503737971099
1116	23	1.1507637474418004
1116	24	0.927809865805493
1116	25	0.7552665991178031
1201	1	-0.29326590959678
1201	2	-0.10049302637992596
1201	3	-0.31029719826642577
1201	4	-0.36903154113222314
1201	5	-0.6866599148033501
1201	6	-0.5549167819543968
1201	7	-0.31644293626869313
1201	8	-0.3011244463436995
1201	9	-0.2710417363638413
1201	10	-0.16565249834356166
1201	11	-0.03372875687592369
1201	12	-0.20235097792101442
1201	13	-0.3128558277242206
1201	14	0.001423427115703646
1201	15	-0.2005378439804204
1201	16	-0.055187750248705925
1201	17	-0.015725408315603318
1201	18	0.12475484351178778
1201	19	-0.005671399202100247
1201	20	0.05491524271312187
1201	21	0.027043654057258948
1201	22	-0.3062305653895052
1201	23	-0.3585650860600609
1201	24	-0.3496749997904942
1201	25	-0.01938704955392012
1202	1	-0.5023343160815585
1202	2	-0.41556617782318384
1202	3	-0.4288895272502131
1202	4	-0.5507073023026479
1202	5	-0.6338508993822387
1202	6	-0.6071564023577167
1202	7	-0.3689656725280841
1202	8	-0.5411576610530702
1202	9	-0.05584917013212731
1202	10	0.0715440220202126
1202	11	-0.3062760087541321
1202	12	-0.5014098944888303
1202	13	-0.21077041847155442
1202	14	-0.425326276324391
1202	15	-0.2656141587293277
1202	16	-0.08033523937483977
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2211	8	-0.12137530500447014
2211	9	-1.683366690822389
2211	10	-0.4415956152398621
2211	11	-0.34114623238505715
2211	12	-0.053299311639740665
2211	13	-0.353458215618943
2211	14	-0.21402146914002174
2211	15	-0.29332537549284865
2211	16	-0.03787212967242937
2211	17	-0.12122224720320673
2211	18	0.06485726242544843
2211	19	-0.1278536101196437
2211	20	-0.10285274686450663
2211	21	-0.24912364520787378
2211	22	-0.323142939692282
2211	23	-2.520187884420004
2211	24	-0.9827082013403157
2211	25	-0.7263440347381405
2212	1	-0.04109830134670034
2212	2	-1.3642648842397023
2212	3	-0.16999806832791364
2212	4	0.42461146750802925
2212	5	0.1236366359557266
2212	6	0.4580854377862025
2212	7	0.33950344423710277
2212	8	0.3423108560376491
2212	9	0.7673955827163255
2212	10	0.6102709988042463
2212	11	0.3282535894639764
2212	12	0.2530525401894863
2212	13	0.11996820913060446
2212	14	0.5194180053512696
2212	15	0.426695958635527
2212	16	0.7309879851604109
2212	17	0.8289400803428677
2212	18	1.0134824574689958
2212	19	0.7963882402066673
2212	20	0.7737599898300687
2212	21	0.4594266237903142
2212	22	0.6515291977649937
2212	23	0.6091169724543875
2212	24	0.6305044178088366
2212	25	0.5832238208642622
2213	1	0.3108145308786092
2213	2	0.27768329806779946
2213	3	0.271068544028612
2213	4	0.629133368556893
2213	5	0.511406962252984
2213	6	0.4453215850636667
2213	7	0.22868631908617232
2213	8	0.3369807037268018
2213	9	0.5270103686519434
2213	10	0.518168759965711
2213	11	0.48117891787060074
2213	12	0.3322169398927173
2213	13	0.40568241171712316
2213	14	-1.3802948297835087
2213	15	-0.13667495537503527
2213	16	0.27501905831908147
2213	17	0.5615316923357128
2213	18	0.36558207778292484
2213	19	0.404172822915676
2213	20	0.48487562678617735
2213	21	0.3585604740398054
2213	22	0.3717020203890513
2213	23	0.902208431699961
2213	24	0.6622775953884457
2213	25	0.33457454314063284
2214	1	0.24836653861929747
2214	2	0.32059987834287185
2214	3	0.291647713287027
2214	4	0.6039789003835594
2214	5	0.481724192192857
2214	6	0.4813710701697502
2214	7	-0.011847235409646828
2214	8	0.09374343784802619
2214	9	0.17444142815847385
2214	10	0.1529603834365787
2214	11	0.04511837464024637
2214	12	0.08017740709846777
2214	13	-0.04259459429572843
2214	14	0.18419753319942345
2214	15	0.2083882832731092
2214	16	0.23190896086936164
2214	17	0.21362098668915763
2214	18	0.3368779517203109
2214	19	-0.05684590678229995
2214	20	0.38557367088044714
2214	21	0.4502157370158244
2214	22	0.3749088764260904
2214	23	0.41287261028866595
2214	24	0.22325071032382482
2214	25	0.11019137913483235
2215	1	0.49603680959619467
2215	2	0.40288166550448423
2215	3	0.3476225711555472
2215	4	0.6820080816902347
2215	5	0.591654320331287
2215	6	0.5924804026920469
2215	7	0.45272567650485485
2215	8	0.3912977892989531
2215	9	0.5736906858199617
2215	10	0.634547987383975
2215	11	0.35643953709032417
2215	12	0.21871311271356575
2215	13	0.14357189762458233
2215	14	0.3734112813352368
2215	15	0.1253888355249373
2215	16	0.30064590491584653
2215	17	0.5977460143684468
2215	18	0.7323574903899441
2215	19	0.42139883948866697
2215	20	-0.7106499404196356
2215	21	0.14714847167616085
2215	22	-0.7038603756862045
2215	23	0.3899490904969767
2215	24	0.07529577620720573
2215	25	0.29878457763334865
2216	1	0.02430516643707037
2216	2	-0.5747375525877807
2216	3	-8.014244089800115E-4
2216	4	0.06887641996051741
2216	5	0.18037263291940775
2216	6	0.015967154507912085
2216	7	-0.0301660327999258
2216	8	0.14345447355758978
2216	9	0.2642209191170366
2216	10	0.026048238838356497
2216	11	0.04700197372355319
2216	12	-0.18084656033669688
2216	13	-0.230219202554089
2216	14	-0.22979692235730198
2216	15	-0.15199621064122937
2216	16	-0.10219260447760092
2216	17	0.10794400584637032
2216	18	0.5122696933611266
2216	19	0.16597646858244275
2216	20	-1.1714528447311778
2216	21	-1.1473440601018225
2216	22	-1.2255699863961738
2216	23	-1.4246557665175246
2216	24	-1.267433059357728
2216	25	-0.9236615770467935
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1480.0
3	203.0
4	189.0
5	126.0
6	159.0
7	220.0
8	123.0
9	249.0
10	593.0
11	1274.0
12	2206.0
13	3760.0
14	5518.0
15	7344.0
16	8961.0
17	9841.0
18	9763.0
19	9178.0
20	8614.0
21	7995.0
22	7286.0
23	6837.0
24	6618.0
25	6546.0
26	6822.0
27	7005.0
28	7896.0
29	9442.0
30	11751.0
31	14857.0
32	19846.0
33	26598.0
34	37007.0
35	53562.0
36	81693.0
37	134622.0
38	251475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.263556690071496	21.738574657829965	13.51827624303627	20.47959240906227
2	14.641206198184642	24.679752187560002	38.078369443257984	22.600672170997377
3	18.856755990228997	31.02764137959878	29.77176159065037	20.343841039521855
4	12.58840816924167	21.69317495470103	34.58462214250416	31.133794733553138
5	11.980315429037619	41.766041761727266	31.764512076018843	14.489130733216276
6	27.18199925763667	38.80991839231288	19.494748563631518	14.513333786418933
7	21.99724750138213	39.117109275240715	21.178555604785114	17.707087618592045
8	22.343761642141644	37.84844725693169	21.752520584940413	18.055270515986255
9	25.281796176146138	21.425920020076017	19.61280473459401	33.679479069183834
10	16.28944426648537	31.4482019232655	28.06477129353006	24.197582516719073
11	28.10544866895812	30.671510856969665	20.854970847328314	20.3680696267439
12	22.409065363150397	31.29079478767759	26.123433231822872	20.17670661734914
13	26.40539802460941	26.73558799817592	26.731145394653456	20.127868582561213
14	23.43015749389731	27.51020600180859	25.16648458776116	23.89315191653294
15	26.74105923124488	29.319917223456144	23.396662008416225	20.542361536882755
16	21.74256737510812	31.866015454528824	25.633115652871524	20.758301517491525
17	19.213186589085165	33.40375105070991	26.150944037523583	21.232118322681345
18	19.305098523004382	29.62812433840999	31.577674914597715	19.489102223987913
19	21.361913475362698	31.90132008887727	26.596784734021696	20.139981701738336
20	22.16021929091061	28.92382306212894	28.53212016054854	20.383837486411906
21	23.598278343225584	30.810362878507654	25.481487484266292	20.109871294000474
22	21.95476619445232	29.603828581358005	26.311567400617452	22.129837823572217
23	23.0175991347386	28.657473264284842	26.323984486949886	22.000943114026665
24	21.223180929009093	28.915790175193756	25.954340119200225	23.906688776596926
25	22.589235778986925	30.133572724089085	26.105614183640935	21.171577313283052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10321.0
1	10321.0
2	7355.0
3	4389.0
4	4389.0
5	4389.0
6	5014.0
7	5639.0
8	5639.0
9	5639.0
10	6248.5
11	6858.0
12	6858.0
13	6858.0
14	8524.5
15	10191.0
16	10191.0
17	10191.0
18	13976.0
19	17761.0
20	17761.0
21	17761.0
22	22560.5
23	27360.0
24	27360.0
25	27360.0
26	33478.0
27	39596.0
28	39596.0
29	39596.0
30	44035.0
31	48474.0
32	48474.0
33	48474.0
34	52973.0
35	57472.0
36	57472.0
37	57472.0
38	62837.0
39	68202.0
40	68202.0
41	68202.0
42	96203.0
43	124204.0
44	124204.0
45	124204.0
46	113496.0
47	102788.0
48	102788.0
49	102788.0
50	92525.0
51	82262.0
52	82262.0
53	82262.0
54	70049.5
55	57837.0
56	57837.0
57	57837.0
58	49404.0
59	40971.0
60	40971.0
61	40971.0
62	35890.0
63	30809.0
64	30809.0
65	30809.0
66	23600.0
67	16391.0
68	16391.0
69	16391.0
70	12949.0
71	9507.0
72	9507.0
73	9507.0
74	6954.0
75	4401.0
76	4401.0
77	4401.0
78	3071.0
79	1741.0
80	1741.0
81	1741.0
82	1044.5
83	348.0
84	348.0
85	348.0
86	214.5
87	81.0
88	81.0
89	81.0
90	59.5
91	38.0
92	38.0
93	38.0
94	25.5
95	13.0
96	13.0
97	13.0
98	9.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1313083022539956
2	0.2709536395717369
3	0.3306155467466675
4	0.35705957983948605
5	0.3642242193473925
6	0.3302247482280544
7	0.3303550144009254
8	0.3462474874911907
9	0.3343932657599273
10	0.36656901045907103
11	0.2873671773534864
12	0.33204847464824877
13	0.3050833768639461
14	0.3156349368664993
15	0.35276079613474215
16	0.3018267225421704
17	0.3517186667517739
18	0.3200639867441143
19	0.333351136376959
20	0.2973976726645555
21	0.3363472583529927
22	0.2941410183427798
23	0.27486162475786774
24	0.3115966855074975
25	0.3104242899516582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	767659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.677688208474297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90125681975805	20.248700640688387
2	7.782666037409514	4.152470759533864
3	3.4160471499342675	2.7339672231418093
4	2.1525809598595838	2.2970353476252923
5	1.5383866955532643	2.0520300304017534
6	1.165123674976303	1.8649683675197735
7	0.9235013715663104	1.724581715552108
8	0.7642898504904109	1.6311589065827696
9	0.6201669525363301	1.4890158537267506
>10	5.022107438485984	26.793522314357922
>50	0.4687243374007459	8.434508366172675
>100	0.20494432325643216	10.112672598184599
>500	0.025005168626980958	4.59683583556147
>1k	0.013238030449578154	7.557232644867833
>5k	0.0014708922721753503	2.7110919748373155
>10k+	4.902974240584501E-4	1.6002074212457043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	12234	1.593676358904149	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8004	1.0426504476597032	No Hit
GCTTATGTACTCTGCGTTGATACCA	7432	0.9681381967774754	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5291	0.6892383206606058	No Hit
CTTATGTACTCTGCGTTGATACCAC	4997	0.6509400658365238	No Hit
CCCATGTACTCTGCGTTGATACCAC	3589	0.4675252944341173	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3536	0.4606211872719528	No Hit
GAGTACATAAGCAGTGGTATCAACG	3492	0.45488947566562754	No Hit
GTGGTATCAACGCAGAGTACATGGG	3308	0.43092049985735853	No Hit
TATGTACTCTGCGTTGATACCACTG	3232	0.4210202707191604	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3143	0.409426581333639	No Hit
GCGTTGATACCACTGCTTATGTACT	2604	0.3392131141561553	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2253	0.29348968747842463	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2101	0.2736892292020285	No Hit
CTGCTTATGTACTCTGCGTTGATAC	2095	0.27290763216480235	No Hit
CATGTACTCTGCGTTGATACCACTG	2077	0.27056284105312384	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	2036	0.2652219279654117	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1869	0.24346747709595015	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1750	0.2279658025242979	No Hit
GTATCAACGCAGAGTACATAAGCAG	1678	0.2185866380775839	No Hit
TATCAACGCAGAGTACATAAGCAGT	1606	0.20920747363086994	No Hit
ATACAGGGTGACAGCCCCGTACACA	1539	0.20047964004851113	No Hit
GGTATCAACGCAGAGTACATAAGCA	1515	0.19735325189960645	No Hit
GGATACCACGTGTCCCGCCCTACTC	1346	0.17533826868440283	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1340	0.17455667164717667	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1306	0.17012762176956175	No Hit
GTGGTATCAACGCAGAGTACATAAG	1179	0.15358381781494126	No Hit
GTACTCTGCGTTGATACCACTGCTT	1084	0.14120853139219366	No Hit
GGTATCAACGCAGAGTACATGGGAA	1060	0.138082143243289	No Hit
ACATAAGCAGTGGTATCAACGCAGA	1038	0.13521628744012643	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1004	0.13078723756251148	No Hit
GAGTACATGGGAAGCAGTGGTATCA	968	0.12609765533915449	No Hit
GTATCAACGCAGAGTACATGGGAAG	944	0.12297126719024985	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	919	0.11971461286847415	No Hit
GCAGTGGTATCAACGCAGAGTACAT	909	0.11841195113976388	No Hit
ATCATTAACTGAATCCATAGGTTAA	864	0.11254997336056766	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	833	0.10851172200156581	No Hit
GTACATGATAAGCAGTGGTATCAAC	831	0.10825118965582374	No Hit
ATTCCATTCCATTCCATTCCATTCC	831	0.10825118965582374	No Hit
CCCATATTCAGACAGGATACCACGT	825	0.10746959261859759	No Hit
GATACCACTGCTTATGTACTCTGCG	822	0.1070787940999845	No Hit
CCACTGCTTATGTACTCTGCGTTGA	814	0.10603666471701628	No Hit
ATACCACTGCTTATGTACTCTGCGT	795	0.10356160743246676	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	787	0.10251947804949854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.907985186130821E-4	0.0
2	0.0	0.0	0.0	3.907985186130821E-4	0.0
3	0.0	0.0	0.0	3.907985186130821E-4	0.0
4	0.0	0.0	0.0	3.907985186130821E-4	0.0
5	3.907985186130821E-4	0.0	0.0	7.815970372261642E-4	0.0
6	5.210646914841095E-4	0.0	0.0	0.001042129382968219	0.0
7	5.210646914841095E-4	0.0	0.0	0.001042129382968219	0.0
8	7.815970372261642E-4	0.0	0.0	0.0011723955558392463	0.0
9	7.815970372261642E-4	0.0	0.0	0.0013026617287102736	0.0
10	7.815970372261642E-4	0.0	0.0	0.0013026617287102736	0.0
11	7.815970372261642E-4	0.0	0.0	0.0013026617287102736	0.0
12	7.815970372261642E-4	0.0	0.0	0.0013026617287102736	0.0
13	7.815970372261642E-4	0.0	0.0	0.0015631940744523284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATT	25	0.006033774	18.997581	16
TTAGACA	35	0.0021640616	16.291096	4
TTAACTA	35	0.0021640616	16.291096	4
CTAAGAC	35	0.0021650074	16.290031	3
CCTAAGA	35	0.0021669003	16.2879	2
GCGCATA	35	0.002169742	16.284706	11
ACTAGAA	60	2.5616513E-5	14.251913	2
CGCATTT	40	0.005278028	14.249118	17
CGCATAC	40	0.005278028	14.249118	12
GTACAAA	110	1.8553692E-10	13.819132	1
CTGGACA	50	0.0014946059	13.304396	4
CCCACCG	50	0.0014990326	13.299175	13
GCATATA	50	0.00150199	13.295699	19
TCTTACA	65	5.4385713E-5	13.155612	2
AAGACAC	105	1.9783329E-8	12.670854	5
CTAGTCT	60	4.0764222E-4	12.670853	4
TGAGGTG	60	4.0764222E-4	12.670853	5
CTATACA	60	4.0831766E-4	12.668367	2
GCTATAC	60	4.08543E-4	12.667539	1
GGTTATA	60	4.08543E-4	12.667539	1
>>END_MODULE