FastQCFastQC Report
Wed 25 May 2016
SRR1295247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767659
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT146971.9145219426854894No Hit
GTACATAAGCAGTGGTATCAACGCA126871.6526869352147242No Hit
GGTATCAACGCAGAGTACTTTTTTT102681.3375730630397091No Hit
TATCAACGCAGAGTACTTTTTTTTT84451.1000978298958262No Hit
GCTTATGTACTCTGCGTTGATACCA78171.018290673332821No Hit
CTTATGTACTCTGCGTTGATACCAC58260.7589307231466055No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47410.6175919255815407No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41100.5353939704999225No Hit
CCCATGTACTCTGCGTTGATACCAC39370.5128579225932348No Hit
GAGTACATAAGCAGTGGTATCAACG38070.4959233201200012No Hit
GTGGTATCAACGCAGAGTACATGGG35660.46452917245808356No Hit
TATGTACTCTGCGTTGATACCACTG29490.3841549437966597No Hit
GCGTTGATACCACTGCTTATGTACT28890.37633897342439804No Hit
CATAAGCAGTGGTATCAACGCAGAG27860.3629215576186823No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24200.3152441383478863No Hit
CATGTACTCTGCGTTGATACCACTG22440.29231729192258543No Hit
CTGCTTATGTACTCTGCGTTGATAC22430.2921870257497144No Hit
ACTCTGCGTTGATACCACTGCTTAT21970.2861947817976471No Hit
ACGCAGAGTACATAAGCAGTGGTAT21760.28345919216735554No Hit
GTACATGGGAAGCAGTGGTATCAAC21170.2757734879679649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20440.26626405734837993No Hit
GTATCAACGCAGAGTACATAAGCAG19030.2478965269735651No Hit
GTACTGGTTCACTATCGGTCAGTCA17990.2343488449949782No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17700.23057112598171844No Hit
ATACAGGGTGACAGCCCCGTACACA17590.22913819808013713No Hit
TATCAACGCAGAGTACATAAGCAGT17240.2245788820296512No Hit
GGTATCAACGCAGAGTACATAAGCA16170.21064040153245125No Hit
CTTATACACATCTCCGAGCCCACGA15330.19969804301128496No Hit
AAGCAGTGGTATCAACGCAGAGTAC15320.19956777683841392No Hit
GGATACCACGTGTCCCGCCCTACTC14320.18654115955131118No Hit
ATCAACGCAGAGTACTTTTTTTTTT13480.17559880103014489No Hit
GTGGTATCAACGCAGAGTACTTTTT13430.17494747016578976No Hit
GTGGTATCAACGCAGAGTACATAAG12410.16166032053294496No Hit
ACATAAGCAGTGGTATCAACGCAGA12260.15970632793987954No Hit
GGTATCAACGCAGAGTACATGGGAA11460.14928503411019736No Hit
GAGTACATGGGAAGCAGTGGTATCA10730.13977560349061238No Hit
GCAGTGGTATCAACGCAGAGTACAT10730.13977560349061238No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10710.1395150711448703No Hit
GTACTCTGCGTTGATACCACTGCTT10690.13925453879912825No Hit
CCCATATTCAGACAGGATACCACGT9530.12414366274608908No Hit
GATACCACTGCTTATGTACTCTGCG9460.12323179953599189No Hit
CCACTGCTTATGTACTCTGCGTTGA9280.12088700842431341No Hit
ATCATTAACTGAATCCATAGGTTAA8990.11710928941105361No Hit
GTATCAACGCAGAGTACATGGGAAG8840.1151552968179882No Hit
ATCATGTACTCTGCGTTGATACCAC8580.11176837632334148No Hit
GCGTTGATACCACTGCTTCCCATGT8250.10746959261859759No Hit
GGTATCAACGCAGAGTACATGGGGG8240.10733932644572655No Hit
ATACCACTGCTTATGTACTCTGCGT8110.1056458661984032No Hit
GGTTAATGAGGCGAACCGGGGGAAC8020.10447347064256395No Hit
TCTAAGTACCCCGAGGAAAAGAAAT8000.10421293829682189No Hit
GTACATGATAAGCAGTGGTATCAAC7890.10278001039524058No Hit
GGTATCAACGCAGAGTACATGGGGA7750.10095628397504622No Hit
GTACATGGGATAAGCAGTGGTATCA7730.10069575162930415No Hit
ATTCCATTCCATTCCATTCCATTCC7710.1004352192835621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT359.943329E-518.99700411
GAGAGCG453.5399094E-516.8862259
TTTATGG350.002166421216.288453
TCAGCTG551.1257262E-515.5470535
TTATACA1600.015.4400932
GAGTCCT456.7649013E-414.7754479
GTACCAG400.00525862414.2570381
GACACCC400.00527912514.2486817
TGTCTAA400.00528140714.24775210
CCGAGCT400.00528140714.2477529
TATACAC2100.014.0261663
CTTATAC1850.013.3579461
GAGAGTC500.00149938713.2987697
GTACAAA1301.4551915E-1113.1603441
GTCTTAC1450.013.1099211
AGGTTAC604.08878E-412.6663196
ACTTACT604.0932768E-412.664678
GTGTAAG853.914296E-612.3001911
TTTTGGG701.0878061E-412.2163382
TAAACTG701.08846805E-412.2155425
Produced by FastQC (version 0.11.5)