FastQCFastQC Report
Wed 25 May 2016
SRR1295246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5292717
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG71600.13528023508530684No Hit
GTATCAACGCAGAGTACTTTTTTTT64500.12186557490226665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN60590.11447806485780365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGC550.003072957512.0894186
GTGGTAT17350.011.2325621
TGGTATC17050.010.8153342
GGTATCA36300.010.7898411
CCGCGCG750.002652079810.1320849
TAGACAG8100.09.1534445
ATCGCTC950.00182669778.9990628
GCGTCAG2750.08.9880421
CTTACAC6900.08.6789353
CTAGGAC4650.08.5856123
GTCCTAA5100.08.5745651
CGTCAGA2803.6379788E-128.4868122
TTAGGCT4400.08.4254894
GTCTTAC5200.08.409671
TATACTG10350.08.3574935
CTAGACT6000.08.2382564
TGAACCG1401.0330498E-48.1475725
GCTAGAC2451.3842509E-98.1475723
GAACCGC1050.0045205618.1418536
AACCGCA1050.0045209478.1417767
Produced by FastQC (version 0.11.5)