FastQCFastQC Report
Wed 25 May 2016
SRR1295246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5292717
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT177400.33517756570018764No Hit
GGTATCAACGCAGAGTACTTTTTTT153590.2901912193680486No Hit
TATCAACGCAGAGTACTTTTTTTTT101440.1916595956292392No Hit
GTGGTATCAACGCAGAGTACATGGG76870.1452373138408874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67750.12800608836633434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA6000.013.1422493
GCAGCGT5450.013.0754561
CAGCGTC6350.012.4178722
GTGGTAT20500.012.3751221
GGTATCA53600.012.1250451
TGGTATC20500.011.6322222
CGCCAAC803.768414E-410.6891851
GGTCGGC803.7723643E-410.68797311
ATAACCG909.519815E-510.5560235
ACCGTAC856.595847E-410.0592688
GCGTCAG7950.09.9186784
TACACCG1106.839735E-59.5004215
TAGCCCG900.0011139959.500425
CACCGTT1054.4948535E-49.0478497
CGTCAGA8900.08.9666895
ACCGTCC850.0074369918.9415718
GCACCGT1502.7059214E-68.8664736
TACGACC1304.3707598E-58.7696194
GTGCTAG6000.08.5513481
GTCCTAT4350.08.5185841
Produced by FastQC (version 0.11.5)