FastQCFastQC Report
Wed 25 May 2016
SRR1295245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819542
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63920.13262671017287536No Hit
GTATCAACGCAGAGTACTTTTTTTT59050.1225220155774138No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54080.11220983238656287No Hit
ATTCCATTCCATTCCATTCCATTCC53040.11005195099451359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG250.00603729418.9981377
CGGCGTA400.005286497514.24785912
AGCCGCG1701.0913936E-1111.1822472
TACATCG909.4718955E-510.5612325
GGTATCA35550.010.3212411
CGCGATC1356.4761844E-79.8491417
GTGGTAT18350.09.8424181
GCGTCAG1856.4210326E-109.7626131
TGGTATC18000.09.5577152
TAGACAG7050.09.4376965
GCGCGAT1451.714825E-69.16988916
CTGTCGC3800.08.7491429
TATTCCG2106.330083E-98.5998595
ATACCGT1451.6933727E-58.5162286
GTCTAAT4800.08.5155261
GTATTAG7300.08.4639831
TCTGTCG3750.08.3592678
AGCCTCG3200.08.3167962
GTATCAA62600.08.2909161
GTCTTAG4950.08.257481
Produced by FastQC (version 0.11.5)