##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295245_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4819542
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.143616758604864	31.0	30.0	33.0	26.0	34.0
2	30.144903395384873	31.0	30.0	34.0	26.0	34.0
3	30.219115218832	31.0	30.0	34.0	26.0	34.0
4	33.611910426343414	35.0	35.0	37.0	28.0	37.0
5	33.74883173546366	35.0	35.0	37.0	28.0	37.0
6	34.07766962088929	37.0	35.0	37.0	29.0	37.0
7	33.88868921569726	36.0	35.0	37.0	28.0	37.0
8	33.96983904279701	37.0	35.0	37.0	28.0	37.0
9	35.1092109997174	39.0	35.0	39.0	27.0	39.0
10	35.099239305311585	39.0	35.0	39.0	27.0	39.0
11	35.54530305991731	39.0	35.0	39.0	28.0	39.0
12	35.549472335753066	39.0	35.0	39.0	28.0	39.0
13	35.483962376507975	39.0	35.0	39.0	28.0	39.0
14	36.140066836226346	39.0	36.0	41.0	27.0	41.0
15	36.513313298234564	40.0	36.0	41.0	27.0	41.0
16	36.47002764993022	40.0	36.0	41.0	27.0	41.0
17	36.12283075860735	39.0	36.0	41.0	27.0	41.0
18	36.024462075442024	39.0	36.0	41.0	27.0	41.0
19	36.46005201324109	40.0	36.0	41.0	27.0	41.0
20	36.51639574880767	40.0	36.0	41.0	27.0	41.0
21	36.55488509074099	40.0	36.0	41.0	27.0	41.0
22	36.44928148774303	40.0	36.0	41.0	27.0	41.0
23	36.15880699867332	39.0	36.0	41.0	27.0	41.0
24	36.45894194925576	40.0	36.0	41.0	27.0	41.0
25	35.205896120419744	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.14230933499479903
1101	2	0.046929428238694726
1101	3	-0.10711530324314111
1101	4	-0.009675256224703332
1101	5	-0.2193931320543001
1101	6	-0.3011265034838857
1101	7	-0.2893206028890205
1101	8	-0.2618969258339874
1101	9	0.007044256552710237
1101	10	0.1329234012720022
1101	11	0.04075295370238052
1101	12	0.054343264004835135
1101	13	0.07787576569678123
1101	14	0.41961555947559503
1101	15	0.19701343910155344
1101	16	0.10026854635008675
1101	17	0.22296247007481895
1101	18	0.29304683877417403
1101	19	0.0544992347701978
1101	20	0.11902709681559998
1101	21	0.05329713681828707
1101	22	0.06130260011057942
1101	23	0.20949118123255062
1101	24	0.008144289288658513
1101	25	-0.010812803774392421
1102	1	-0.322899981619134
1102	2	-0.060639225817482156
1102	3	-0.3034161315828463
1102	4	-0.1698910056397196
1102	5	-0.5872741573586566
1102	6	-0.39610930659624444
1102	7	-0.4342190656519591
1102	8	-0.4852321946017497
1102	9	-0.23288057064934264
1102	10	0.08220565112740275
1102	11	-0.018962599265094582
1102	12	-0.18707023640914144
1102	13	-0.36596525288507564
1102	14	-0.08541113659168786
1102	15	-0.14639483282904564
1102	16	-0.2592872068083878
1102	17	-0.35319116032615483
1102	18	0.01367034469092232
1102	19	-0.13267451197043556
1102	20	-0.13211182109856168
1102	21	-0.2021802082003461
1102	22	-0.2316928509627445
1102	23	-0.21241853609097205
1102	24	-0.25481520542243175
1102	25	-0.3525190925123596
1103	1	-0.4836669985481734
1103	2	-0.3005607860118502
1103	3	-0.6341300947604545
1103	4	-0.43308601439252215
1103	5	-0.6028997114106858
1103	6	-0.6278266914309683
1103	7	-0.6770544138534902
1103	8	-0.5059030158189728
1103	9	-0.07411700585764436
1103	10	-0.03182792031275028
1103	11	-0.21991428865320728
1103	12	-0.32105693088435316
1103	13	-0.26814616652061574
1103	14	-0.37776168779587493
1103	15	-0.32689271277155996
1103	16	-0.43635454797834683
1103	17	-0.2789703757356605
1103	18	-0.03424769137087935
1103	19	-0.2765597759409246
1103	20	-0.230171620466038
1103	21	-0.2647409625811079
1103	22	-0.2923668931251697
1103	23	-2.488660963387737
1103	24	-1.2140731250820878
1103	25	-0.7912774655737067
1104	1	-0.23120735708573292
1104	2	-0.03393423017955044
1104	3	-0.2354145297750918
1104	4	-0.41865947290992267
1104	5	-0.32874496012177445
1104	6	-0.3471880955151647
1104	7	-0.27477540780953547
1104	8	-0.26813661530511723
1104	9	-2.4144992545926485
1104	10	-0.6528556323210566
1104	11	-0.46374914407996215
1104	12	-0.2694909683780793
1104	13	-0.08154998422472914
1104	14	0.06779605438097747
1104	15	-0.004491255935121785
1104	16	-0.07307905761143729
1104	17	0.29510620384198205
1104	18	0.4207843733804424
1104	19	0.006626985433776156
1104	20	-0.11244820072700179
1104	21	-0.010680372671679095
1104	22	-0.07781547506709074
1104	23	-0.09097061629577752
1104	24	0.06991441800972353
1104	25	-0.0014963668648420025
1105	1	-0.2845216736380429
1105	2	-0.3295745902991314
1105	3	-0.3576012882705406
1105	4	-0.14540666108182876
1105	5	-0.26474475453495216
1105	6	-0.27871295010532293
1105	7	-0.40748975348664374
1105	8	-0.4401045094964289
1105	9	-2.3011374817489525
1105	10	-0.7333327650701804
1105	11	-0.5151571568263407
1105	12	-0.33871410717488004
1105	13	-0.30824200659942846
1105	14	-0.27439550114031164
1105	15	-0.3884808395361432
1105	16	-0.2829295788596937
1105	17	-1.5609436166978554
1105	18	-0.3927079470951469
1105	19	-0.4094344850259617
1105	20	-0.3800502874332423
1105	21	-0.32945935827203954
1105	22	-0.2524583964558289
1105	23	-0.181237279535992
1105	24	-0.18621575271299662
1105	25	-0.7422500138893966
1106	1	-0.4727218526214827
1106	2	-0.5851315675137805
1106	3	-0.6473569705944051
1106	4	-0.5190648995345839
1106	5	-0.489719933641247
1106	6	-0.574691592215423
1106	7	-0.595544019285974
1106	8	-0.6388188099531007
1106	9	-3.131040729859791
1106	10	-0.9230763986815802
1106	11	-0.8001688679200569
1106	12	-0.5836381320345438
1106	13	-0.553952419253271
1106	14	-0.9051853670446945
1106	15	-0.8049244927858439
1106	16	-0.817972858672114
1106	17	-0.7199986336306665
1106	18	-0.3836520656399145
1106	19	-0.5446610981929467
1106	20	-0.6437630235593375
1106	21	-0.5496913669689505
1106	22	-0.5951321331565183
1106	23	-0.4376714466996887
1106	24	-0.6397347138737359
1106	25	-0.9192973440348595
1107	1	-0.3483004968713317
1107	2	-0.1707558430098466
1107	3	-0.20903400062314503
1107	4	-1.4677305720086622
1107	5	-0.506052095676047
1107	6	-0.30535539823043223
1107	7	-0.20259016751138859
1107	8	-0.09519344801927332
1107	9	0.09048827750503108
1107	10	0.31992425437118754
1107	11	0.04017333559853853
1107	12	-0.6428201513677436
1107	13	-0.29771742865905537
1107	14	0.08251155338388116
1107	15	-0.6486292835433431
1107	16	-1.015957034133315
1107	17	-0.26605349665842226
1107	18	-0.22497745088118393
1107	19	-0.11731594227938302
1107	20	-0.5253496958629995
1107	21	-0.2168639123412035
1107	22	-0.24219661833492978
1107	23	0.06917390339474139
1107	24	-0.21874863144685008
1107	25	-0.41797073427486
1108	1	0.3459935276614985
1108	2	0.3095113011966326
1108	3	0.39131606908459915
1108	4	0.7735566601298416
1108	5	0.6545822164653643
1108	6	0.5734255690832057
1108	7	0.5225971901937854
1108	8	0.44050944948682513
1108	9	0.8285452015065431
1108	10	0.8072981765428011
1108	11	0.6246578801847136
1108	12	0.4936654915181933
1108	13	0.4836247905135238
1108	14	0.6120331625851563
1108	15	0.5654519314014053
1108	16	0.7376341706096028
1108	17	0.6968231674935268
1108	18	0.7530897750173438
1108	19	0.5675448220089052
1108	20	0.5026865002654262
1108	21	0.446654203834548
1108	22	0.47648591159430254
1108	23	0.706725358678618
1108	24	0.47904553981280174
1108	25	0.7093162645149746
1109	1	0.3341367859915536
1109	2	0.23239599838884217
1109	3	0.3790403144932384
1109	4	0.7374382398879291
1109	5	0.5286312478139763
1109	6	0.453353454438151
1109	7	0.4029471066705099
1109	8	0.4679931319867521
1109	9	0.7806157128035238
1109	10	0.46363044178337987
1109	11	0.3574282374417521
1109	12	0.35757757177510996
1109	13	0.4165423692478214
1109	14	0.7645068254102583
1109	15	0.49127730623229837
1109	16	0.5711575421775308
1109	17	0.950638226949323
1109	18	0.6074129326632374
1109	19	0.4305807403774935
1109	20	0.5254959742329604
1109	21	0.44588874820160385
1109	22	0.47090064010526334
1109	23	0.6534785346383103
1109	24	0.46195240393580406
1109	25	0.4994655788890725
1110	1	0.38497403438977784
1110	2	0.3215890241672348
1110	3	0.44807450547864747
1110	4	0.606370935649899
1110	5	0.5979050512341217
1110	6	0.5726584737431679
1110	7	0.5639199261799135
1110	8	0.5938569749237672
1110	9	1.0942907118443799
1110	10	1.0393249516242307
1110	11	0.6329637032211224
1110	12	0.5171287605945949
1110	13	0.5251373754038013
1110	14	0.8100778319568747
1110	15	0.5753083756292199
1110	16	0.6853452490481615
1110	17	0.9174949791719342
1110	18	0.999507466221182
1110	19	0.6222375539855278
1110	20	0.6728572728137721
1110	21	0.6466815270431638
1110	22	0.6725259588037531
1110	23	0.7918360559226656
1110	24	0.6459297610838917
1110	25	0.7606274376119089
1111	1	0.18123921403391563
1111	2	0.12649110486125892
1111	3	0.2212794104786262
1111	4	0.5589034391014422
1111	5	0.4705331461148532
1111	6	0.3851559759942873
1111	7	0.32227725551886266
1111	8	0.250885778687028
1111	9	0.741588889959921
1111	10	0.5194286264482812
1111	11	0.30892134140011507
1111	12	0.08038273472068624
1111	13	0.20931732606791797
1111	14	0.23475181595819095
1111	15	0.2754373777527448
1111	16	0.12777708252419018
1111	17	0.5206626029105337
1111	18	0.5616387242460661
1111	19	0.27413793193205294
1111	20	-0.14563890368005872
1111	21	-0.25396705717992774
1111	22	-0.10504116080482362
1111	23	0.2708689577557237
1111	24	0.23309221265776614
1111	25	0.17289391382050212
1112	1	0.2702504202865583
1112	2	0.22367871702854458
1112	3	0.3357680484649386
1112	4	0.6848628935137029
1112	5	0.6331008685547914
1112	6	0.424309343579381
1112	7	0.46609385549614757
1112	8	0.5451760888640536
1112	9	-1.0906894990761202
1112	10	0.2345718298102355
1112	11	0.18396413588111216
1112	12	0.2770581576701687
1112	13	0.14111016423429135
1112	14	0.5463095380994005
1112	15	0.41735264667698146
1112	16	0.34580341951021154
1112	17	0.4344007067019291
1112	18	0.5622625771732714
1112	19	0.41374708822480244
1112	20	0.31605729996105936
1112	21	0.32774329561947013
1112	22	0.38987043450418213
1112	23	0.16604058782397857
1112	24	0.24366890054462687
1112	25	0.3576071514973975
1113	1	0.3335436560543883
1113	2	0.26974728905305767
1113	3	0.4344966754864146
1113	4	0.5702832113314855
1113	5	0.7267319320964916
1113	6	0.5020909034887495
1113	7	0.4516503907631986
1113	8	0.48085614603045457
1113	9	0.30813054332512735
1113	10	0.5837999477954696
1113	11	0.43692546396785303
1113	12	0.2332387790532522
1113	13	0.3628866143534637
1113	14	0.10643711623369256
1113	15	0.27563509662400065
1113	16	0.3205158841927016
1113	17	0.7591148274723665
1113	18	0.611110897866368
1113	19	0.4677940409825112
1113	20	0.5136299236333954
1113	21	0.4870360367870248
1113	22	0.5304527716888146
1113	23	0.2276768586997875
1113	24	0.35494720008567526
1113	25	0.3587809333384726
1114	1	0.41593460596508436
1114	2	0.3656701960298321
1114	3	0.4936637324681108
1114	4	0.6791009455006218
1114	5	0.694556616386933
1114	6	0.5243160309832788
1114	7	0.3973539306371734
1114	8	0.40461882795542436
1114	9	0.16317158536205767
1114	10	0.46110404621299494
1114	11	0.4807208672268075
1114	12	0.4275773075070859
1114	13	0.4296057701651179
1114	14	0.8182945566848261
1114	15	0.48083582047099327
1114	16	0.43572233045112085
1114	17	0.8249142634991387
1114	18	0.6789939031751118
1114	19	0.5523362083059027
1114	20	0.3886896877661101
1114	21	0.5176035139378286
1114	22	0.31320355069637174
1114	23	0.7588048881088838
1114	24	0.5239856875701321
1114	25	0.4964009003655363
1115	1	0.4434777531603018
1115	2	0.39327644343508794
1115	3	0.523667736316451
1115	4	0.7226933639785997
1115	5	0.7315041000045
1115	6	0.5119399546934957
1115	7	0.5219127832049466
1115	8	0.4633278096836406
1115	9	0.670615846814556
1115	10	0.4223198706118225
1115	11	0.3758207450841837
1115	12	0.37609698482570053
1115	13	0.2940278308231612
1115	14	0.3857159714977101
1115	15	0.33490606232604136
1115	16	0.4058076942294164
1115	17	0.7171135245137279
1115	18	0.827900606568285
1115	19	0.608495962163893
1115	20	0.6305074905738479
1115	21	0.3890244753500127
1115	22	-0.11306332989827439
1115	23	0.37584249799868275
1115	24	0.42122822385292835
1115	25	0.4927241320418432
1116	1	0.733795579454096
1116	2	0.6864154413057904
1116	3	0.8484607411249989
1116	4	1.1118773128325756
1116	5	1.105018962683296
1116	6	0.8880305307337224
1116	7	0.8678336477236925
1116	8	0.741766358671299
1116	9	1.0578477791586707
1116	10	0.8416297862990092
1116	11	0.6068417701627737
1116	12	0.635011416916555
1116	13	0.6372947020119994
1116	14	1.0722297143399686
1116	15	0.7701150244821093
1116	16	0.8847371527753296
1116	17	1.2091689667996732
1116	18	1.2988981187910298
1116	19	0.889832038343016
1116	20	0.7989512408152493
1116	21	0.9728142471065198
1116	22	0.9484853090405636
1116	23	1.2126820614507494
1116	24	0.8725130668579482
1116	25	0.8412117754216766
1201	1	-0.32842619516192073
1201	2	-0.07745626753647983
1201	3	-0.38126405144878106
1201	4	-0.19523572731689143
1201	5	-0.4977857820146738
1201	6	-0.45981722341568343
1201	7	-0.5557672151455577
1201	8	-0.47578013845368616
1201	9	-0.11690997879990306
1201	10	-0.03011851480532357
1201	11	-0.11251448638380879
1201	12	-0.14600004496080032
1201	13	-0.1407381932538314
1201	14	0.18703265164739946
1201	15	-0.044021011165391144
1201	16	-0.19295167229358157
1201	17	-0.06714743537774837
1201	18	0.046028385462349775
1201	19	-0.1993068873425301
1201	20	-0.14409653239351172
1201	21	-0.32084048823774225
1201	22	-0.24546162471705912
1201	23	-0.3453771785084996
1201	24	-0.4343543538988328
1201	25	-0.5151598312201884
1202	1	-0.5533240897891751
1202	2	-0.5248570518125213
1202	3	-0.7070469927775633
1202	4	-0.5213429366235616
1202	5	-0.9996161376083137
1202	6	-0.7931244631537595
1202	7	-0.7885530501272555
1202	8	-0.7535766911627491
1202	9	-0.12976170845156787
1202	10	-0.02375694967010844
1202	11	-0.2838461673452244
1202	12	-0.3695422738743659
1202	13	-0.3535952617485236
1202	14	-0.4446747370444015
1202	15	-0.5006775473774283
1202	16	-0.3917891392261197
1202	17	-0.10712042083206796
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2211	13	0.06707267158864738
2211	14	0.31414573349312747
2211	15	0.04501357553333207
2211	16	0.22360572600665307
2211	17	0.4233206963900571
2211	18	0.5786703801038087
2211	19	0.15820609809149744
2211	20	0.3428990014450122
2211	21	0.13459279144628766
2211	22	0.16935802724522375
2211	23	-1.802262356620595
2211	24	-0.4585463502638305
2211	25	-0.2198673946008256
2212	1	0.034765112330987336
2212	2	-1.3852320826538715
2212	3	-0.27618978178832165
2212	4	0.35181691782509716
2212	5	0.43646091847631396
2212	6	0.3314333727325476
2212	7	0.319714019775617
2212	8	0.3094138186307447
2212	9	0.7881503700471981
2212	10	0.5635392080100701
2212	11	0.31665743141887503
2212	12	0.2477666370769711
2212	13	0.1547183926523843
2212	14	0.5267112022095475
2212	15	0.2544185850343368
2212	16	0.3583340938868744
2212	17	0.6494647122140478
2212	18	0.7951185503041174
2212	19	0.4388398705878416
2212	20	0.4480019310683474
2212	21	0.3203480315407674
2212	22	0.33308816789143236
2212	23	0.6118791169844897
2212	24	0.34258769822440627
2212	25	0.2779553745108885
2213	1	0.25080368559848765
2213	2	0.19956248499267915
2213	3	0.2917270801781555
2213	4	0.4838971983747129
2213	5	0.40899300871196687
2213	6	0.31814630298551094
2213	7	0.3222250337630044
2213	8	0.26543863910470833
2213	9	0.4808157619073299
2213	10	0.5194644774316259
2213	11	0.27486465523037396
2213	12	0.20718702411856071
2213	13	0.13760920773123075
2213	14	-1.3055427396203783
2213	15	-0.315714209617191
2213	16	0.05023775888651727
2213	17	0.4794536371594731
2213	18	0.27341665132170334
2213	19	0.18995728506265408
2213	20	0.20350857909684095
2213	21	0.25559133938257617
2213	22	0.19299229930619788
2213	23	0.4652817811734451
2213	24	0.22929399174054055
2213	25	0.0808317316429168
2214	1	0.4287745942731789
2214	2	0.4275243674076137
2214	3	0.5288977585084993
2214	4	0.6482411910461039
2214	5	0.6622336271709841
2214	6	0.4542179270489797
2214	7	0.42599804330789226
2214	8	0.27745137766927996
2214	9	0.3723873715314241
2214	10	0.32067396327239805
2214	11	0.18479227504457896
2214	12	0.13333234043918196
2214	13	0.053105746302371415
2214	14	0.30071928248070634
2214	15	0.16971335883501837
2214	16	0.2475002703440694
2214	17	0.30102248154371836
2214	18	0.49917085829061136
2214	19	0.22916873545007377
2214	20	0.36908001782199307
2214	21	0.3254024037440928
2214	22	0.40616806056142707
2214	23	0.5046721743583689
2214	24	0.42682435696186616
2214	25	0.33667453705455586
2215	1	0.43979008536950914
2215	2	0.322556250070523
2215	3	0.42231209710754314
2215	4	0.6605846305285539
2215	5	0.698271237661686
2215	6	0.5482140073549715
2215	7	0.5661055242734392
2215	8	0.39528765295290924
2215	9	0.6139434848219594
2215	10	0.7508329155618654
2215	11	0.4264128337546538
2215	12	0.389775868791574
2215	13	0.18137982538026876
2215	14	0.4252555599223271
2215	15	0.2340822024337328
2215	16	0.2905213970631877
2215	17	0.6159644021937822
2215	18	0.8312759903170743
2215	19	0.42832349012261517
2215	20	-0.45076874633654995
2215	21	0.09825966711235168
2215	22	-0.5735358501736556
2215	23	0.34972560422141896
2215	24	0.2319121446366097
2215	25	0.4278614052295069
2216	1	0.28320123221052285
2216	2	-0.12795737040647026
2216	3	0.35383701164760595
2216	4	0.45448972817361977
2216	5	0.521656984474177
2216	6	0.41233321044572335
2216	7	0.47269780460087674
2216	8	0.42222063280603095
2216	9	0.694538451009933
2216	10	0.43048005040031256
2216	11	0.3400627421631697
2216	12	-0.004785783369996466
2216	13	0.10503841378318413
2216	14	0.2549579355185472
2216	15	0.21622788925291303
2216	16	0.36253356399844705
2216	17	0.3794464118333849
2216	18	0.7450638625587302
2216	19	0.44630964371238235
2216	20	-0.6642564408527178
2216	21	-0.9403751763732515
2216	22	-1.019812272261774
2216	23	-1.4679690613827745
2216	24	-1.152694980040529
2216	25	-0.8929168998521604
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9542.0
3	1188.0
4	1195.0
5	860.0
6	992.0
7	1413.0
8	753.0
9	1663.0
10	3418.0
11	6315.0
12	10307.0
13	15158.0
14	20375.0
15	24950.0
16	28329.0
17	29913.0
18	31172.0
19	31284.0
20	31184.0
21	31630.0
22	32213.0
23	33600.0
24	35326.0
25	37789.0
26	41622.0
27	46945.0
28	54916.0
29	65948.0
30	82738.0
31	104286.0
32	136390.0
33	184204.0
34	256384.0
35	364871.0
36	544391.0
37	898893.0
38	1617385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46401772682995	21.494508572511446	13.83163168620984	23.20984201444876
2	15.403456255382778	22.10584753498714	40.092891207088385	22.397805002541702
3	18.094344607724032	27.057975531588635	30.068629894301672	24.779049966385656
4	12.856406502354922	18.0308623398856	38.2307105169166	30.882020640842878
5	13.51443725078338	37.493613702136614	34.898667963539005	14.093281083541005
6	30.145939173481867	38.83381488388864	17.998436053708815	13.021809888920673
7	25.17876553768947	32.701460875409296	24.217694524699677	17.902079062201558
8	23.720598509245118	36.823373970407715	21.574382088452012	17.88164543189516
9	24.52427565705082	17.227410133670237	22.341861930788628	35.90645227849031
10	15.075731683467119	29.893911286367047	33.83131734161099	21.199039688554844
11	31.71346807666744	25.98211792282231	23.94740007349939	18.35701392701086
12	20.711930987989856	27.60288863081514	31.81210241872655	19.873077962468454
13	25.946737754037585	24.696790828130684	27.11834315585634	22.238128261975387
14	20.831604769690795	23.528122420814785	28.26280134510526	27.37747146438916
15	22.62337952252061	30.914276861078843	24.40146401578965	22.060879600610896
16	22.290042939553903	31.283351728665142	25.540553225295188	20.886052106485764
17	20.55305736481762	30.183488951388775	28.445660290761914	20.817793393031693
18	20.537837454688752	30.260096396146402	29.032922200486748	20.169143948678098
19	21.120350342666324	28.697110118551283	29.386502671521065	20.79603686726133
20	22.34180111748028	28.510981563261133	28.535394055714182	20.61182326354441
21	22.835249472921074	29.04437040562695	27.449870765202423	20.670509356249557
22	21.69030762379246	28.548848960089234	29.13169428246782	20.629149133650486
23	21.9523913063833	29.12962918651576	28.200093423816412	20.717886083284522
24	21.6434795387247	28.02329439416716	28.696095079227835	21.637130987880305
25	22.35684068664436	28.411965803071066	27.93680957014481	21.294383940139763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12509.0
1	12509.0
2	10647.5
3	8786.0
4	8786.0
5	8786.0
6	9423.0
7	10060.0
8	10060.0
9	10060.0
10	14327.5
11	18595.0
12	18595.0
13	18595.0
14	30514.0
15	42433.0
16	42433.0
17	42433.0
18	68097.0
19	93761.0
20	93761.0
21	93761.0
22	136566.5
23	179372.0
24	179372.0
25	179372.0
26	233990.0
27	288608.0
28	288608.0
29	288608.0
30	348259.5
31	407911.0
32	407911.0
33	407911.0
34	461561.5
35	515212.0
36	515212.0
37	515212.0
38	554763.5
39	594315.0
40	594315.0
41	594315.0
42	591144.5
43	587974.0
44	587974.0
45	587974.0
46	563014.0
47	538054.0
48	538054.0
49	538054.0
50	509011.5
51	479969.0
52	479969.0
53	479969.0
54	434343.0
55	388717.0
56	388717.0
57	388717.0
58	335561.5
59	282406.0
60	282406.0
61	282406.0
62	232562.5
63	182719.0
64	182719.0
65	182719.0
66	142044.5
67	101370.0
68	101370.0
69	101370.0
70	75427.5
71	49485.0
72	49485.0
73	49485.0
74	35692.0
75	21899.0
76	21899.0
77	21899.0
78	15669.5
79	9440.0
80	9440.0
81	9440.0
82	6672.5
83	3905.0
84	3905.0
85	3905.0
86	2678.5
87	1452.0
88	1452.0
89	1452.0
90	969.5
91	487.0
92	487.0
93	487.0
94	285.0
95	83.0
96	83.0
97	83.0
98	51.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1442045737956013
2	0.28436311998111025
3	0.34304089475721966
4	0.3713423391683276
5	0.37860444000695503
6	0.3383724013609592
7	0.3415884745894942
8	0.35847804625418767
9	0.34449331492494517
10	0.37727651299646314
11	0.29172896511743235
12	0.3410282553819429
13	0.30994646379261764
14	0.32071512189332513
15	0.3611961468537882
16	0.3087637787988983
17	0.36103015597747673
18	0.3283092044845755
19	0.34179596318488353
20	0.30345207075693087
21	0.3455930044805087
22	0.2971651663166334
23	0.2798398686016223
24	0.31737455550755655
25	0.3115856236961935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4819542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.35181506445407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.51104380430942	62.83549499841992
2	10.970027556048803	16.312829201986116
3	2.4088648375796757	5.373104187569707
4	0.8314665021476925	2.4728417439989494
5	0.3769061709305532	1.4011828958840002
6	0.21264681845770575	0.9486406152007125
7	0.1395014874097509	0.7260532155174246
8	0.09452324026708815	0.5622379583705197
9	0.06769088910228768	0.45296464212735865
>10	0.35096848779540823	4.645096868909981
>50	0.022445045295231544	1.1521025551202444
>100	0.012795892689721217	1.6330842267142573
>500	5.876158928760195E-4	0.3314013311305037
>1k	4.1972532130306285E-4	0.6742657765244259
>5k	1.1192675234492221E-4	0.47869978252588996
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6392	0.13262671017287536	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5905	0.1225220155774138	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5408	0.11220983238656287	No Hit
ATTCCATTCCATTCCATTCCATTCC	5304	0.11005195099451359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.074885953893544E-5	0.0
2	0.0	0.0	0.0	2.074885953893544E-5	0.0
3	0.0	0.0	0.0	2.074885953893544E-5	0.0
4	0.0	0.0	0.0	8.299543815574176E-5	0.0
5	0.0	0.0	0.0	8.299543815574176E-5	0.0
6	0.0	0.0	0.0	8.299543815574176E-5	0.0
7	0.0	0.0	0.0	1.037442976946772E-4	0.0
8	0.0	0.0	0.0	1.037442976946772E-4	0.0
9	2.074885953893544E-5	0.0	0.0	1.2449315723361265E-4	0.0
10	2.074885953893544E-5	0.0	0.0	1.452420167725481E-4	0.0
11	2.074885953893544E-5	0.0	0.0	1.8673973585041898E-4	0.0
12	2.074885953893544E-5	0.0	0.0	1.8673973585041898E-4	0.0
13	2.074885953893544E-5	0.0	0.0	2.2823745492828987E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGG	25	0.006037294	18.998137	7
CGGCGTA	40	0.0052864975	14.247859	12
AGCCGCG	170	1.0913936E-11	11.182247	2
TACATCG	90	9.4718955E-5	10.561232	5
GGTATCA	3555	0.0	10.321241	1
CGCGATC	135	6.4761844E-7	9.84914	17
GTGGTAT	1835	0.0	9.842418	1
GCGTCAG	185	6.4210326E-10	9.762613	1
TGGTATC	1800	0.0	9.557715	2
TAGACAG	705	0.0	9.437696	5
GCGCGAT	145	1.714825E-6	9.169889	16
CTGTCGC	380	0.0	8.749142	9
TATTCCG	210	6.330083E-9	8.599859	5
ATACCGT	145	1.6933727E-5	8.516228	6
GTCTAAT	480	0.0	8.515526	1
GTATTAG	730	0.0	8.463983	1
TCTGTCG	375	0.0	8.359267	8
AGCCTCG	320	0.0	8.316796	2
GTATCAA	6260	0.0	8.290916	1
GTCTTAG	495	0.0	8.25748	1
>>END_MODULE