FastQCFastQC Report
Wed 25 May 2016
SRR1295245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819542
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT162390.33694073005277264No Hit
GGTATCAACGCAGAGTACTTTTTTT131850.2735737130208638No Hit
TATCAACGCAGAGTACTTTTTTTTT91910.19070276802235564No Hit
GTGGTATCAACGCAGAGTACATGGG71900.14918430008494585No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57700.11972091953965751No Hit
ATTCCATTCCATTCCATTCCATTCC57280.11884946743902221No Hit
GAATGGAATGGAATGGAATGGAATG50290.10434601462130633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4100.012.97664453
GGTATCA47750.011.7605171
CAGCGTC4850.011.1658392
AGTGCGC600.005878838211.0832978
GTGGTAT18550.010.7569141
TGGTATC17900.010.7215252
TAAATCG803.770634E-410.6884635
GCAGCGT5000.010.6421741
GTACCGC856.5985E-410.058796
ACGACTG800.0045159.5008565
CCGTTCA1002.7576264E-49.49996959
GGACGGC800.00451847969.4999696
GCGTCAG5650.09.4165834
TCCGTAC1554.261492E-79.1942813
TTACGTA1201.7638877E-48.7089384
TATACCG1000.00290432278.5507715
GATCGAA1000.0029065068.5500611
CTTACAC6450.08.5433173
TGGACCG1451.6889255E-58.518015
AGCACCG1356.7968125E-58.4452055
Produced by FastQC (version 0.11.5)