FastQCFastQC Report
Wed 25 May 2016
SRR1295244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066879
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG90360.12786408257450002No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN82440.11665687214964342No Hit
GTATCAACGCAGAGTACTTTTTTTT75000.10612888659902059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG701.0868971E-412.2203643
GTGGTAT27700.010.0542361
GGTATCA50550.09.6462261
TGGTATC26150.09.5591792
CGCTATC1054.503169E-49.0462712
CGGTGCA1606.7630026E-78.9052379
GCGTCAG2356.548362E-118.8984841
CGTCAGA2451.546141E-108.5347962
GTATCAA88600.08.3680091
TAGACAG9350.08.234045
GTCTACG2201.4531906E-88.2090361
GTCCTAG8050.08.1473141
TACGACC1050.0044943078.1471994
AGCGCAA2105.8291334E-88.14204810
GTCCGGA1304.1648326E-48.0428611
TAAGACT9100.08.0427474
GTATAGA11900.07.9076871
GTGTAGA10250.07.882361
TACACTG14250.07.870585
CGTGCAA1451.5655716E-47.86128710
Produced by FastQC (version 0.11.5)