FastQCFastQC Report
Wed 25 May 2016
SRR1295244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066879
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT199060.28168021555201384No Hit
GGTATCAACGCAGAGTACTTTTTTT166030.23494105389380518No Hit
TATCAACGCAGAGTACTTTTTTTTT114900.16258945426969953No Hit
GTGGTATCAACGCAGAGTACATGGG93020.13162812041921193No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71070.1005677329412319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.011.6765391
AGCGTCA5000.011.2110783
GTGGTAT27400.010.9589631
TGGTATC25600.010.7998662
CCCGACG951.6459814E-49.99982812
GCAGCGT5050.09.9728081
CAGCGTC5750.09.7487632
GTCGTAG1106.825095E-59.5023921
TTAGACT6050.08.6371334
CTAGGAC7400.08.6021783
CTTACAC9350.08.4339663
TACGACC1150.00111048688.2616054
TAGACAG10150.08.2372455
GTATCAA109700.08.1251091
GTCTTGG10500.08.0544091
GTATCGT1304.1889015E-48.0385516
ACCGCGT1304.190176E-48.0383238
TATTCCG3550.08.028945
ATTAGAC5700.08.000773
TACATCG2158.644565E-87.95425275
Produced by FastQC (version 0.11.5)