FastQCFastQC Report
Wed 25 May 2016
SRR1295243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556519
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN76630.11687604352248503No Hit
GTGGTATCAACGCAGAGTACATGGG76450.11660150759877307No Hit
GTATCAACGCAGAGTACTTTTTTTT69120.10542179470539169No Hit
ATTCCATTCCATTCCATTCCATTCC68880.10505574680710907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG604.098236E-412.6655349
GTGGTAT21400.010.7043621
TGGTATC21050.010.3842072
GGTATCA43700.010.331651
AGCCGTT1356.864082E-69.1473297
GATCGCC1107.160228E-48.63512911
GTCTTAC5400.08.6250141
GTCTTAG6000.08.5546061
TATACCG1000.00289384188.5542785
GTACTAG4950.08.4489941
GTATTAT8900.08.4371271
TAGACAG8700.08.4122535
ACCGAGT1150.00111233858.2601948
TTAGACA7050.08.224164
TCTATAC8600.08.0676173
GTATCAA75300.08.0156041
GTCTACG1909.1030415E-78.0043111
GTCCTAG7750.07.9720351
TATACTG13500.07.9558315
CTAAGAC6400.07.87076233
Produced by FastQC (version 0.11.5)