##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295243_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6556519
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25146499232291	31.0	30.0	33.0	26.0	34.0
2	30.25623596911715	31.0	30.0	34.0	26.0	34.0
3	30.466756521257697	31.0	30.0	34.0	26.0	34.0
4	33.73982230509818	35.0	35.0	37.0	28.0	37.0
5	34.050516745242405	37.0	35.0	37.0	28.0	37.0
6	33.93644935673945	36.0	35.0	37.0	28.0	37.0
7	33.97914960667391	37.0	35.0	37.0	28.0	37.0
8	34.00414808528733	37.0	35.0	37.0	28.0	37.0
9	35.12552667047865	39.0	35.0	39.0	27.0	39.0
10	35.1266888725557	39.0	35.0	39.0	27.0	39.0
11	35.53808797015611	39.0	35.0	39.0	28.0	39.0
12	35.53217644911881	39.0	35.0	39.0	28.0	39.0
13	35.46722033444881	39.0	35.0	39.0	28.0	39.0
14	36.13614053432927	39.0	36.0	41.0	27.0	41.0
15	36.48746812142236	40.0	36.0	41.0	27.0	41.0
16	36.46274341613286	40.0	36.0	41.0	27.0	41.0
17	36.131456036351	39.0	36.0	41.0	27.0	41.0
18	36.03314685734915	39.0	36.0	41.0	27.0	41.0
19	36.458664879946205	40.0	36.0	41.0	27.0	41.0
20	36.48832802894341	40.0	36.0	41.0	27.0	41.0
21	36.524756353180706	40.0	36.0	41.0	27.0	41.0
22	36.427762506293355	40.0	36.0	41.0	27.0	41.0
23	36.152030673593714	39.0	36.0	41.0	27.0	41.0
24	36.440407783459484	40.0	36.0	41.0	27.0	41.0
25	35.16436709784567	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1918194000467608
1101	2	0.024588739891246547
1101	3	-0.18126026636267412
1101	4	-0.11543987626222219
1101	5	-0.2519781330895938
1101	6	-0.5029312645771924
1101	7	-0.3977057408130378
1101	8	-0.3624864899069138
1101	9	-0.1498852448136816
1101	10	-0.0666045020907049
1101	11	-0.12764637382038302
1101	12	-0.16551723419382114
1101	13	-0.05132159002285164
1101	14	0.2034272853452137
1101	15	0.06003402964167037
1101	16	-0.15203162919371493
1101	17	-0.08661405308780701
1101	18	0.11883281046940652
1101	19	-0.21519886936403054
1101	20	-0.04733624412506998
1101	21	-0.2044681130710586
1101	22	-0.13082248312477418
1101	23	-0.07397909706389783
1101	24	-0.24248118602683633
1101	25	-0.3589891588388028
1102	1	-0.3823574575820494
1102	2	-0.1777599095627913
1102	3	-0.3273400153268895
1102	4	-0.3210530198410808
1102	5	-0.5408791325396862
1102	6	-0.725197035823065
1102	7	-0.6083087321667833
1102	8	-0.5180708568002501
1102	9	-0.5156462352325875
1102	10	-0.09361661083065087
1102	11	-0.28593197286258487
1102	12	-0.3635608354053872
1102	13	-0.4541570182785861
1102	14	-0.20805307216745206
1102	15	-0.29287514748340726
1102	16	-0.4846606674731646
1102	17	-0.6275621282266002
1102	18	-0.22821627013446744
1102	19	-0.44187277529030666
1102	20	-0.3731500987392522
1102	21	-0.32251490744789635
1102	22	-0.3799414301267845
1102	23	-0.3454661184475185
1102	24	-0.35141109706449924
1102	25	-0.5013427488989493
1103	1	-0.47508887438246106
1103	2	-0.24061071180402038
1103	3	-0.4632489644173603
1103	4	-0.4229751413072762
1103	5	-0.565960367807449
1103	6	-0.7900455909863311
1103	7	-0.660246336061455
1103	8	-0.5854563359502265
1103	9	-0.23042600499049115
1103	10	-0.14339405007766715
1103	11	-0.2612593506879648
1103	12	-0.4719883630725974
1103	13	-0.3773828090060931
1103	14	-0.4111495810017871
1103	15	-0.49162966377016915
1103	16	-0.46609366953195064
1103	17	-0.341034341871044
1103	18	-0.031611862967054094
1103	19	-0.3796291514930914
1103	20	-0.30494695955950135
1103	21	-0.40149689810275646
1103	22	-0.33891315855101567
1103	23	-2.6023237525477256
1103	24	-1.2917442915743393
1103	25	-0.7565838435427921
1104	1	-0.33849706932413426
1104	2	-0.1373263461006502
1104	3	-0.27050664748497866
1104	4	-0.5986276560526633
1104	5	-0.38075795906291887
1104	6	-0.5655136580310725
1104	7	-0.4938320292583782
1104	8	-0.41709550020277675
1104	9	-2.79188184722387
1104	10	-0.9629461925239795
1104	11	-0.5895935580718472
1104	12	-0.5066057702602009
1104	13	-0.3293776843271061
1104	14	-0.1157927284095166
1104	15	-0.3649436807126989
1104	16	-0.3551065693439881
1104	17	-0.04296346172672827
1104	18	0.11217166850546079
1104	19	-0.28053928566456676
1104	20	-0.2897196927123602
1104	21	-0.261931280135407
1104	22	-0.28090654546880245
1104	23	-0.2329738702388795
1104	24	-0.2019809620701878
1104	25	-0.324969624671553
1105	1	-0.2859660053914581
1105	2	-0.16856049783804394
1105	3	-0.22550143990851268
1105	4	-0.045887284853471044
1105	5	-0.1808012489823696
1105	6	-0.4177818452707598
1105	7	-0.36670176344126304
1105	8	-0.44699109742686005
1105	9	-2.239428405051541
1105	10	-0.6817727943857648
1105	11	-0.47189088660468315
1105	12	-0.3584174905602211
1105	13	-0.2608275792132062
1105	14	-0.2472487555801095
1105	15	-0.2127158680760033
1105	16	-0.33726242912909754
1105	17	-1.5745560436436676
1105	18	-0.3806477104943795
1105	19	-0.3031899225278991
1105	20	-0.2840161816029152
1105	21	-0.2323309414137782
1105	22	-0.25675899674881464
1105	23	-0.11182589996568737
1105	24	-0.17137357417953325
1105	25	-0.7628271827374888
1106	1	-0.3803580084072955
1106	2	-0.48171053492928095
1106	3	-0.43681734157345176
1106	4	-0.41725495245773914
1106	5	-0.3119674347168413
1106	6	-0.5360530619038144
1106	7	-0.5313585056737438
1106	8	-0.5222590886996983
1106	9	-3.204372634714659
1106	10	-0.9293066922178141
1106	11	-0.7048962233745186
1106	12	-0.46517212049321444
1106	13	-0.5059481803420454
1106	14	-0.788605162333063
1106	15	-0.7496394876307306
1106	16	-0.7938222997263864
1106	17	-0.6319465840431846
1106	18	-0.29431995743060924
1106	19	-0.5585488581185842
1106	20	-0.4847088210348218
1106	21	-0.32099269197460245
1106	22	-0.3616002122735864
1106	23	-0.23641565145843657
1106	24	-0.44718254338287977
1106	25	-0.7438283185302481
1107	1	-0.37070839928772514
1107	2	-0.16775820780623008
1107	3	-0.23466200329159292
1107	4	-1.5325868834827432
1107	5	-0.4575176285052791
1107	6	-0.3009634395059635
1107	7	-0.18751228467844072
1107	8	-0.14953004133635517
1107	9	0.12950983927436255
1107	10	0.43056030071979023
1107	11	0.03775655395277511
1107	12	-0.520695631581745
1107	13	-0.20810346857005868
1107	14	0.10605747680159539
1107	15	-0.7161591561862295
1107	16	-0.9918254218845775
1107	17	-0.1375108489665351
1107	18	-0.1270981036046379
1107	19	-0.058840311605955264
1107	20	-0.43307632512221517
1107	21	-0.09988159487856052
1107	22	-0.0867328549440387
1107	23	0.12328043765501207
1107	24	-0.2437268964890862
1107	25	-0.4589264881214632
1108	1	0.32502241442435675
1108	2	0.351569394703489
1108	3	0.411544814051215
1108	4	0.8086517324290199
1108	5	0.5276066707711706
1108	6	0.5117122288322165
1108	7	0.4373394649856337
1108	8	0.47119817263288155
1108	9	0.7578340376065142
1108	10	0.8161861925244338
1108	11	0.5636116929387427
1108	12	0.4631722862544976
1108	13	0.3993914043656517
1108	14	0.45345810534513475
1108	15	0.5654419530126304
1108	16	0.5360664949206821
1108	17	0.6470209967691787
1108	18	0.7825315017469734
1108	19	0.5335415040841625
1108	20	0.5357239845442905
1108	21	0.5215170463472703
1108	22	0.512089433691294
1108	23	0.7079848134678954
1108	24	0.5517226807555886
1108	25	0.5640491199769571
1109	1	0.3633080810104268
1109	2	0.2425748867417603
1109	3	0.38087906272426153
1109	4	0.6738577414327338
1109	5	0.5268709448895095
1109	6	0.49977035578235274
1109	7	0.44591185166197533
1109	8	0.4545250295059873
1109	9	0.8313132208241356
1109	10	0.520101860377018
1109	11	0.4059991176678821
1109	12	0.4652731060304447
1109	13	0.4437624969013143
1109	14	0.8587536909898432
1109	15	0.5695167978654254
1109	16	0.6582020285234336
1109	17	0.9285940282131122
1109	18	0.6463849380128508
1109	19	0.5046694443971091
1109	20	0.47139488825516196
1109	21	0.4870317666217545
1109	22	0.4662890583444437
1109	23	0.6452785741900371
1109	24	0.4354348150514724
1109	25	0.4298267845366155
1110	1	0.30148427553365664
1110	2	0.22631773303718106
1110	3	0.3085228209553712
1110	4	0.4607999834745726
1110	5	0.401661106077178
1110	6	0.45849815880231404
1110	7	0.3027201348593209
1110	8	0.4370262121355992
1110	9	0.9351822283321738
1110	10	0.8842036078345004
1110	11	0.5082066333109765
1110	12	0.452196421746919
1110	13	0.3213186934199186
1110	14	0.64421671599343
1110	15	0.47695130080457204
1110	16	0.4790645626754966
1110	17	0.8801936623136086
1110	18	0.9313172588621725
1110	19	0.5258128371396822
1110	20	0.5275953306535897
1110	21	0.5505203854591656
1110	22	0.559625363840702
1110	23	0.6655628509982066
1110	24	0.5187507348900127
1110	25	0.49497835669707513
1111	1	0.24495173519924762
1111	2	0.19384498942963546
1111	3	0.31461438907166794
1111	4	0.63513227267552
1111	5	0.5660837815815256
1111	6	0.46806454317718504
1111	7	0.36176359133298064
1111	8	0.3408084024940763
1111	9	0.8436420600710974
1111	10	0.6184018173277721
1111	11	0.39354848780116924
1111	12	0.29164511479064714
1111	13	0.34852706493176555
1111	14	0.32790244048228345
1111	15	0.2955516483129088
1111	16	0.213550684142227
1111	17	0.509544279826784
1111	18	0.596908396490555
1111	19	0.4342450502701567
1111	20	-0.1350075483290354
1111	21	-0.16331550190245991
1111	22	-0.01488977087627319
1111	23	0.24658705601967057
1111	24	0.284536022788501
1111	25	0.21413334587271038
1112	1	0.3354380436942108
1112	2	0.24543227911877352
1112	3	0.34613368085619456
1112	4	0.7509161373994573
1112	5	0.6863141900357661
1112	6	0.6764563173526525
1112	7	0.5071024409161424
1112	8	0.5674890259981495
1112	9	-1.0522383741666488
1112	10	0.3354885908323979
1112	11	0.31047103680577237
1112	12	0.3452937836900176
1112	13	0.32763521876541546
1112	14	0.6753350952876715
1112	15	0.43509427131560585
1112	16	0.42899260558751706
1112	17	0.5136888970969196
1112	18	0.5826098687385937
1112	19	0.49101052207342377
1112	20	0.4360733201735343
1112	21	0.39474497485206683
1112	22	0.47511720452358475
1112	23	0.20675913282343572
1112	24	0.3795607859371799
1112	25	0.4469372521810371
1113	1	0.32025577750509626
1113	2	0.24324782404035972
1113	3	0.33257482397096894
1113	4	0.5394140334561897
1113	5	0.6003548601495723
1113	6	0.527926536387227
1113	7	0.39814644045646475
1113	8	0.40344919216305897
1113	9	0.2398824844007308
1113	10	0.5564931893280729
1113	11	0.36390693003506414
1113	12	0.17277129179036166
1113	13	0.21878807715957294
1113	14	0.005235127783684845
1113	15	0.2174663026288144
1113	16	0.23361722254701078
1113	17	0.6729738619423173
1113	18	0.48493681965906177
1113	19	0.39282620633157705
1113	20	0.4421233363826502
1113	21	0.41684769589554804
1113	22	0.4258500245388106
1113	23	0.14538228268644104
1113	24	0.18114212631605398
1113	25	0.2562738912715403
1114	1	0.4611690752588302
1114	2	0.44071516659890264
1114	3	0.4340041062864053
1114	4	0.7174605188155496
1114	5	0.6634910593080932
1114	6	0.6604566024123812
1114	7	0.37338541466886
1114	8	0.3653096568788996
1114	9	0.22549147153576854
1114	10	0.4093403035148313
1114	11	0.4708722437987589
1114	12	0.4661045505092716
1114	13	0.4508052407726453
1114	14	0.8565482268334748
1114	15	0.5509294937556675
1114	16	0.4771887452064121
1114	17	0.8811989397924194
1114	18	0.7461878280299388
1114	19	0.5379680325654874
1114	20	0.5134438911351396
1114	21	0.5919575297607906
1114	22	0.3314018111094228
1114	23	0.7696560188974786
1114	24	0.6149781025783412
1114	25	0.6479718457745349
1115	1	0.43531296825562293
1115	2	0.384159511394067
1115	3	0.47258702696767685
1115	4	0.7635720565483766
1115	5	0.6560881350762813
1115	6	0.6852933791031148
1115	7	0.5967255506432352
1115	8	0.6244130793628173
1115	9	0.7648268096785316
1115	10	0.5368645071206544
1115	11	0.5289296728233737
1115	12	0.5334937910129014
1115	13	0.38889414714705595
1115	14	0.5008339230912
1115	15	0.497669555274463
1115	16	0.5275168801214178
1115	17	0.7794635159728784
1115	18	0.9007560939567583
1115	19	0.659793741023293
1115	20	0.6317540206240722
1115	21	0.5234448899625974
1115	22	-0.030076217417331463
1115	23	0.4904713671836589
1115	24	0.5108316976010343
1115	25	0.6525128261017912
1116	1	0.7284552833425053
1116	2	0.6109941879341712
1116	3	0.7719078235009214
1116	4	1.0813120953156599
1116	5	0.9662656736749966
1116	6	0.9519669308780863
1116	7	0.8470040972920359
1116	8	0.8280711735353208
1116	9	1.09589446912387
1116	10	0.9035663168456765
1116	11	0.668047128140266
1116	12	0.6417175923488756
1116	13	0.606067179689191
1116	14	1.159936690177048
1116	15	0.8173508587246943
1116	16	0.9223624224107212
1116	17	1.255613765421927
1116	18	1.2473209465118842
1116	19	0.9433211524815874
1116	20	0.8981754237541821
1116	21	0.9224493328166332
1116	22	0.8975057758006599
1116	23	1.21172750303392
1116	24	0.9178303720268559
1116	25	0.9211729071852588
1201	1	-0.21006039543291521
1201	2	0.04993152297540249
1201	3	-0.22055782480888197
1201	4	-0.10908280287144834
1201	5	-0.3663063991568194
1201	6	-0.497484840002258
1201	7	-0.4693315502124449
1201	8	-0.510810548463283
1201	9	0.12682352602695346
1201	10	0.15935554341412228
1201	11	-0.003726517659359274
1201	12	0.030851244469545236
1201	13	-0.04370246683370027
1201	14	0.3228756875316279
1201	15	0.04746843624486985
1201	16	-0.022616373217431374
1201	17	0.050324904288913785
1201	18	0.19269290811760698
1201	19	-0.1199607159950844
1201	20	-0.005697204144418322
1201	21	-0.18229122322262725
1201	22	-0.1815407188302487
1201	23	-0.1702501484242518
1201	24	-0.30413822331836116
1201	25	-0.3177591856429345
1202	1	-0.4579469545968031
1202	2	-0.38427766781462935
1202	3	-0.5239655613473744
1202	4	-0.38099204353967764
1202	5	-0.7761474661019534
1202	6	-0.8907473989095962
1202	7	-0.6330784195925858
1202	8	-0.6661265386918842
1202	9	-0.13477802032178232
1202	10	0.010693210110680695
1202	11	-0.3137005775704864
1202	12	-0.3433393634217978
1202	13	-0.27659797262729313
1202	14	-0.3694582090330627
1202	15	-0.4126187291788881
1202	16	-0.34234672737132854
1202	17	-0.060038864086941146
1202	18	-0.020789018351585753
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2211	12	-0.01999369707568377
2211	13	0.03312264869582293
2211	14	0.31717371906316316
2211	15	0.10783745080006923
2211	16	0.29388637944438756
2211	17	0.520588332622907
2211	18	0.5097024141857318
2211	19	0.28507342119790735
2211	20	0.42480127799235845
2211	21	0.1435278692329831
2211	22	0.3045133249612917
2211	23	-1.8688655542986865
2211	24	-0.4997766015991374
2211	25	-0.20079678504400533
2212	1	0.1028380877908397
2212	2	-1.3964192599642047
2212	3	-0.28890742350043297
2212	4	0.34484456806176667
2212	5	0.3277343057596198
2212	6	0.41325943101724505
2212	7	0.37832360253027986
2212	8	0.3535943488356921
2212	9	0.7837602229136138
2212	10	0.6746507208527532
2212	11	0.43859681094420466
2212	12	0.3148035279380892
2212	13	0.23889627165327454
2212	14	0.614322311522038
2212	15	0.4214618073441656
2212	16	0.4407982688544294
2212	17	0.7279163675934655
2212	18	0.8196172570519664
2212	19	0.4587913465101039
2212	20	0.5297310928852141
2212	21	0.44197838817792956
2212	22	0.4347778222339116
2212	23	0.7011306920308229
2212	24	0.37612039768254846
2212	25	0.3206110290742359
2213	1	0.37390325579295336
2213	2	0.26693035295373946
2213	3	0.3274941990707809
2213	4	0.5434920409803325
2213	5	0.5101694925581697
2213	6	0.5553202547103524
2213	7	0.49047445215793317
2213	8	0.4785906324775766
2213	9	0.6804371337656292
2213	10	0.7218825096457664
2213	11	0.451162436441372
2213	12	0.47599501002844136
2213	13	0.3200675431528097
2213	14	-1.0528273301840017
2213	15	-0.027213712924925915
2213	16	0.2579268413772837
2213	17	0.6692716724737267
2213	18	0.5179238603848191
2213	19	0.4035473926417623
2213	20	0.46522950154319176
2213	21	0.540238290820291
2213	22	0.5702804733482694
2213	23	0.7719473312141147
2213	24	0.5351079657701803
2213	25	0.4075410731570628
2214	1	0.4069856946402375
2214	2	0.3495541496582675
2214	3	0.3819335578633627
2214	4	0.6783184389764187
2214	5	0.5029624334452976
2214	6	0.574766352062305
2214	7	0.3838652592923282
2214	8	0.3023930110052433
2214	9	0.5839352645961355
2214	10	0.40009566417614906
2214	11	0.3266671301583983
2214	12	0.2828246426527272
2214	13	0.14914949197140714
2214	14	0.44594256914749053
2214	15	0.2589142776261468
2214	16	0.35173910543066
2214	17	0.3698132176743414
2214	18	0.4613615565610871
2214	19	0.35744434008858406
2214	20	0.3979492915683309
2214	21	0.4387182243734884
2214	22	0.42060963593503686
2214	23	0.4750375625415302
2214	24	0.4095359047465479
2214	25	0.4025845783311013
2215	1	0.41695487850169144
2215	2	0.30120887171180755
2215	3	0.3312591283592319
2215	4	0.6740455611907947
2215	5	0.5471061715908121
2215	6	0.6709127814566784
2215	7	0.566637385436195
2215	8	0.47376789713648293
2215	9	0.7633532992620644
2215	10	0.7478436822003189
2215	11	0.45903535152979913
2215	12	0.3376763813310717
2215	13	0.3151862799542755
2215	14	0.5010369423102006
2215	15	0.3434927695043868
2215	16	0.5248768865836766
2215	17	0.6716499992865153
2215	18	0.82677626156309
2215	19	0.40713328136675386
2215	20	-0.4465028524843788
2215	21	0.08596084223366063
2215	22	-0.5950128329470701
2215	23	0.32361890734837573
2215	24	0.2621292603952554
2215	25	0.4249016173652507
2216	1	0.24479076853396364
2216	2	-0.132163322110884
2216	3	0.27441026468977725
2216	4	0.5909030767249988
2216	5	0.4861422559955457
2216	6	0.6191269020528694
2216	7	0.5268865439840695
2216	8	0.5662567349911996
2216	9	0.8400806521328974
2216	10	0.5282177717641332
2216	11	0.3805239170933703
2216	12	0.041273149242186946
2216	13	0.22287622918192795
2216	14	0.40468836615070813
2216	15	0.2832969200517752
2216	16	0.4158769743544255
2216	17	0.46970905067293245
2216	18	0.7874072867151582
2216	19	0.5099842573828539
2216	20	-0.631408499339777
2216	21	-0.8487714522416141
2216	22	-0.8591851133790271
2216	23	-1.379624405156811
2216	24	-1.0908666815152515
2216	25	-0.6855556188182561
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13380.0
3	1705.0
4	1544.0
5	1157.0
6	1344.0
7	1832.0
8	865.0
9	1743.0
10	3749.0
11	7573.0
12	13082.0
13	19366.0
14	26924.0
15	33494.0
16	38203.0
17	40913.0
18	42655.0
19	43345.0
20	43327.0
21	43923.0
22	45072.0
23	46948.0
24	48797.0
25	52898.0
26	57853.0
27	65229.0
28	75655.0
29	90800.0
30	112573.0
31	141555.0
32	185839.0
33	249100.0
34	342233.0
35	485105.0
36	718569.0
37	1170022.0
38	2288147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80686815314382	21.230483896565435	13.467423097620498	23.495224852670248
2	15.63753213288967	21.545456241910372	39.7253679967279	23.09164362847206
3	17.876170095927893	26.562313950117737	30.338302009866748	25.223213944087618
4	12.765362666648702	17.52514236059868	38.257370938535864	31.452124034216762
5	13.561539452005844	37.20411244412266	35.52809441884114	13.70625368503036
6	30.582923515373416	38.414101676073074	17.542478790830156	13.460496017723358
7	25.63085173803803	32.362696839623375	24.45645228703794	17.549999135300652
8	24.00800138132203	36.54793787479687	21.54531728158764	17.89874346229346
9	24.624629864223476	16.852723649860547	22.190836473566257	36.33181001234972
10	14.986815208378498	29.67489047375841	33.99289442001002	21.345399897853078
11	32.17976839873759	25.631163845372146	23.783866969169853	18.405200786720414
12	20.81240819185045	27.27303892895109	31.840772720441336	20.073780158757124
13	26.26466936907522	24.321967692886272	26.979276604377855	22.434086333660655
14	20.89693540973111	23.112671694237513	28.193783660211132	27.796609235820245
15	22.65088034583156	30.743892993053368	24.33667143246396	22.268555228651117
16	22.450798713832665	30.95781094515184	25.459179364529543	21.13221097648595
17	20.668021991966935	29.96922122677605	28.308878850235942	21.053877931021077
18	20.698099085771982	30.019313512587907	28.907635012103654	20.374952389536453
19	21.337230773657414	28.51288082192137	29.19044344530745	20.959444959113764
20	22.490809516764703	28.347506058780848	28.377048400330395	20.784636024124055
21	22.962171854895203	28.867570805234926	27.279428161511955	20.89082917835792
22	21.737585978003278	28.421844597144645	28.984544344310688	20.856025080541393
23	22.05491527217724	28.94125057339476	28.08457318398481	20.919260970443194
24	21.753711851330703	27.88465864004414	28.531197239411465	21.83043226921369
25	22.476764875674128	28.23016157291498	27.779619801254253	21.51345375015664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16597.0
1	16597.0
2	13953.0
3	11309.0
4	11309.0
5	11309.0
6	11844.0
7	12379.0
8	12379.0
9	12379.0
10	17909.5
11	23440.0
12	23440.0
13	23440.0
14	38767.5
15	54095.0
16	54095.0
17	54095.0
18	87899.0
19	121703.0
20	121703.0
21	121703.0
22	177938.0
23	234173.0
24	234173.0
25	234173.0
26	307361.5
27	380550.0
28	380550.0
29	380550.0
30	460583.0
31	540616.0
32	540616.0
33	540616.0
34	612879.5
35	685143.0
36	685143.0
37	685143.0
38	740941.5
39	796740.0
40	796740.0
41	796740.0
42	796840.0
43	796940.0
44	796940.0
45	796940.0
46	765018.5
47	733097.0
48	733097.0
49	733097.0
50	699009.0
51	664921.0
52	664921.0
53	664921.0
54	606154.0
55	547387.0
56	547387.0
57	547387.0
58	474403.0
59	401419.0
60	401419.0
61	401419.0
62	333276.0
63	265133.0
64	265133.0
65	265133.0
66	207249.5
67	149366.0
68	149366.0
69	149366.0
70	110671.0
71	71976.0
72	71976.0
73	71976.0
74	51499.5
75	31023.0
76	31023.0
77	31023.0
78	21615.0
79	12207.0
80	12207.0
81	12207.0
82	8372.5
83	4538.0
84	4538.0
85	4538.0
86	2934.5
87	1331.0
88	1331.0
89	1331.0
90	840.5
91	350.0
92	350.0
93	350.0
94	209.0
95	68.0
96	68.0
97	68.0
98	43.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14962207842301686
2	0.28711882021542223
3	0.34550345999149856
4	0.37390267609992434
5	0.3816964459341916
6	0.340378789415542
7	0.3424988168264288
8	0.3606639437787033
9	0.3472574395041027
10	0.37750214709970337
11	0.2975359333207148
12	0.34336818058484997
13	0.31451140460357085
14	0.32570636949271403
15	0.3638973668801997
16	0.3128336850697756
17	0.3625399392574017
18	0.331212339962715
19	0.3455797199703074
20	0.3071446906506334
21	0.34753197542781467
22	0.30226405200686524
23	0.28524282473672385
24	0.3218323625692231
25	0.3177295757093055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6556519.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.88787233305234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.91699911761359	58.06921775356154
2	12.350863672600305	17.51052894452449
3	3.0979994196966634	6.58831762053982
4	1.0820249545505718	3.068097873574308
5	0.4737997412218397	1.6793327783583507
6	0.26587423398877796	1.130835525338675
7	0.16557804556260408	0.8216232748498743
8	0.11099326203001517	0.6294460950890194
9	0.07904530506564321	0.5043018144618442
>10	0.41253376594909513	5.190302747350247
>50	0.027526718894714423	1.3405223983298322
>100	0.01559489240317548	1.9958400669812921
>500	5.618410388583159E-4	0.27661618563324464
>1k	4.7538023130881283E-4	0.5725714297370993
>5k	1.2964915275933076E-4	0.622445491670348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	7663	0.11687604352248503	No Hit
GTGGTATCAACGCAGAGTACATGGG	7645	0.11660150759877307	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6912	0.10542179470539169	No Hit
ATTCCATTCCATTCCATTCCATTCC	6888	0.10505574680710907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.5251995761775417E-5	0.0
4	0.0	1.5251995761775417E-5	0.0	3.0503991523550834E-5	0.0
5	0.0	1.5251995761775417E-5	0.0	3.0503991523550834E-5	0.0
6	0.0	1.5251995761775417E-5	0.0	3.0503991523550834E-5	0.0
7	0.0	1.5251995761775417E-5	0.0	3.0503991523550834E-5	0.0
8	0.0	1.5251995761775417E-5	0.0	3.0503991523550834E-5	0.0
9	3.0503991523550834E-5	1.5251995761775417E-5	0.0	9.15119745706525E-5	0.0
10	3.0503991523550834E-5	1.5251995761775417E-5	0.0	9.15119745706525E-5	0.0
11	3.0503991523550834E-5	3.0503991523550834E-5	0.0	9.15119745706525E-5	0.0
12	3.0503991523550834E-5	3.0503991523550834E-5	0.0	9.15119745706525E-5	0.0
13	3.0503991523550834E-5	3.0503991523550834E-5	0.0	1.2201596609420334E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACCG	60	4.098236E-4	12.665534	9
GTGGTAT	2140	0.0	10.704362	1
TGGTATC	2105	0.0	10.384207	2
GGTATCA	4370	0.0	10.33165	1
AGCCGTT	135	6.864082E-6	9.147329	7
GATCGCC	110	7.160228E-4	8.635129	11
GTCTTAC	540	0.0	8.625014	1
GTCTTAG	600	0.0	8.554606	1
TATACCG	100	0.0028938418	8.554278	5
GTACTAG	495	0.0	8.448994	1
GTATTAT	890	0.0	8.437127	1
TAGACAG	870	0.0	8.412253	5
ACCGAGT	115	0.0011123385	8.260194	8
TTAGACA	705	0.0	8.22416	4
TCTATAC	860	0.0	8.067617	3
GTATCAA	7530	0.0	8.015604	1
GTCTACG	190	9.1030415E-7	8.004311	1
GTCCTAG	775	0.0	7.972035	1
TATACTG	1350	0.0	7.955831	5
CTAAGAC	640	0.0	7.8707623	3
>>END_MODULE