FastQCFastQC Report
Wed 25 May 2016
SRR1295243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556519
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT183770.2802859261141469No Hit
GGTATCAACGCAGAGTACTTTTTTT147270.22461614158366658No Hit
TATCAACGCAGAGTACTTTTTTTTT107150.16342513458742358No Hit
GTGGTATCAACGCAGAGTACATGGG84080.12823878036500772No Hit
ATTCCATTCCATTCCATTCCATTCC78680.12000270265364898No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70900.1081366499509877No Hit
GAATGGAATGGAATGGAATGGAATG70340.1072825381883283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA6200.011.9522183
GCAGCGT5650.011.9409331
GGTATCA57550.011.7726061
CAGCGTC6750.011.1189972
GTGGTAT23350.010.6621021
TGGTATC22350.010.6268542
TACGTCG750.002648069510.1345
GCGTCAG8200.09.1529024
GCACCGT2700.09.1478436
GCGCGCC1252.756842E-59.12011311
GTATTAG8500.08.9433351
GTCCTAC4450.08.7549221
GTATCAA102850.08.435191
TACATCG1606.4524575E-68.3130475
CCGGTCG1606.4564247E-68.3126679
TATACCG1502.5868705E-58.2338755
ACTGATC8450.08.2072668
CGTCAGA9150.08.2027255
GTCTACG2451.3933459E-98.1448221
TTACGCC1050.00451378578.143274
Produced by FastQC (version 0.11.5)