FastQCFastQC Report
Wed 25 May 2016
SRR1295242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208167
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN78750.12684903611645756No Hit
GTGGTATCAACGCAGAGTACATGGG72280.1164272803872705No Hit
GTATCAACGCAGAGTACTTTTTTTT69530.11199763150701326No Hit
ATTCCATTCCATTCCATTCCATTCC69050.11122445642973199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG802.872037E-511.8740149
TAACGCT951.3547444E-511.005234
GCGTCAG1856.002665E-1110.2756521
GGTATCA45550.09.5571461
AATCCGA2151.0459189E-98.83604711
GTATTAG9100.08.6693761
TAGACTG8100.08.565735
GTCCTAA6150.08.5003861
CTAATAC9350.08.4370283
GTCTTAT8100.08.3315251
CTACGAC1150.00110637648.2646643
ACACCGT2303.7089194E-98.2601836
GTACTAG4650.08.1763251
GTATTAA12150.08.1359481
GTATAGG8600.08.0681791
GTGGTAT25700.08.0625891
CCTACAC7450.08.0372473
GTCTTAG7100.08.0323761
CTAGTAC3550.08.0318563
CCAGTAC7050.07.9540063
Produced by FastQC (version 0.11.5)