##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6208167 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.361932112973122 31.0 30.0 33.0 26.0 34.0 2 30.42886459078823 31.0 30.0 34.0 26.0 34.0 3 30.348202939772722 31.0 30.0 34.0 26.0 34.0 4 33.40624181018326 35.0 33.0 37.0 27.0 37.0 5 33.87166292401606 35.0 35.0 37.0 28.0 37.0 6 34.07556964881905 37.0 35.0 37.0 29.0 37.0 7 34.00054009500711 37.0 35.0 37.0 28.0 37.0 8 34.01009959944699 37.0 35.0 37.0 28.0 37.0 9 35.018359042210044 39.0 35.0 39.0 27.0 39.0 10 35.00687755339056 38.0 35.0 39.0 27.0 39.0 11 35.531551744661506 39.0 35.0 39.0 28.0 39.0 12 35.506527611129016 39.0 35.0 39.0 28.0 39.0 13 35.47941542165344 39.0 35.0 39.0 28.0 39.0 14 36.16433433572261 39.0 36.0 41.0 27.0 41.0 15 36.53963706195404 40.0 36.0 41.0 27.0 41.0 16 36.5468900884915 40.0 36.0 41.0 27.0 41.0 17 36.21892371774148 40.0 36.0 41.0 27.0 41.0 18 36.12699481183415 39.0 36.0 41.0 27.0 41.0 19 36.53968764049034 40.0 36.0 41.0 27.0 41.0 20 36.546581140616865 40.0 36.0 41.0 27.0 41.0 21 36.604674616517244 40.0 36.0 41.0 27.0 41.0 22 36.521010630029764 40.0 36.0 41.0 27.0 41.0 23 36.2606403790362 40.0 36.0 41.0 27.0 41.0 24 36.52276203265795 40.0 36.0 41.0 27.0 41.0 25 35.310082347978074 39.0 34.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3056551139673722 1101 2 0.04510323018081053 1101 3 -0.3193710527578979 1101 4 -0.49748749079031995 1101 5 -0.4532168265829526 1101 6 -0.5647544776739011 1101 7 -0.47423763287736875 1101 8 -0.4455710578534138 1101 9 -0.29403039069357817 1101 10 -0.2064067372568843 1101 11 -0.13602989986204506 1101 12 -0.14898835919602504 1101 13 -0.11015625619562286 1101 14 0.18546861568699313 1101 15 0.002317228667848781 1101 16 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9 -1.0930581320280481 2208 10 0.07968520173028537 2208 11 0.13086077079055514 2208 12 0.18790417704629903 2208 13 0.04277359839957029 2208 14 -1.4075715305637644 2208 15 -0.30971391894344436 2208 16 -0.055544706443065195 2208 17 0.06382157701695945 2208 18 0.6743506659131384 2208 19 0.33679736222453727 2208 20 0.4012056271582267 2208 21 0.37805724361084003 2208 22 0.2961753159239038 2208 23 0.4237135913955967 2208 24 0.30257247303701007 2208 25 0.38124117345252984 2209 1 0.2573347283360832 2209 2 0.158856872235976 2209 3 0.23019949694112185 2209 4 0.4944170208458729 2209 5 0.2755609570805646 2209 6 0.40810328692387543 2209 7 0.31832542897804217 2209 8 0.2976054371502883 2209 9 0.7631753003065995 2209 10 -0.5429949042377515 2209 11 0.04910571418336929 2209 12 0.2448425890788144 2209 13 0.17672613647877 2209 14 0.20226832090929747 2209 15 0.2571664807292109 2209 16 -0.04043205236584413 2209 17 -0.02176445180962361 2209 18 0.5464892504940693 2209 19 0.3321010742697794 2209 20 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19 0.5569318676391575 2212 20 0.6061396186431622 2212 21 0.5109481353190546 2212 22 0.4970470896644983 2212 23 0.7682011103551503 2212 24 0.5026986542302154 2212 25 0.5259932011369486 2213 1 0.2254637332416003 2213 2 0.0685526048125169 2213 3 0.20524797369295555 2213 4 0.598197344156354 2213 5 0.496554252875292 2213 6 0.3940321557282118 2213 7 0.22666439446046382 2213 8 0.3452042786059053 2213 9 0.5466635940057927 2213 10 0.6515833034141636 2213 11 0.24031072084427763 2213 12 0.29299625860073064 2213 13 0.18221319639015832 2213 14 -1.2951268345442628 2213 15 -0.3695130061550387 2213 16 -0.021893967037115658 2213 17 0.3513394886959773 2213 18 0.1256912810677946 2213 19 0.1276179156039774 2213 20 0.15008463784910475 2213 21 0.19763863114097546 2213 22 0.15265526020195352 2213 23 0.4123651192326605 2213 24 0.18567424159162726 2213 25 0.034673089386970446 2214 1 0.4865062131263116 2214 2 0.4662683149209954 2214 3 0.5760090706428329 2214 4 1.0002916648631697 2214 5 0.7532016762498657 2214 6 0.6538202284157393 2214 7 0.5104400015249126 2214 8 0.4782181989360481 2214 9 0.6918704849940696 2214 10 0.5130430085200572 2214 11 0.38832577017682013 2214 12 0.27541266776442797 2214 13 0.22722537345364913 2214 14 0.5198699466883454 2214 15 0.3079202539246779 2214 16 0.47072844418497084 2214 17 0.4353363877909544 2214 18 0.6723160932039747 2214 19 0.3978403333288085 2214 20 0.56668014129108 2214 21 0.49494041466230243 2214 22 0.5773230315172029 2214 23 0.5954172763077494 2214 24 0.6044986197636675 2214 25 0.6032536191097861 2215 1 0.3976130170285437 2215 2 0.20039790650635325 2215 3 0.440665524292541 2215 4 0.8092515491307708 2215 5 0.6694904901407099 2215 6 0.6167243652350578 2215 7 0.5448214723561904 2215 8 0.4385787973947757 2215 9 0.7257252269227976 2215 10 0.7548239477398369 2215 11 0.4168050106218999 2215 12 0.36024244999310184 2215 13 0.3242106272637457 2215 14 0.48898745014130185 2215 15 0.3705293078914522 2215 16 0.4393760899213106 2215 17 0.6067709065848916 2215 18 0.7627839645903833 2215 19 0.41984410032821273 2215 20 -0.3769276853677681 2215 21 0.13094719925607734 2215 22 -0.5615718660924713 2215 23 0.33164877144105276 2215 24 0.21763808307721888 2215 25 0.3549734962458331 2216 1 0.24024945203750647 2216 2 -0.2197092960363669 2216 3 0.28345858250669664 2216 4 0.8273636742880797 2216 5 0.5647262306957757 2216 6 0.5619749788224553 2216 7 0.4786136414802229 2216 8 0.4342439780549867 2216 9 0.8607878283238861 2216 10 0.4666643515740816 2216 11 0.4372746572916242 2216 12 0.05104170266063335 2216 13 0.2190547349917793 2216 14 0.20617249460403997 2216 15 0.14712783342631042 2216 16 0.3331025880239551 2216 17 0.35576284342627673 2216 18 0.6851511124260838 2216 19 0.4025568561380055 2216 20 -0.6671779787510488 2216 21 -0.9349516496101273 2216 22 -0.9858045021638802 2216 23 -1.5482836437942566 2216 24 -1.199204589867044 2216 25 -0.8966731422019691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13692.0 3 1858.0 4 1506.0 5 1056.0 6 1408.0 7 1751.0 8 1303.0 9 2561.0 10 4698.0 11 7984.0 12 12817.0 13 18761.0 14 25331.0 15 31247.0 16 35170.0 17 37969.0 18 38859.0 19 39501.0 20 39735.0 21 40252.0 22 40794.0 23 42640.0 24 44645.0 25 48510.0 26 53179.0 27 60734.0 28 69436.0 29 84933.0 30 104823.0 31 133328.0 32 175137.0 33 234223.0 34 325075.0 35 462489.0 36 685746.0 37 1121382.0 38 2163634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73134264787925 21.387166293394323 13.85575543363749 23.025735625088934 2 15.265547529248789 21.467998205269765 40.82452246490719 22.441931800574256 3 17.592153680003623 27.28841838417414 30.18972340962149 24.929704526200748 4 13.029427689274343 17.944376414637254 38.739212531802046 30.286983364286357 5 13.434882797186148 38.017925554832445 35.128206952856225 13.418984695125186 6 30.53170973571731 39.02113055510245 17.820251816815176 12.626907892365068 7 25.462658036095654 33.15551291788768 24.18821641871397 17.193612627302702 8 23.632313330726813 37.247558167248606 21.511040353503454 17.609088148521124 9 24.619629966693218 17.2289877154064 22.22809284121666 35.92328947668372 10 14.868337593724013 30.162061962242255 33.95092016999298 21.01868027404075 11 32.00343673819482 25.92647952807386 24.03221756226866 18.037866171462664 12 20.861167780098427 27.498754755250598 31.948510160243316 19.691567304407656 13 26.171929357843982 24.583756040804246 27.19956711307054 22.04474748828123 14 20.743028701145626 23.421634189218985 28.411037181338145 27.42429992829724 15 22.586564450262074 31.031577721847302 24.358270546297742 22.02358728159288 16 22.159978898936963 31.377875496616813 25.649024156644362 20.813121447801862 17 20.42418932803218 30.336056675000656 28.53598636809192 20.703767628875248 18 20.424941382239524 30.354276772818494 29.191248677513816 20.029533167428166 19 21.152661603781546 28.88529871765895 29.296136745106143 20.66590293345336 20 22.33405309260265 28.682872859246793 28.5091254173389 20.473948630811655 21 22.84674428861707 29.157203204039583 27.467150575115536 20.52890193222781 22 21.47334410025976 28.75410910578812 29.226274489397852 20.546272304554268 23 21.83391522832855 29.1715602234257 28.303863740760004 20.690660807485738 24 21.553117946222994 28.007005186003532 28.776379560179706 21.663497307593772 25 22.230220873765223 28.524940546170342 28.000450611056394 21.244387969008045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16401.0 1 16401.0 2 13950.5 3 11500.0 4 11500.0 5 11500.0 6 12009.5 7 12519.0 8 12519.0 9 12519.0 10 18104.0 11 23689.0 12 23689.0 13 23689.0 14 39320.0 15 54951.0 16 54951.0 17 54951.0 18 88743.0 19 122535.0 20 122535.0 21 122535.0 22 178312.0 23 234089.0 24 234089.0 25 234089.0 26 306236.5 27 378384.0 28 378384.0 29 378384.0 30 456707.5 31 535031.0 32 535031.0 33 535031.0 34 602261.5 35 669492.0 36 669492.0 37 669492.0 38 719729.0 39 769966.0 40 769966.0 41 769966.0 42 768132.0 43 766298.0 44 766298.0 45 766298.0 46 728565.0 47 690832.0 48 690832.0 49 690832.0 50 652591.0 51 614350.0 52 614350.0 53 614350.0 54 554008.5 55 493667.0 56 493667.0 57 493667.0 58 424251.0 59 354835.0 60 354835.0 61 354835.0 62 293206.0 63 231577.0 64 231577.0 65 231577.0 66 179577.5 67 127578.0 68 127578.0 69 127578.0 70 93549.5 71 59521.0 72 59521.0 73 59521.0 74 42440.0 75 25359.0 76 25359.0 77 25359.0 78 17683.0 79 10007.0 80 10007.0 81 10007.0 82 6879.0 83 3751.0 84 3751.0 85 3751.0 86 2547.0 87 1343.0 88 1343.0 89 1343.0 90 867.0 91 391.0 92 391.0 93 391.0 94 236.5 95 82.0 96 82.0 97 82.0 98 50.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15838813614388916 2 0.3050175679874591 3 0.3680152289717722 4 0.3963005505489785 5 0.40332355750094995 6 0.36210366119339255 7 0.36585678188102866 8 0.3813202834266539 9 0.3695132556840046 10 0.40032750407648504 11 0.3179843583460303 12 0.3646164801945566 13 0.3361700804762501 14 0.34655962057721706 15 0.38500897285784996 16 0.3350586413026583 17 0.3842196899664587 18 0.35279334463779727 19 0.3666299569583099 20 0.329404798550039 21 0.37007702917785557 22 0.3234610151434393 23 0.30453433356415827 24 0.343450812453982 25 0.33847349789398384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6208167.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.21184406271911 #Duplication Level Percentage of deduplicated Percentage of total 1 79.32779212046167 54.90422778081191 2 13.587989634696507 18.808996394449164 3 3.873451033764867 8.042660668005364 4 1.4381357923855218 3.981441208144069 5 0.6463870186636167 2.236881876995607 6 0.33988942579716436 1.4114624362104284 7 0.19170640501305847 0.9287847666711792 8 0.1297912605723056 0.7186473989947327 9 0.08483073259594588 0.5284162292541146 >10 0.3433892234990505 4.1034556464716685 >50 0.022600907578692923 1.08216213467183 >100 0.012634779429810363 1.6179187589791109 >500 7.475717231980869E-4 0.3558599908808122 >1k 5.139308580957524E-4 0.6273434274977985 >5k 1.4016296053521348E-4 0.6517412819622481 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 7875 0.12684903611645756 No Hit GTGGTATCAACGCAGAGTACATGGG 7228 0.1164272803872705 No Hit GTATCAACGCAGAGTACTTTTTTTT 6953 0.11199763150701326 No Hit ATTCCATTCCATTCCATTCCATTCC 6905 0.11122445642973199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.832344233007907E-5 0.0 0.0 0.0 3 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 4 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 5 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 6 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 7 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 8 0.0 4.832344233007907E-5 0.0 1.6107814110026358E-5 0.0 9 0.0 4.832344233007907E-5 0.0 6.443125644010543E-5 0.0 10 0.0 4.832344233007907E-5 0.0 6.443125644010543E-5 0.0 11 0.0 4.832344233007907E-5 0.0 8.053907055013179E-5 0.0 12 0.0 4.832344233007907E-5 0.0 8.053907055013179E-5 0.0 13 0.0 4.832344233007907E-5 0.0 8.053907055013179E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGCG 80 2.872037E-5 11.874014 9 TAACGCT 95 1.3547444E-5 11.00523 4 GCGTCAG 185 6.002665E-11 10.275652 1 GGTATCA 4555 0.0 9.557146 1 AATCCGA 215 1.0459189E-9 8.836047 11 GTATTAG 910 0.0 8.669376 1 TAGACTG 810 0.0 8.56573 5 GTCCTAA 615 0.0 8.500386 1 CTAATAC 935 0.0 8.437028 3 GTCTTAT 810 0.0 8.331525 1 CTACGAC 115 0.0011063764 8.264664 3 ACACCGT 230 3.7089194E-9 8.260183 6 GTACTAG 465 0.0 8.176325 1 GTATTAA 1215 0.0 8.135948 1 GTATAGG 860 0.0 8.068179 1 GTGGTAT 2570 0.0 8.062589 1 CCTACAC 745 0.0 8.037247 3 GTCTTAG 710 0.0 8.032376 1 CTAGTAC 355 0.0 8.031856 3 CCAGTAC 705 0.0 7.954006 3 >>END_MODULE