FastQCFastQC Report
Wed 25 May 2016
SRR1295242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208167
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT183700.2959005452011842No Hit
GGTATCAACGCAGAGTACTTTTTTT142890.2301645558181666No Hit
TATCAACGCAGAGTACTTTTTTTTT105880.17054953579695906No Hit
GTGGTATCAACGCAGAGTACATGGG77510.12485166716681428No Hit
ATTCCATTCCATTCCATTCCATTCC77190.12433621711529345No Hit
GAATGGAATGGAATGGAATGGAATG67580.10885660775555814No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63360.102059110201127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3950.012.0265763
GGTATCA57600.011.0696041
CAGCGTC4350.010.9205082
GTAGCGA700.001491690210.8578436
GCAGCGT4500.010.1358641
GTCTTAG6750.09.9950891
TAGACGT1701.6656486E-89.5009184
ACTGTCG1304.2456413E-69.499778
TACACCG2002.6557245E-99.0260925
TAATACG950.0018234559.000874
ACACCGC1502.70308E-68.8672386
AGTGCGC1000.00290757118.5497938
TATTCCG2803.6379788E-128.4831695
ACCGTGT3150.08.4442418
CTGATCG1252.7464557E-48.3597989
TTACACT12000.08.2341294
GTACTAA5800.08.1917011
GTATCAA104300.08.1722231
AGACCCG1401.0386635E-48.1438425
TCTACGC1050.00451152078.1437093
Produced by FastQC (version 0.11.5)