FastQCFastQC Report
Wed 25 May 2016
SRR1295241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412014
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA291627.077914828136908No Hit
GTACATAAGCAGTGGTATCAACGCA108772.639958836350221No Hit
GCTTATGTACTCTGCGTTGATACCA63411.539025372924221No Hit
CTTATGTACTCTGCGTTGATACCAC45041.0931667370526243No Hit
GAGTACATAAGCAGTGGTATCAACG31830.7725465639517104No Hit
TATGTACTCTGCGTTGATACCACTG25770.6254641832559088No Hit
GTATCAACGCAGAGTACTTTTTTTT24510.5948826981607421No Hit
GCGTTGATACCACTGCTTATGTACT22270.5405156135471125No Hit
CCCATGTACTCTGCGTTGATACCAC19710.4783818025601072No Hit
CATAAGCAGTGGTATCAACGCAGAG19460.47231404758090745No Hit
CTGCTTATGTACTCTGCGTTGATAC17680.42911163212900527No Hit
ACGCAGAGTACATAAGCAGTGGTAT16960.41163649778891015No Hit
ACTCTGCGTTGATACCACTGCTTAT16830.40848126519972616No Hit
GGTATCAACGCAGAGTACTTTTTTT16740.4062968734072143No Hit
TATCAACGCAGAGTACTTTTTTTTT15510.37644351890955163No Hit
GTATCAACGCAGAGTACATAAGCAG14410.34974539700107277No Hit
GTACTGGTTCACTATCGGTCAGTCA14270.34634745421272095No Hit
GTGGTATCAACGCAGAGTACATGGG13560.32911503007179366No Hit
TATCAACGCAGAGTACATAAGCAGT13050.3167368099142262No Hit
GGTATCAACGCAGAGTACATAAGCA12880.3126107365283704No Hit
CATGTACTCTGCGTTGATACCACTG12690.3079992427441786No Hit
ATACAGGGTGACAGCCCCGTACACA11140.2703791618731402No Hit
AAGCAGTGGTATCAACGCAGAGTAC10360.25144776633803706No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10110.24538001135883733No Hit
GTGGTATCAACGCAGAGTACATAAG10020.2431956195663254No Hit
GGATACCACGTGTCCCGCCCTACTC9300.22572048522623017No Hit
GTACATGGGAAGCAGTGGTATCAAC8920.21649749765784657No Hit
ACATAAGCAGTGGTATCAACGCAGA8680.21067245287781483No Hit
GATACCACTGCTTATGTACTCTGCG7520.18251806977432805No Hit
ATCATGTACTCTGCGTTGATACCAC7120.17280966180760848No Hit
CCACTGCTTATGTACTCTGCGTTGA7120.17280966180760848No Hit
GTACATGATAAGCAGTGGTATCAAC6830.16577106603173677No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6770.16431480483672886No Hit
GTACTCTGCGTTGATACCACTGCTT6740.16358667423922488No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6520.15824704985752913No Hit
ATACCACTGCTTATGTACTCTGCGT6190.15023761328498544No Hit
GCAGTGGTATCAACGCAGAGTACAT6140.1490240622891455No Hit
GTTGATACCACTGCTTATGTACTCT6090.14781051129330558No Hit
GGTATCAACGCAGAGTACATGGGGG5950.1444125685049537No Hit
CCCATATTCAGACAGGATACCACGT5830.14150004611493786No Hit
GTATTTAGCCTTGGAGGATGGTCCC5700.13834481352575398No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5330.12936453615653837No Hit
GTTAATGATAGTGTGTCGAAACACA5310.12887911575820238No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5150.12499575257151456No Hit
AAAAACAAAAAAAAAAAAAAAAAAA5100.12378220157567461No Hit
GGTTAATGAGGCGAACCGGGGGAAC5100.12378220157567461No Hit
GTTCACTATCGGTCAGTCAGGAGTA4980.12086967918565875No Hit
AAACAAAAAAAAAAAAAAAAAAAAA4810.11674360579980292No Hit
TACCACTGCTTATGTACTCTGCGTT4790.11625818540146693No Hit
TGATACCACTGCTTATGTACTCTGC4740.11504463440562698No Hit
CAGTGGTATCAACGCAGAGTACATA4560.11067585082060317No Hit
GCAGAGTACATAAGCAGTGGTATCA4550.1104331406214352No Hit
TATTCAGACAGGATACCACGTGTCC4510.10946229982476323No Hit
ATCATTAACTGAATCCATAGGTTAA4440.10776332843058731No Hit
GCCTTGGAGGATGGTCCCCCCATAT4430.10752061823141933No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4400.10679248763391536No Hit
GAACAAAAAAAAAAAAAAAAAAAAA4370.10606435703641139No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN4360.1058216468372434No Hit
GTATCAACGCAGAGTACATGGGGGT4230.10266641424805953No Hit
GAGTACATGGGAAGCAGTGGTATCA4170.10121015305305159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA402.7582762E-416.62233213
CCGGTTT350.0021688616.28310214
TCGATGG508.70093E-515.1975612
CGGTTTA456.755534E-414.77540615
CTATTTA456.7617855E-414.77360417
GCTAAAA400.0052461314.2598871
AAAAGTA707.1797367E-613.5808462
ACCGATA500.001498076413.29786619
TGTCTAT500.001498076413.29786614
TCCCGGT500.001498076413.29786612
TCTATTT500.001498076413.29786616
ACACCTT500.001499454313.2962446
GACTAAG500.001500833313.29462318
GAACATA801.9737417E-613.0715641
CGAATGG801.992772E-613.06040319
GATAAAA604.0567972E-412.6754561
CGATGGC604.0861458E-412.66463413
CCGAATG751.4784426E-512.66154618
CCTTCGA604.0987806E-412.6600029
GAACAAA1900.012.5086741
Produced by FastQC (version 0.11.5)