##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295241_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412014
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.391979398758295	31.0	30.0	33.0	22.0	34.0
2	29.38437043401438	31.0	30.0	34.0	22.0	34.0
3	29.802676608076425	31.0	30.0	34.0	25.0	34.0
4	32.61723145330013	35.0	33.0	37.0	19.0	37.0
5	33.40140383579199	35.0	33.0	37.0	27.0	37.0
6	33.30396539923401	36.0	35.0	37.0	26.0	37.0
7	33.48029193182756	37.0	35.0	37.0	27.0	37.0
8	33.53026596183625	37.0	35.0	37.0	27.0	37.0
9	34.24119326042319	38.0	34.0	39.0	22.0	39.0
10	34.39717339702049	39.0	34.0	39.0	23.0	39.0
11	34.788342143713564	39.0	35.0	39.0	25.0	39.0
12	34.90012475304237	39.0	35.0	39.0	27.0	39.0
13	34.6932628502915	39.0	35.0	39.0	25.0	39.0
14	35.317447950797785	39.0	34.0	41.0	23.0	41.0
15	35.6136806030863	39.0	34.0	41.0	25.0	41.0
16	35.5520710461295	39.0	34.0	41.0	25.0	41.0
17	35.29951409418126	39.0	34.0	41.0	24.0	41.0
18	35.081480240962684	38.0	34.0	41.0	23.0	41.0
19	35.394695325886985	39.0	34.0	41.0	25.0	41.0
20	35.391765813783024	39.0	34.0	41.0	25.0	41.0
21	35.443611139427304	39.0	34.0	41.0	25.0	41.0
22	35.37416204303737	39.0	34.0	41.0	25.0	41.0
23	35.07760901328596	39.0	33.0	41.0	24.0	41.0
24	35.385797569985485	39.0	34.0	41.0	25.0	41.0
25	34.32389676078968	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20305961725183863
1101	2	0.18089590046795934
1101	3	0.37074974929083027
1101	4	0.576837725227783
1101	5	0.24641151406171247
1101	6	0.4786794299781576
1101	7	0.1194093148187747
1101	8	0.16691727127219735
1101	9	0.496965017617363
1101	10	0.42564640625056427
1101	11	0.19161048731125874
1101	12	0.14418208777252772
1101	13	0.34093263686634145
1101	14	0.7296260119428339
1101	15	0.38968531424944786
1101	16	0.3568957523435117
1101	17	0.5871064234449079
1101	18	0.6794699609219563
1101	19	0.2926737297184445
1101	20	0.5412667323341651
1101	21	0.3348978576921411
1101	22	0.26488872512678086
1101	23	0.43053836359496245
1101	24	0.4594345411408014
1101	25	0.3538107751271866
1102	1	-0.06495597558962629
1102	2	-0.004382676185485934
1102	3	-0.05666312025542197
1102	4	-0.09088300298335383
1102	5	-0.2593517491674433
1102	6	-0.12118381462318695
1102	7	0.2460625331140136
1102	8	0.11787842092621759
1102	9	0.22027159050890077
1102	10	0.4390084536742478
1102	11	0.40857678914542106
1102	12	0.3709620660552275
1102	13	0.3618593932191061
1102	14	0.44599031742077955
1102	15	0.5570743765435822
1102	16	0.41572101892567304
1102	17	0.3361897988043978
1102	18	0.2493356135087268
1102	19	0.13045729271907192
1102	20	0.21187249968642163
1102	21	0.11139137657665543
1102	22	-0.06381718715893925
1102	23	0.27555496960765424
1102	24	0.1970595405728801
1102	25	0.10940706955565105
1103	1	-0.40056355764140505
1103	2	-0.42608845442789445
1103	3	-0.5616770133473921
1103	4	-0.446275631849808
1103	5	-0.2888459122347413
1103	6	-0.32111824656537635
1103	7	-0.6207448985438972
1103	8	-0.21765579826367087
1103	9	0.287490312711256
1103	10	0.4945762363734474
1103	11	0.007435538125214691
1103	12	0.26558548531481563
1103	13	-0.048637915280899335
1103	14	-0.11790415010240451
1103	15	0.0722146371651462
1103	16	-0.05681375965603763
1103	17	0.2870756553616616
1103	18	0.05029529144576372
1103	19	-0.08140089686890661
1103	20	0.04380276828619856
1103	21	-0.05827628965900544
1103	22	0.07894694206974151
1103	23	-2.4665470597925108
1103	24	-1.101612133109107
1103	25	-0.7350017633329173
1104	1	-0.464462354426491
1104	2	0.07087021775548763
1104	3	-0.030348245572625387
1104	4	-0.31461158086774077
1104	5	-0.29359932451419013
1104	6	-0.24098080422933066
1104	7	-0.21173980984841023
1104	8	-0.14125878489990384
1104	9	-2.2184116228953457
1104	10	-0.742247846947997
1104	11	-0.07068069338664884
1104	12	-0.5281654063958428
1104	13	-0.19705185925769086
1104	14	-0.22713513883117997
1104	15	-0.21958638057395063
1104	16	-0.13395743207721011
1104	17	-0.06148809600562544
1104	18	0.14048171842060242
1104	19	-0.11989901833178607
1104	20	-0.3172615775548593
1104	21	-0.08203859526608426
1104	22	-0.15036774856611146
1104	23	0.013045844568544851
1104	24	0.13425841428234264
1104	25	0.18306786143523368
1105	1	-0.3269439257395632
1105	2	-0.48283692068556405
1105	3	-0.1475665838995397
1105	4	0.10740534974528515
1105	5	-0.12320174045559895
1105	6	-0.45915505638132714
1105	7	-0.05019275130463541
1105	8	-0.04741039949066561
1105	9	-1.7876630615218758
1105	10	-0.1541967697615192
1105	11	-0.3529939097275445
1105	12	-0.4353347600839612
1105	13	-0.034834234299303546
1105	14	0.01706517505097338
1105	15	0.03233733655164883
1105	16	0.27447458390224
1105	17	-1.3172188231659447
1105	18	0.15612351230466004
1105	19	0.1440592258304818
1105	20	0.057606449267794346
1105	21	-0.1134910135853886
1105	22	0.27373221814335835
1105	23	0.0717964984896966
1105	24	0.3569768239461055
1105	25	-0.3009526835783163
1106	1	-0.309988544626286
1106	2	-0.5215352047315811
1106	3	-0.42284653653471693
1106	4	-0.3152822976317893
1106	5	-0.41229813399193915
1106	6	-0.4347096024854693
1106	7	-0.48211347647638547
1106	8	-0.6110288064402312
1106	9	-2.9189856016645805
1106	10	-1.2200251947791259
1106	11	-0.7930478076277723
1106	12	-0.34920785502797
1106	13	-0.7622287608042058
1106	14	-0.8429227795849599
1106	15	-1.0061772046526798
1106	16	-0.7962102665004593
1106	17	-0.6123787304778858
1106	18	-0.3148859504516892
1106	19	-0.37125012565936544
1106	20	-0.261215452878929
1106	21	0.019056478835224766
1106	22	-0.2130204333679231
1106	23	0.15009391690276885
1106	24	-0.08403151593731906
1106	25	-0.6347164515315242
1107	1	-0.26337983527599107
1107	2	-0.0139836880286488
1107	3	0.1530378376345638
1107	4	-1.2645680048849854
1107	5	-0.4089678855893766
1107	6	-0.3550420858219212
1107	7	-0.040736186161424826
1107	8	-0.19221745652047417
1107	9	-0.004909948660220209
1107	10	0.757263643350619
1107	11	-0.08169394965929655
1107	12	-0.38905022819300683
1107	13	-0.3380614459738922
1107	14	-0.008881389003185802
1107	15	-0.47721905797406805
1107	16	-0.4386429969595582
1107	17	0.17121911925973166
1107	18	0.10205228857793713
1107	19	0.41795474984398595
1107	20	-0.1332310284974767
1107	21	-0.03485268636281802
1107	22	0.08841258261136176
1107	23	0.33098080168060307
1107	24	-0.05623514105916172
1107	25	-0.3714594538255298
1108	1	0.16519900828260958
1108	2	0.012669483361364087
1108	3	0.04387316065612268
1108	4	0.29170556859507
1108	5	0.15227990845194483
1108	6	-0.058803461648075483
1108	7	-0.04260476292878934
1108	8	0.09023176317570858
1108	9	0.31251452969543436
1108	10	0.4674352755870288
1108	11	0.2506099362883134
1108	12	0.03939885309007707
1108	13	-0.17222525787050103
1108	14	0.3483714571160519
1108	15	0.18933514805382146
1108	16	0.07941126097324513
1108	17	-0.1355735033223695
1108	18	0.19452088610726292
1108	19	0.11755508203540899
1108	20	-0.07642110427864424
1108	21	0.018886197335575616
1108	22	-0.23557786688516558
1108	23	-0.10079884467393896
1108	24	0.014225774543454861
1108	25	-0.31101618589238456
1109	1	0.04739891938674745
1109	2	0.07056291291139516
1109	3	0.10056823913722468
1109	4	0.3592293830990343
1109	5	0.4093131398164971
1109	6	0.46106519907477406
1109	7	0.30210854791912567
1109	8	0.18744418070737368
1109	9	0.6346966616273519
1109	10	0.38882344546527037
1109	11	0.32153271018778895
1109	12	0.499866340736169
1109	13	0.39309359066663774
1109	14	0.6597466412263557
1109	15	0.2853339627756739
1109	16	0.366732270899945
1109	17	0.7247270867021243
1109	18	0.6387060509567917
1109	19	0.3822555814209849
1109	20	0.16235579325579863
1109	21	0.21854020069967817
1109	22	-0.019998867225055506
1109	23	0.35644398918667264
1109	24	0.17886613849356792
1109	25	0.12002415714105297
1110	1	0.0885682622781303
1110	2	0.16805237679673368
1110	3	0.2508377779362192
1110	4	0.11915190451399127
1110	5	-0.10329039713398203
1110	6	0.1922030110123103
1110	7	-0.05732442558691275
1110	8	0.2079326672658084
1110	9	0.5877761258222449
1110	10	0.3319085259731267
1110	11	0.1518758359533976
1110	12	0.33793079124406944
1110	13	0.14953797811263314
1110	14	0.31108858623955626
1110	15	0.015761996579684023
1110	16	0.32124234709606725
1110	17	0.6592926184608245
1110	18	0.6086366651691577
1110	19	0.17406505132063899
1110	20	0.39130235868142194
1110	21	0.42629071258016893
1110	22	0.46660600525810736
1110	23	0.5132275665569068
1110	24	0.22373694118409304
1110	25	0.7218850583160616
1111	1	0.43780359667666957
1111	2	0.4238861344565592
1111	3	0.376410618699655
1111	4	0.32123081168306555
1111	5	0.38245223275382045
1111	6	0.25590465974021015
1111	7	0.3483170682764438
1111	8	0.3151789931836717
1111	9	0.7169835424640425
1111	10	0.2868494421671812
1111	11	0.10913445270756483
1111	12	0.35799119192533624
1111	13	0.2610509800616043
1111	14	0.18638488318615742
1111	15	-0.1931953893350169
1111	16	0.021416174638076768
1111	17	0.3087930837567683
1111	18	0.3195079098797109
1111	19	0.1058245723291904
1111	20	0.13304116085163287
1111	21	0.07609405919930623
1111	22	-0.20004895585017834
1111	23	-0.009255884709922668
1111	24	0.26033290627811567
1111	25	-0.013471288145055382
1112	1	0.4488334286574691
1112	2	0.49127405571349314
1112	3	0.3757358163046547
1112	4	0.9686278768421559
1112	5	0.7203633786430217
1112	6	0.6594672646972555
1112	7	0.6891736057268787
1112	8	0.519597959746882
1112	9	-0.8151541963544631
1112	10	0.35973909109409163
1112	11	0.23649186498325747
1112	12	0.35460179357651356
1112	13	0.2577231272080951
1112	14	0.6612369541920771
1112	15	0.3747926005011806
1112	16	0.2898028913617452
1112	17	0.4764825779407218
1112	18	0.2605319389995202
1112	19	0.7395409531023773
1112	20	0.3627619201472001
1112	21	0.4394652576659439
1112	22	0.34005233045067484
1112	23	0.35810038457799465
1112	24	0.2915386704533631
1112	25	0.506506819243036
1113	1	-0.5537122404274015
1113	2	-0.4219022400648491
1113	3	-0.35995380872812177
1113	4	-0.2678833546935451
1113	5	-0.0796344362332988
1113	6	-0.1119338366050755
1113	7	-0.3872642056532456
1113	8	-0.42222093720268816
1113	9	-0.6724699904284037
1113	10	-0.1407088592598953
1113	11	-0.6152999364327059
1113	12	-0.4890067196653405
1113	13	-0.516420304310131
1113	14	-0.8225865752196881
1113	15	-0.6559520896482809
1113	16	-0.5273568848815913
1113	17	-0.2992559938624453
1113	18	-0.28894144619408024
1113	19	-0.5559227301420933
1113	20	-0.19951191955212266
1113	21	-0.3357663654439236
1113	22	-0.10100614345894599
1113	23	-0.5155907263388855
1113	24	-0.31417979821195985
1113	25	-0.54032475142386
1114	1	0.15496509015395432
1114	2	0.19541802137306163
1114	3	-0.02922625454855421
1114	4	0.38088685514477305
1114	5	0.33795242307182605
1114	6	-0.08365726281766683
1114	7	-0.22535330094048334
1114	8	-0.22701969881259032
1114	9	-0.3146784527607096
1114	10	-0.01794484137204222
1114	11	-0.29324971283449486
1114	12	-0.16966547439530189
1114	13	-0.004096602899743118
1114	14	0.17147087232073233
1114	15	-0.08113268820348907
1114	16	-0.24953610744780974
1114	17	0.317642870946635
1114	18	0.15066123698445466
1114	19	-8.659705961733266E-4
1114	20	0.021532112095620448
1114	21	0.029120115464230878
1114	22	-0.24873576162200095
1114	23	0.11557542433190804
1114	24	-0.03694381609981434
1114	25	-0.09025595197425673
1115	1	0.1858341805506285
1115	2	0.31606728509461135
1115	3	0.49114470296571255
1115	4	0.8088707204304768
1115	5	0.4933811555455776
1115	6	0.3378181123672874
1115	7	0.16395833155441153
1115	8	0.2975522732863283
1115	9	0.31108777002517485
1115	10	0.5733237417323096
1115	11	0.423661792837315
1115	12	0.24184019741474572
1115	13	0.2535832392759829
1115	14	0.5437670746740082
1115	15	0.35503302729006236
1115	16	0.4664418394274108
1115	17	0.7371366354784499
1115	18	0.5939607508293108
1115	19	0.3060723088373507
1115	20	0.3550415885981266
1115	21	0.2209664481634519
1115	22	0.18843396993580797
1115	23	0.7315921227043489
1115	24	0.6684743477837856
1115	25	0.5102953761452369
1116	1	0.23631283937195846
1116	2	0.4119554890733177
1116	3	0.0037602815591561978
1116	4	0.20631865169040253
1116	5	0.16066424137727608
1116	6	0.06134477716601339
1116	7	-0.010189422251514202
1116	8	-0.03273165311695436
1116	9	0.40329380346124566
1116	10	0.23914750188921374
1116	11	-0.1854093755571924
1116	12	0.12144699670493253
1116	13	0.007599303533005752
1116	14	0.29069431853316274
1116	15	0.13700276671286815
1116	16	0.0086782268375174
1116	17	0.5075041520563914
1116	18	0.272264664157035
1116	19	0.2180854777922434
1116	20	0.2873895852358359
1116	21	0.2187365985323666
1116	22	0.24808186383699393
1116	23	0.667309410460021
1116	24	0.40712663133660243
1116	25	0.23423065213290784
1201	1	0.15830119996359215
1201	2	0.20514436492729615
1201	3	-0.12008210336941971
1201	4	0.3016444861163663
1201	5	-0.19676220722300286
1201	6	-0.04963675159896752
1201	7	-0.05584074606169054
1201	8	0.40822940317085
1201	9	0.5625535738934673
1201	10	0.5218195052016128
1201	11	0.5020242039059184
1201	12	0.6346666962885834
1201	13	0.5382620137209386
1201	14	0.7235514942514243
1201	15	0.5469192666093576
1201	16	0.5007675696479978
1201	17	0.5732392059979148
1201	18	0.23474942481307437
1201	19	0.19780392372420152
1201	20	0.23106496995738723
1201	21	0.14735896183832864
1201	22	0.33752588656373916
1201	23	0.37672905023643466
1201	24	0.18219391447786393
1201	25	0.3183242274555269
1202	1	0.1620261826013838
1202	2	0.22800836247403922
1202	3	0.1397467097771532
1202	4	0.4947708559268733
1202	5	0.28592519187326104
1202	6	0.265913472531345
1202	7	0.450716305700233
1202	8	0.12436407978282915
1202	9	0.7948373580428978
1202	10	0.8724549168888629
1202	11	0.6399387547884601
1202	12	0.6396228172558125
1202	13	0.6023308131581331
1202	14	0.5700515438577298
1202	15	0.5462202686567466
1202	16	0.46479848790582423
1202	17	0.8181094629585814
1202	18	0.8603210247517481
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2211	7	0.1396644346553373
2211	8	0.17701198994731016
2211	9	-1.3995805365842742
2211	10	-0.099999207291809
2211	11	-0.34165655955885654
2211	12	0.06469495474511433
2211	13	-0.23581848086838164
2211	14	0.3780774907047473
2211	15	-0.04104457671762418
2211	16	-0.14891601524013964
2211	17	0.34916821933997966
2211	18	0.2974243818062732
2211	19	-0.042483613810020415
2211	20	0.23538112857621485
2211	21	0.20959832290533598
2211	22	0.2795540388852231
2211	23	-1.4647184549265972
2211	24	-0.6855153816256561
2211	25	-0.30471503808994527
2212	1	0.004928272878945705
2212	2	-1.5636514434065063
2212	3	-0.29748933538387945
2212	4	0.1831695261952646
2212	5	0.37566872456249456
2212	6	-0.11997793272082902
2212	7	-0.005404331396071882
2212	8	-0.2646246922625437
2212	9	0.34407035924001406
2212	10	0.3808547442195618
2212	11	-0.04654186219276113
2212	12	-0.16715890293981772
2212	13	-0.10562666035181678
2212	14	-0.16160345576400204
2212	15	0.004563800455933631
2212	16	0.05100630519678617
2212	17	0.16775589141875713
2212	18	0.23304417089316587
2212	19	-0.2535887365153613
2212	20	0.025078985623999017
2212	21	-0.20006099984233572
2212	22	-0.20464493426077723
2212	23	0.17150771479452231
2212	24	0.05730198630272554
2212	25	0.4108250203442907
2213	1	0.05889249309596423
2213	2	-0.1222520716274289
2213	3	-0.06692236707657173
2213	4	-0.07744325795000151
2213	5	0.07551586177136471
2213	6	-0.043159542746565194
2213	7	-0.029060439565313345
2213	8	-0.2432497011311625
2213	9	0.15195343532955974
2213	10	0.035124690018847105
2213	11	-0.2836984852795581
2213	12	-0.5512573027179428
2213	13	-0.16567529264907677
2213	14	-1.7469862469826651
2213	15	-0.8482514763633162
2213	16	-0.5655252359224505
2213	17	0.03259379451105815
2213	18	-0.22260949491136728
2213	19	-0.4323328536628708
2213	20	-0.038280598829999235
2213	21	-0.061323504599144485
2213	22	-0.01769008575833908
2213	23	0.24274908535672068
2213	24	-0.21446268669775037
2213	25	-0.6237142305635359
2214	1	0.07802795565103082
2214	2	0.2023624658175045
2214	3	0.05904128931109298
2214	4	0.2997987181355839
2214	5	-0.038352923628259816
2214	6	0.2873483762800788
2214	7	-0.1588328331042277
2214	8	0.07326225607462789
2214	9	-0.028807525442410054
2214	10	-0.024549895674461197
2214	11	-0.014949796376683366
2214	12	-0.12123711182220376
2214	13	-0.29001974408603814
2214	14	0.09724885895398216
2214	15	-0.19814396639898035
2214	16	-0.28934918180036107
2214	17	-0.34228820674759675
2214	18	-0.22253738456942784
2214	19	-0.34210970493200676
2214	20	-0.18818488873979788
2214	21	-0.017559082530752335
2214	22	0.06877676970631796
2214	23	0.06858294312890223
2214	24	-0.09581495227358516
2214	25	-0.24820290268436196
2215	1	0.038183037863287694
2215	2	-0.1344275192348121
2215	3	0.11822477212125904
2215	4	0.3216730508283021
2215	5	0.05753913274585898
2215	6	0.041745931266198966
2215	7	-5.742381864379809E-4
2215	8	-0.2333921321380572
2215	9	-0.18182108512637285
2215	10	0.15649724291191802
2215	11	-0.2835009388067533
2215	12	-0.2702935393259409
2215	13	-0.1642990522218497
2215	14	0.02596042205455973
2215	15	-0.2291737549960331
2215	16	-0.5334568585033281
2215	17	-0.13316745082190806
2215	18	-0.14528322057199006
2215	19	-0.5421884471912435
2215	20	-1.402477276098331
2215	21	-0.5478014485354521
2215	22	-1.3597813127194769
2215	23	-0.39858553861357393
2215	24	-0.36796288095178653
2215	25	-0.2029995074249129
2216	1	0.039620491438352445
2216	2	0.017163079314435947
2216	3	-0.0156647434087418
2216	4	0.3205955337943607
2216	5	0.20501911844010579
2216	6	-0.09719186619727793
2216	7	-0.09313753658070567
2216	8	-0.15170487801827193
2216	9	0.05589522068671471
2216	10	-0.21247897835047524
2216	11	0.19761958720496864
2216	12	-0.5319605269551246
2216	13	-0.24035570669194328
2216	14	-0.1424440274030161
2216	15	0.03847230587680173
2216	16	-0.14730455720205526
2216	17	0.19965234247823105
2216	18	0.4490682975807303
2216	19	0.1547954221494976
2216	20	-1.0788966182168664
2216	21	-0.9263744491682075
2216	22	-0.9962064321633903
2216	23	-1.9021298818784018
2216	24	-1.5157225625569595
2216	25	-1.4037134197746397
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	789.0
3	90.0
4	107.0
5	67.0
6	82.0
7	120.0
8	52.0
9	83.0
10	189.0
11	451.0
12	892.0
13	1509.0
14	2332.0
15	3390.0
16	4373.0
17	5063.0
18	4691.0
19	4498.0
20	4337.0
21	4099.0
22	3972.0
23	4118.0
24	4350.0
25	4296.0
26	4506.0
27	4682.0
28	4977.0
29	5589.0
30	6681.0
31	8620.0
32	11285.0
33	14537.0
34	19772.0
35	28743.0
36	47063.0
37	80656.0
38	120953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28414142420699	24.44567048169491	11.14200508940485	20.128183004693238
2	14.009657132843985	30.197766788029828	33.3925546620977	22.400021417028483
3	19.08570203978258	34.972919791930494	26.068109645619437	19.873268522667498
4	12.720363445915497	26.611451469495634	28.761921001668632	31.90626408292023
5	11.757726948272627	46.63478521434714	27.605309894050613	14.002177943329622
6	27.48215033653799	39.34645393179627	18.33620681258097	14.835188919084771
7	22.38313831017814	42.10332777283963	17.408160531859192	18.10537338512304
8	21.041325172381036	41.653981143426385	19.951872139668218	17.35282154452436
9	24.928954270171023	27.537251826977165	15.557249343116176	31.976544559735636
10	15.175093484695307	35.62966662200514	24.82003434877404	24.37520554452551
11	26.87785228365238	35.00553723333049	17.361599591096617	20.75501089192051
12	22.420493839185703	34.827351093361905	22.00262991282326	20.749525154629133
13	26.361788370479474	29.721616982946454	24.53811790196341	19.378476744610666
14	24.367601731517414	32.641245757636256	20.81935443031811	22.17179808052822
15	27.207141378344474	32.44358384177901	20.45839270273891	19.890882077137604
16	21.730524839685067	33.66832702785609	23.615107826095425	20.986040306363417
17	19.356543681374887	37.34375152229616	22.130045498387585	21.16965929794137
18	19.323585629238245	31.982127856438474	29.283531574526105	19.410754939797172
19	21.35024364584779	37.05657308036051	21.447409756061923	20.145773517729772
20	20.735839686851868	34.34965286906396	25.424541621632144	19.48996582245202
21	22.207908278194886	35.659092182070125	21.340954087956888	20.792045451778108
22	20.592065389099705	34.469236891875774	20.892430744365715	24.046266974658803
23	23.464187495436732	31.559784857261068	21.476064153423057	23.49996349387914
24	20.724059015435557	33.58207138335687	20.535618639528654	25.158250961678917
25	22.594764606931676	35.52598586594804	21.171378423052673	20.707871104067618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30467.0
1	30467.0
2	17778.5
3	5090.0
4	5090.0
5	5090.0
6	4626.5
7	4163.0
8	4163.0
9	4163.0
10	3860.5
11	3558.0
12	3558.0
13	3558.0
14	3627.5
15	3697.0
16	3697.0
17	3697.0
18	4498.5
19	5300.0
20	5300.0
21	5300.0
22	6926.5
23	8553.0
24	8553.0
25	8553.0
26	10449.0
27	12345.0
28	12345.0
29	12345.0
30	13495.0
31	14645.0
32	14645.0
33	14645.0
34	16768.5
35	18892.0
36	18892.0
37	18892.0
38	22794.5
39	26697.0
40	26697.0
41	26697.0
42	50900.0
43	75103.0
44	75103.0
45	75103.0
46	65442.0
47	55781.0
48	55781.0
49	55781.0
50	50150.5
51	44520.0
52	44520.0
53	44520.0
54	37524.0
55	30528.0
56	30528.0
57	30528.0
58	27382.5
59	24237.0
60	24237.0
61	24237.0
62	22037.0
63	19837.0
64	19837.0
65	19837.0
66	15680.0
67	11523.0
68	11523.0
69	11523.0
70	9806.0
71	8089.0
72	8089.0
73	8089.0
74	6495.0
75	4901.0
76	4901.0
77	4901.0
78	3702.0
79	2503.0
80	2503.0
81	2503.0
82	1797.5
83	1092.0
84	1092.0
85	1092.0
86	727.0
87	362.0
88	362.0
89	362.0
90	236.0
91	110.0
92	110.0
93	110.0
94	64.5
95	19.0
96	19.0
97	19.0
98	10.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13858752372492197
2	0.2732916842631561
3	0.3354254952501614
4	0.3638225885528162
5	0.37256015572286383
6	0.33057129126680157
7	0.3346973646526574
8	0.34974539700107277
9	0.3308140014659696
10	0.36867679253617597
11	0.28130112083569975
12	0.3286296096734577
13	0.3031450387608188
14	0.3096982141383545
15	0.3516870785944167
16	0.30581485095166666
17	0.3519297887935847
18	0.3201347527025781
19	0.33421194425432144
20	0.29562102258661116
21	0.3351827850509934
22	0.2866407452173955
23	0.27280626386482015
24	0.30921279374001853
25	0.29926167557413097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	412014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.525833500784668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40665949280937	26.29464460026949
2	6.687550130711024	4.216611838978929
3	2.354892825081626	2.2271987734714735
4	1.305645747807343	1.6464628182552714
5	0.9313209930174782	1.4680335280827228
6	0.6400037579670759	1.2105991148127913
7	0.5197133223796199	1.1469076968635665
8	0.4280575474522889	1.0795896775788074
9	0.33874370339089116	0.961125983328638
>10	2.8985473839434577	17.9940394617093
>50	0.2617545392906285	5.659193648935322
>100	0.1916967067157838	11.92175631666608
>500	0.016167192132656463	3.3350336634224065
>1k	0.016937058424687724	9.58206309816728
>5k	7.698662920312602E-4	1.5390035994295523
>10k+	0.0015397325840625204	9.717736180028362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	29162	7.077914828136908	No Hit
GTACATAAGCAGTGGTATCAACGCA	10877	2.639958836350221	No Hit
GCTTATGTACTCTGCGTTGATACCA	6341	1.539025372924221	No Hit
CTTATGTACTCTGCGTTGATACCAC	4504	1.0931667370526243	No Hit
GAGTACATAAGCAGTGGTATCAACG	3183	0.7725465639517104	No Hit
TATGTACTCTGCGTTGATACCACTG	2577	0.6254641832559088	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2451	0.5948826981607421	No Hit
GCGTTGATACCACTGCTTATGTACT	2227	0.5405156135471125	No Hit
CCCATGTACTCTGCGTTGATACCAC	1971	0.4783818025601072	No Hit
CATAAGCAGTGGTATCAACGCAGAG	1946	0.47231404758090745	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1768	0.42911163212900527	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1696	0.41163649778891015	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1683	0.40848126519972616	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1674	0.4062968734072143	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1551	0.37644351890955163	No Hit
GTATCAACGCAGAGTACATAAGCAG	1441	0.34974539700107277	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1427	0.34634745421272095	No Hit
GTGGTATCAACGCAGAGTACATGGG	1356	0.32911503007179366	No Hit
TATCAACGCAGAGTACATAAGCAGT	1305	0.3167368099142262	No Hit
GGTATCAACGCAGAGTACATAAGCA	1288	0.3126107365283704	No Hit
CATGTACTCTGCGTTGATACCACTG	1269	0.3079992427441786	No Hit
ATACAGGGTGACAGCCCCGTACACA	1114	0.2703791618731402	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1036	0.25144776633803706	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1011	0.24538001135883733	No Hit
GTGGTATCAACGCAGAGTACATAAG	1002	0.2431956195663254	No Hit
GGATACCACGTGTCCCGCCCTACTC	930	0.22572048522623017	No Hit
GTACATGGGAAGCAGTGGTATCAAC	892	0.21649749765784657	No Hit
ACATAAGCAGTGGTATCAACGCAGA	868	0.21067245287781483	No Hit
GATACCACTGCTTATGTACTCTGCG	752	0.18251806977432805	No Hit
ATCATGTACTCTGCGTTGATACCAC	712	0.17280966180760848	No Hit
CCACTGCTTATGTACTCTGCGTTGA	712	0.17280966180760848	No Hit
GTACATGATAAGCAGTGGTATCAAC	683	0.16577106603173677	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	677	0.16431480483672886	No Hit
GTACTCTGCGTTGATACCACTGCTT	674	0.16358667423922488	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	652	0.15824704985752913	No Hit
ATACCACTGCTTATGTACTCTGCGT	619	0.15023761328498544	No Hit
GCAGTGGTATCAACGCAGAGTACAT	614	0.1490240622891455	No Hit
GTTGATACCACTGCTTATGTACTCT	609	0.14781051129330558	No Hit
GGTATCAACGCAGAGTACATGGGGG	595	0.1444125685049537	No Hit
CCCATATTCAGACAGGATACCACGT	583	0.14150004611493786	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	570	0.13834481352575398	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	533	0.12936453615653837	No Hit
GTTAATGATAGTGTGTCGAAACACA	531	0.12887911575820238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	515	0.12499575257151456	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	510	0.12378220157567461	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	510	0.12378220157567461	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	498	0.12086967918565875	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	481	0.11674360579980292	No Hit
TACCACTGCTTATGTACTCTGCGTT	479	0.11625818540146693	No Hit
TGATACCACTGCTTATGTACTCTGC	474	0.11504463440562698	No Hit
CAGTGGTATCAACGCAGAGTACATA	456	0.11067585082060317	No Hit
GCAGAGTACATAAGCAGTGGTATCA	455	0.1104331406214352	No Hit
TATTCAGACAGGATACCACGTGTCC	451	0.10946229982476323	No Hit
ATCATTAACTGAATCCATAGGTTAA	444	0.10776332843058731	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	443	0.10752061823141933	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	440	0.10679248763391536	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	437	0.10606435703641139	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	436	0.1058216468372434	No Hit
GTATCAACGCAGAGTACATGGGGGT	423	0.10266641424805953	No Hit
GAGTACATGGGAAGCAGTGGTATCA	417	0.10121015305305159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.8542039833597886E-4	0.0
2	0.0	0.0	0.0	4.8542039833597886E-4	0.0
3	0.0	0.0	0.0	4.8542039833597886E-4	0.0
4	0.0	0.0	0.0	4.8542039833597886E-4	0.0
5	0.0	0.0	0.0	4.8542039833597886E-4	0.0
6	0.0	0.0	0.0	4.8542039833597886E-4	0.0
7	0.0	0.0	0.0	4.8542039833597886E-4	0.0
8	0.0	0.0	0.0	7.281305975039683E-4	0.0
9	0.0	0.0	0.0	7.281305975039683E-4	0.0
10	0.0	0.0	0.0	7.281305975039683E-4	0.0
11	0.0	0.0	0.0	7.281305975039683E-4	0.0
12	0.0	0.0	0.0	0.0012135509958399473	0.0
13	0.0	0.0	0.0	0.0014562611950079366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCTA	40	2.7582762E-4	16.622332	13
CCGGTTT	35	0.00216886	16.283102	14
TCGATGG	50	8.70093E-5	15.19756	12
CGGTTTA	45	6.755534E-4	14.775406	15
CTATTTA	45	6.7617855E-4	14.773604	17
GCTAAAA	40	0.00524613	14.259887	1
AAAAGTA	70	7.1797367E-6	13.580846	2
ACCGATA	50	0.0014980764	13.297866	19
TGTCTAT	50	0.0014980764	13.297866	14
TCCCGGT	50	0.0014980764	13.297866	12
TCTATTT	50	0.0014980764	13.297866	16
ACACCTT	50	0.0014994543	13.296244	6
GACTAAG	50	0.0015008333	13.294623	18
GAACATA	80	1.9737417E-6	13.071564	1
CGAATGG	80	1.992772E-6	13.060403	19
GATAAAA	60	4.0567972E-4	12.675456	1
CGATGGC	60	4.0861458E-4	12.664634	13
CCGAATG	75	1.4784426E-5	12.661546	18
CCTTCGA	60	4.0987806E-4	12.660002	9
GAACAAA	190	0.0	12.508674	1
>>END_MODULE