FastQCFastQC Report
Wed 25 May 2016
SRR1295241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412014
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118932.8865523987048984No Hit
GTACATAAGCAGTGGTATCAACGCA115622.806215322780294No Hit
GGTATCAACGCAGAGTACTTTTTTT81801.9853694291941535No Hit
TATCAACGCAGAGTACTTTTTTTTT78541.9062459042653892No Hit
GCTTATGTACTCTGCGTTGATACCA68601.6649919662924075No Hit
CTTATGTACTCTGCGTTGATACCAC49981.213065575441611No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47201.14559214007291No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44491.079817676098385No Hit
GAGTACATAAGCAGTGGTATCAACG32420.7868664657026218No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26390.6405122156043241No Hit
TATGTACTCTGCGTTGATACCACTG25490.6186682976792051No Hit
GCGTTGATACCACTGCTTATGTACT23920.5805627964098307No Hit
CATAAGCAGTGGTATCAACGCAGAG23600.572796070036455No Hit
CCCATGTACTCTGCGTTGATACCAC22750.5521657031071759No Hit
CTGCTTATGTACTCTGCGTTGATAC20120.48833292072599477No Hit
ACGCAGAGTACATAAGCAGTGGTAT18900.4587222764275No Hit
ACTCTGCGTTGATACCACTGCTTAT18160.44076172168906885No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16720.40581145300887833No Hit
GTATCAACGCAGAGTACATAAGCAG15290.37110389452785586No Hit
GTACTGGTTCACTATCGGTCAGTCA15170.36819137213784No Hit
TATCAACGCAGAGTACATAAGCAGT14990.3638225885528162No Hit
GTGGTATCAACGCAGAGTACATGGG14650.35557044178110453No Hit
CATGTACTCTGCGTTGATACCACTG14510.35217249899275266No Hit
GGTATCAACGCAGAGTACATAAGCA13660.33154213206347355No Hit
ATCAACGCAGAGTACTTTTTTTTTT12920.3135815773250423No Hit
AAGCAGTGGTATCAACGCAGAGTAC12150.2948928919891072No Hit
ATACAGGGTGACAGCCCCGTACACA11720.2844563534248836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11630.2822719616323717No Hit
GTGGTATCAACGCAGAGTACTTTTT10840.26309785589810053No Hit
ACATAAGCAGTGGTATCAACGCAGA10390.25217589693554104No Hit
GTGGTATCAACGCAGAGTACATAAG10120.2456227215580053No Hit
GGATACCACGTGTCCCGCCCTACTC9830.2385841257821336No Hit
GTACATGGGAAGCAGTGGTATCAAC8700.2111578732761508No Hit
ATCATGTACTCTGCGTTGATACCAC7770.18858582475352778No Hit
GTACATGATAAGCAGTGGTATCAAC7590.184217041168504No Hit
CCACTGCTTATGTACTCTGCGTTGA7550.18324620037183204No Hit
GATACCACTGCTTATGTACTCTGCG7240.17572218419762434No Hit
GTACTCTGCGTTGATACCACTGCTT6930.1681981680234167No Hit
ATACCACTGCTTATGTACTCTGCGT6740.16358667423922488No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6670.16188770284504894No Hit
GCAGTGGTATCAACGCAGAGTACAT6540.1587324702558651No Hit
CCCATATTCAGACAGGATACCACGT6520.15824704985752913No Hit
GGTATCAACGCAGAGTACATGGGGG6310.15315013567500135No Hit
GTTGATACCACTGCTTATGTACTCT6290.15266471527666536No Hit
CAACGCAGAGTACTTTTTTTTTTTT6200.15048032348415347No Hit
AACGCAGAGTACTTTTTTTTTTTTT5920.14368443790744975No Hit
GTTAATGATAGTGTGTCGAAACACA5850.14198546651327382No Hit
CAGTGGTATCAACGCAGAGTACATA5530.13421874013989815No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5460.13251976874572224No Hit
TACCACTGCTTATGTACTCTGCGTT5240.12718014436402647No Hit
CTATCGGTCAGTCAGGAGTATTTAG5170.12548117296985054No Hit
GCCTTGGAGGATGGTCCCCCCATAT5100.12378220157567461No Hit
GTATTTAGCCTTGGAGGATGGTCCC5080.12329678117733864No Hit
GGTTAATGAGGCGAACCGGGGGAAC5020.12184051998233068No Hit
GTTCACTATCGGTCAGTCAGGAGTA5010.1215978097831627No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4930.1196561281898188No Hit
ATCATTAACTGAATCCATAGGTTAA4690.11383108340978704No Hit
GCAGAGTACATAAGCAGTGGTATCA4630.11237482221477912No Hit
CACACACACTGATTCAGGCTCTGGG4600.11164669161727514No Hit
TGATACCACTGCTTATGTACTCTGC4570.11091856101977117No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4480.10873416922725927No Hit
CGGTACTGGTTCACTATCGGTCAGT4470.10849145902809129No Hit
GGTATCAACGCAGAGTACATGGGGA4440.10776332843058731No Hit
GTATCAACGCAGAGTACATGGGGGT4380.10630706723557938No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4320.10485080604057144No Hit
TCAACGCAGAGTACTTTTTTTTTTT4250.10315183464639552No Hit
TATTCAGACAGGATACCACGTGTCC4240.10290912444722754No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4230.10266641424805953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTT250.00602205119.0014558
TTAACAC359.911256E-519.0014553
CAGTCCC250.00602205119.0014554
CCAGATG504.5425822E-617.101313
CTTAACA402.7533303E-416.6262742
TCTTAGA402.7533303E-416.6262742
TACAGTT350.00216545116.2869637
GTGTAAG551.1221886E-515.54853251
AAAAGTA551.123481E-515.5466452
CACAATC508.701511E-515.19747617
TAACACC456.743495E-414.778914
CTAGAGT400.005267712314.2510923
CGCAAAA400.005267712314.2510922
TCGACGG400.005267712314.25109211
AACACCT400.005267712314.2510925
ACAATCT400.005276205514.24763318
TTTTGGA551.9533833E-413.8192412
ACAGGGT1900.013.0009973
ACTATCC604.078053E-412.66763810
AATCGCC604.086416E-412.66456318
Produced by FastQC (version 0.11.5)