##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295241_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412014
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.869356381093848	31.0	30.0	33.0	28.0	34.0
2	30.818484323348237	31.0	30.0	33.0	27.0	34.0
3	31.02591902216915	31.0	31.0	34.0	28.0	34.0
4	34.55304431402816	37.0	35.0	37.0	32.0	37.0
5	34.88279524482178	37.0	35.0	37.0	32.0	37.0
6	34.635354623872004	37.0	35.0	37.0	32.0	37.0
7	34.57124029765979	37.0	35.0	37.0	31.0	37.0
8	34.618673151883186	37.0	35.0	37.0	31.0	37.0
9	36.1170639832627	39.0	37.0	39.0	31.0	39.0
10	36.147130922735634	39.0	37.0	39.0	31.0	39.0
11	36.22535641992748	39.0	37.0	39.0	31.0	39.0
12	36.160853757396595	39.0	37.0	39.0	31.0	39.0
13	35.94495575393069	39.0	37.0	39.0	30.0	39.0
14	36.99121389079012	40.0	37.0	41.0	30.0	41.0
15	36.972932958588785	40.0	37.0	41.0	30.0	41.0
16	36.85271859694088	40.0	37.0	41.0	30.0	41.0
17	37.06577446397453	40.0	37.0	41.0	31.0	41.0
18	37.10254020494449	40.0	37.0	41.0	30.0	41.0
19	37.24494798720432	40.0	37.0	41.0	31.0	41.0
20	37.256399054401065	40.0	37.0	41.0	30.0	41.0
21	37.21801929060663	40.0	37.0	41.0	30.0	41.0
22	37.18432140655415	40.0	37.0	41.0	30.0	41.0
23	37.09869810249166	40.0	37.0	41.0	30.0	41.0
24	37.10582164683724	40.0	37.0	41.0	30.0	41.0
25	35.93139068089919	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1451929285812561
1101	2	-0.08717248018285417
1101	3	-0.22271059526195458
1101	4	-0.3195560599051106
1101	5	-0.1438749707761744
1101	6	-0.10141752891244948
1101	7	-0.2037371218922317
1101	8	-0.15587000409197316
1101	9	-0.11487635721933032
1101	10	0.08726662541886299
1101	11	0.07543734290870674
1101	12	-0.004912510555747929
1101	13	0.18790522123288156
1101	14	0.17050627635367732
1101	15	0.13026811097930135
1101	16	0.1452214381563266
1101	17	0.06907486292660536
1101	18	-0.15958198838443138
1101	19	0.29458325656159445
1101	20	0.2760671225579472
1101	21	-0.04550023159804795
1101	22	0.04367441046553466
1101	23	-0.032330792519509544
1101	24	-0.006612654155610187
1101	25	0.1753389804781733
1102	1	-0.09423223326410479
1102	2	0.12512323860079277
1102	3	0.16015908729287176
1102	4	-0.08678110658822646
1102	5	-0.15873405819512953
1102	6	0.021850879681785784
1102	7	0.10900112100534187
1102	8	0.03115322615232685
1102	9	0.09182647466239757
1102	10	0.08404310530763581
1102	11	0.39032005584504503
1102	12	0.2068902527211094
1102	13	0.4338877474367635
1102	14	0.40649566304433193
1102	15	0.3738608252332085
1102	16	0.1250193491153908
1102	17	0.32265617332501506
1102	18	0.2648118126432806
1102	19	0.2233568650413389
1102	20	0.10180167169675514
1102	21	0.4861079846299532
1102	22	0.18753790949552496
1102	23	0.18241607369191115
1102	24	0.1462489866365715
1102	25	0.45024686367725053
1103	1	-0.2720133735063861
1103	2	-0.2400106027703579
1103	3	-0.24443938859367265
1103	4	-0.1980221311005934
1103	5	-0.269133702385254
1103	6	-0.21488649044302122
1103	7	-0.007324866871442737
1103	8	0.035330324100911525
1103	9	0.13948711640402678
1103	10	0.2669848643656607
1103	11	0.010749778808538224
1103	12	0.10507406337156056
1103	13	0.19370920057164653
1103	14	0.25970574676969704
1103	15	0.1403376654947479
1103	16	-0.12972401842338144
1103	17	0.21284572461940598
1103	18	0.34118255186582047
1103	19	0.2134207824347527
1103	20	0.21785484143090628
1103	21	0.05379210248739241
1103	22	0.13716742704301055
1103	23	0.30843971487415445
1103	24	0.16424810628222986
1103	25	-0.12283171711818852
1104	1	0.04293883080235972
1104	2	-0.1950422287750193
1104	3	-0.023530557091319793
1104	4	-0.31539027681800036
1104	5	0.17300022066606147
1104	6	-0.17499967824917917
1104	7	-0.15960526826013677
1104	8	0.04532223925761514
1104	9	-0.027024987081965435
1104	10	-0.015758750986030634
1104	11	0.02574954101902449
1104	12	-0.011825637013657797
1104	13	-0.026514797145573255
1104	14	0.08912506478938553
1104	15	0.08410520247101516
1104	16	-0.25721724142238145
1104	17	-0.23232001466817565
1104	18	-0.032584509531432104
1104	19	0.022765910127724
1104	20	0.1080032220843492
1104	21	-0.16239898516182194
1104	22	0.08565032997732658
1104	23	-0.23171817735964595
1104	24	-0.06883079227676348
1104	25	0.42229122005782216
1105	1	-0.30268822013215413
1105	2	-0.2584155107458166
1105	3	-0.3103903088390716
1105	4	-0.10906507588586578
1105	5	-0.07434842631163008
1105	6	-0.07224845363320753
1105	7	-0.02419603251561142
1105	8	-0.016816915162891632
1105	9	0.13335214707887388
1105	10	0.22250633675830045
1105	11	0.05522830641589849
1105	12	0.20016608791144108
1105	13	0.03726748278023706
1105	14	0.3103192437022102
1105	15	0.08358919923708186
1105	16	0.12334346623919856
1105	17	0.22511566326971888
1105	18	-0.1848910677660811
1105	19	0.20115084378444692
1105	20	0.10589165124685707
1105	21	0.15041786935856294
1105	22	0.15710607734975923
1105	23	-0.02131488635284029
1105	24	0.2486039345030875
1105	25	-0.1458378520875101
1106	1	-0.4280978135500746
1106	2	-0.6457071412593791
1106	3	-0.48408854184541283
1106	4	-0.3548619583823225
1106	5	-0.21184931950600117
1106	6	0.006191875878954534
1106	7	0.050135602897753984
1106	8	0.18072705551991675
1106	9	-0.032468111590212345
1106	10	0.1374742328006846
1106	11	-0.042157951661131676
1106	12	0.14942499178331303
1106	13	-0.016005181388891287
1106	14	-0.04413345782795375
1106	15	-0.16029624730906278
1106	16	-0.07526468173503531
1106	17	-0.3235761897768441
1106	18	-0.33057106939935466
1106	19	-0.07766360656925997
1106	20	-0.1699676017364169
1106	21	-0.3033759099254567
1106	22	-0.23403649688745531
1106	23	-0.2723765116603545
1106	24	0.006384933451670349
1106	25	0.4143251686448082
1107	1	0.03684966058080974
1107	2	0.1405662295525829
1107	3	-0.045933199171958705
1107	4	3.8422893861422835E-4
1107	5	-0.00629201363511811
1107	6	0.055306271019205155
1107	7	0.045993098787249664
1107	8	0.23911839539724156
1107	9	0.22900684632017487
1107	10	-0.07032046833844419
1107	11	0.12059840116305764
1107	12	0.1516943850036796
1107	13	-0.004924044427809804
1107	14	-0.028420001950671292
1107	15	0.09669407454110512
1107	16	0.050241132046728865
1107	17	-0.11319703865790132
1107	18	-0.10410187112034919
1107	19	0.10486994205286493
1107	20	0.0732020661740691
1107	21	-0.27658870122989043
1107	22	0.1385378122029124
1107	23	0.014197147625452544
1107	24	0.20610384374910495
1107	25	0.6061405847658676
1108	1	0.08186851936484985
1108	2	0.0373187123374521
1108	3	0.07269547310043833
1108	4	-0.021857025084017323
1108	5	-0.1069875394318558
1108	6	-0.016737744344645478
1108	7	-0.17000918502880324
1108	8	0.053869770877362555
1108	9	-0.19137967224448715
1108	10	-0.4450393167415072
1108	11	-0.3103853699016881
1108	12	-0.1980149474991748
1108	13	-0.19717983182529508
1108	14	-0.06490050300607209
1108	15	-0.3250679241439727
1108	16	0.04601953268343095
1108	17	-0.2674757334224225
1108	18	0.09505726933433323
1108	19	0.19172415846173152
1108	20	0.13542740269090814
1108	21	0.2012825053862599
1108	22	-0.08081233832132284
1108	23	-0.043720081344410744
1108	24	-0.020850376120094438
1108	25	0.10871852863935771
1109	1	0.1136073921304721
1109	2	0.012046577703799244
1109	3	0.24454555284525625
1109	4	-0.022517207581991272
1109	5	0.1416920955721963
1109	6	0.33145949988365686
1109	7	0.04296176694128917
1109	8	0.08292433724858483
1109	9	0.11811301127753637
1109	10	0.2786993642879452
1109	11	0.14257390359253463
1109	12	0.046442134102001376
1109	13	0.2822433909111268
1109	14	-0.004619086598687261
1109	15	-0.01313714090171203
1109	16	0.1505462493758074
1109	17	0.18889557238805565
1109	18	0.07005530839622054
1109	19	-0.019392617581701188
1109	20	0.15296908351989913
1109	21	0.08028172972629477
1109	22	0.04803436860994026
1109	23	0.047557230165857334
1109	24	-0.1956876182567342
1109	25	-0.17679841822357645
1110	1	-0.16819221605430812
1110	2	-0.19636948937355214
1110	3	-0.04895323323513168
1110	4	-0.3303972926669232
1110	5	0.04670343875545058
1110	6	-0.04807722063180364
1110	7	-0.013077538404822064
1110	8	-0.12041051440557737
1110	9	-0.029693309949031743
1110	10	0.09299840863027953
1110	11	0.0400319691928388
1110	12	0.1525108477009951
1110	13	-0.1564437185450629
1110	14	-0.09520313892326016
1110	15	-0.045559186920101524
1110	16	-0.07628424853023574
1110	17	-0.052541761499540485
1110	18	-0.40240485779357016
1110	19	-0.16123105350487776
1110	20	-0.17663551920334442
1110	21	-0.04397145543072156
1110	22	-0.08325910806467363
1110	23	0.05596843272017793
1110	24	-0.04084100551256853
1110	25	-0.2053443238429864
1111	1	-0.09628643409374504
1111	2	0.020743744345480764
1111	3	-0.03114029976367405
1111	4	-0.005532930824699633
1111	5	0.06027603370215928
1111	6	0.0789749100724606
1111	7	-0.16702465269204225
1111	8	-0.034550243656006785
1111	9	-0.06093398208219725
1111	10	-0.1975394032496851
1111	11	0.06290609347075105
1111	12	-0.195938751090992
1111	13	-0.027113393540233233
1111	14	0.1150711223096863
1111	15	-0.24502190179025263
1111	16	-0.03555768452132213
1111	17	-0.015563902563222598
1111	18	0.22725907562521286
1111	19	0.12410071128363143
1111	20	-0.03774467938806225
1111	21	-0.10203248821213862
1111	22	-0.22092964341838695
1111	23	-0.13964900622070786
1111	24	-0.17703225092544983
1111	25	-0.33616710754182577
1112	1	0.06018199746326758
1112	2	0.15372817333516764
1112	3	0.12486434252511458
1112	4	0.03393531609582823
1112	5	0.0792628190050948
1112	6	-0.0739964185189379
1112	7	-0.11926754638538739
1112	8	0.16410542688215912
1112	9	0.03725965763047867
1112	10	0.03534875100091028
1112	11	-0.13341744240587872
1112	12	0.059310200437536764
1112	13	0.1404156427452321
1112	14	0.20632330937282006
1112	15	-0.12443229594519067
1112	16	-0.189606059280635
1112	17	0.17182894233333457
1112	18	0.24346971947901608
1112	19	-0.16263095914903403
1112	20	0.11616521800680601
1112	21	0.2028704897106124
1112	22	0.4086170300475942
1112	23	0.29982979135616006
1112	24	0.4376621756903347
1112	25	0.21325612324498877
1113	1	0.26096208661631337
1113	2	0.14231527236497143
1113	3	0.12475945658857057
1113	4	0.3899334805919352
1113	5	0.0915934719200564
1113	6	-0.027986454354966384
1113	7	-0.05308452042611833
1113	8	-0.1202272363465795
1113	9	-0.25374637331087513
1113	10	0.1573944637882505
1113	11	0.16119185748049603
1113	12	0.11478175012724279
1113	13	0.12779436838110314
1113	14	0.15690892251852517
1113	15	-0.13895681303285556
1113	16	0.2540002688375367
1113	17	0.0033275001517054648
1113	18	0.11371052010832727
1113	19	0.21325838618790982
1113	20	0.12582127953989186
1113	21	0.14904652327411583
1113	22	0.25174700120395954
1113	23	-9.612234152740484E-4
1113	24	0.26328275081488783
1113	25	-0.4940334493448191
1114	1	0.3377164725812243
1114	2	0.15281578836084364
1114	3	0.23936213976710619
1114	4	0.4781429749159827
1114	5	0.17956216817048443
1114	6	0.11191722641558499
1114	7	0.1545522184799708
1114	8	0.2570861451463742
1114	9	-0.14021008160121085
1114	10	-0.04153054481167828
1114	11	-0.1627245511882336
1114	12	-0.0021085147506312296
1114	13	-0.0846067324100872
1114	14	-0.17747360242127286
1114	15	-0.00781646215566667
1114	16	-0.028541870825343096
1114	17	-0.09642310184347735
1114	18	-0.08769126867736077
1114	19	-0.1284512801347546
1114	20	-0.2695433575430144
1114	21	0.028767885503214075
1114	22	-0.13636789387687998
1114	23	-0.23231657257247917
1114	24	-0.25769248138325196
1114	25	0.10287057542298328
1115	1	0.006444014327158243
1115	2	0.03880712211692128
1115	3	0.21730020782882775
1115	4	0.5806847208470742
1115	5	0.35110800062309977
1115	6	0.11241042728925521
1115	7	-0.0411780710993952
1115	8	-0.18247032635366622
1115	9	-0.2742814548133552
1115	10	-0.4431193561975775
1115	11	-0.0011043863182322866
1115	12	-0.08881498977353175
1115	13	-0.17472157775638664
1115	14	-0.2258160951994057
1115	15	0.019476921906900202
1115	16	-0.10889838459974044
1115	17	-0.14288086271150746
1115	18	-0.1904531935699154
1115	19	-0.015781301976510065
1115	20	-0.130628047178746
1115	21	-0.4005282463927884
1115	22	-0.3794542744870384
1115	23	-0.47236448351449667
1115	24	-0.41384340085059534
1115	25	-0.5484452140507017
1116	1	0.3018535594845311
1116	2	0.3708492440932005
1116	3	0.2958492878699204
1116	4	0.6753073976005979
1116	5	0.5652482826815657
1116	6	0.3164731899678799
1116	7	-0.08518666360897242
1116	8	0.29028347956769807
1116	9	-0.07818777777963959
1116	10	-0.15994479749765844
1116	11	-0.13238999386469175
1116	12	-0.1560116835252856
1116	13	-0.2451194483083654
1116	14	0.01935762292892207
1116	15	-0.12364673551348204
1116	16	1.4709966288961596E-4
1116	17	-0.223017689086781
1116	18	-0.17211939325236614
1116	19	-0.1431255627404937
1116	20	-0.16796577016371117
1116	21	-0.3546470471773233
1116	22	-0.498845794053679
1116	23	-0.25497399131312903
1116	24	-0.4409238155325781
1116	25	-1.7963134311049629
1201	1	0.1285436889902023
1201	2	0.13004000617540967
1201	3	0.04451857685388916
1201	4	0.06426146104227826
1201	5	-0.07199092326235501
1201	6	-0.022854668703018888
1201	7	0.061106437856302875
1201	8	0.16297227491192956
1201	9	0.2729773131963711
1201	10	0.2087254115976407
1201	11	0.14537321548363735
1201	12	0.18515179725589093
1201	13	0.27679070281992324
1201	14	0.45524196493430225
1201	15	0.36987108014221803
1201	16	0.26496204227094466
1201	17	0.13440185867850118
1201	18	0.09907882173165206
1201	19	0.2438134568808792
1201	20	0.21357219593018328
1201	21	0.13703744664643125
1201	22	0.30546492754363186
1201	23	-0.030084979742731832
1201	24	0.1713251665942579
1201	25	0.29762303520745803
1202	1	-0.01601359727426299
1202	2	-0.07957369598832642
1202	3	-0.06009661349903794
1202	4	-0.34235241248870096
1202	5	-0.4159114449403063
1202	6	-0.06494336477871343
1202	7	-0.051761438001655335
1202	8	0.0675342512271726
1202	9	0.08269203183841967
1202	10	-0.02063611014344957
1202	11	0.15902396904852623
1202	12	0.0847531429396895
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2210	23	0.4745875035381957
2210	24	0.622040432992236
2210	25	0.7675995620687033
2211	1	0.2864836961543169
2211	2	0.25645790408233893
2211	3	0.3786737197603074
2211	4	0.5154857730439417
2211	5	0.26389775876146615
2211	6	0.6056770855766516
2211	7	0.3961659398175925
2211	8	0.45401226571355835
2211	9	0.31483432275294376
2211	10	0.5231106906664849
2211	11	0.4212624281826933
2211	12	0.41454943663553223
2211	13	0.3493074743686577
2211	14	0.18912159894527747
2211	15	0.22531293361393523
2211	16	0.3234225533996522
2211	17	0.3796556320005706
2211	18	0.5752714037762559
2211	19	0.5007266074555545
2211	20	0.45103167666446353
2211	21	0.5065112379230143
2211	22	0.5115217009497286
2211	23	0.5989730274832965
2211	24	0.4496178959012411
2211	25	-0.038199312411357766
2212	1	-0.01923557077753557
2212	2	-0.0346440765283198
2212	3	-0.0823544870855315
2212	4	0.0886911242057522
2212	5	0.08153189238340985
2212	6	0.21545510967914083
2212	7	0.13508187351066425
2212	8	0.029936401582972394
2212	9	-0.09782526401796332
2212	10	0.014204816040425783
2212	11	-0.12602500583876974
2212	12	0.0229593403434194
2212	13	-0.03942637057333798
2212	14	-0.08985276898091854
2212	15	-0.023986095609721758
2212	16	-0.1459911186398557
2212	17	-0.10234169285492101
2212	18	-0.040856270004496764
2212	19	0.1806126192427726
2212	20	0.02761914363428275
2212	21	-0.05293435692569659
2212	22	-0.1232207018362601
2212	23	0.09100759925288315
2212	24	-0.0705536973807881
2212	25	0.11462213587608971
2213	1	0.14036737295992552
2213	2	0.17762450619066072
2213	3	0.19355102607980257
2213	4	0.44145157681720804
2213	5	0.43526843089042444
2213	6	0.3133109456623089
2213	7	0.24868623192768524
2213	8	0.40770314285356335
2213	9	0.05486552960007174
2213	10	0.27154104753188335
2213	11	0.19292503533313266
2213	12	0.10855820343984846
2213	13	0.2671914487991742
2213	14	0.3989362180182212
2213	15	0.6748097067909313
2213	16	0.2920844011688857
2213	17	0.3732873343056369
2213	18	0.4247388521459925
2213	19	0.17297592959293695
2213	20	0.15025982147648875
2213	21	0.282207100280246
2213	22	0.4391660216755824
2213	23	0.3396235567523007
2213	24	0.3068410992644033
2213	25	0.1954975172268334
2214	1	0.25649780090202867
2214	2	0.37837969813528005
2214	3	0.20842542297512523
2214	4	0.09131653381322735
2214	5	0.10299053074358255
2214	6	0.1797691478859278
2214	7	-0.03827359605637071
2214	8	-0.032674715338174565
2214	9	-0.035469062386503936
2214	10	-0.05223438775236389
2214	11	-0.17047831058673069
2214	12	-0.03267973446659056
2214	13	-0.04993672405586835
2214	14	-0.20553855647307273
2214	15	-0.1678258606519094
2214	16	-0.1886818039915923
2214	17	-0.0465849648342882
2214	18	-0.0983167908160425
2214	19	-0.09111836451236854
2214	20	0.025908814553893933
2214	21	-0.10907672602560581
2214	22	0.04332613285662035
2214	23	0.08757168974999274
2214	24	0.055982330646820344
2214	25	-0.05886188071736598
2215	1	0.18528944087640298
2215	2	0.28655821617926946
2215	3	0.13026372107463402
2215	4	0.1493922865358428
2215	5	0.14769344554179042
2215	6	-0.12762595140939226
2215	7	0.14338079215631439
2215	8	0.18959866710897444
2215	9	-0.2504754685364077
2215	10	-0.3643873879659054
2215	11	0.0181227464280056
2215	12	-0.014335457293839227
2215	13	-0.15505096706249333
2215	14	-0.27362391842054024
2215	15	0.022590272168933723
2215	16	-0.030436555219438333
2215	17	-0.01067716000832064
2215	18	-0.031152312917257063
2215	19	-0.32970740999603265
2215	20	-0.19266768555287683
2215	21	0.051130406154619834
2215	22	0.13073671368088213
2215	23	-0.04777824618850701
2215	24	0.28974674448410553
2215	25	0.08402862750385509
2216	1	0.33688233201508666
2216	2	0.22471332361001117
2216	3	0.23408821876828867
2216	4	0.49522940264174053
2216	5	0.3458969724613752
2216	6	-0.01089876038167148
2216	7	-0.37695676871193484
2216	8	-0.3250991237518477
2216	9	-0.44640245414812085
2216	10	-0.30203354054231824
2216	11	-0.5797410187374794
2216	12	-0.3550486360149421
2216	13	-0.3768429466676153
2216	14	-0.6359997747026966
2216	15	-0.5007666289837758
2216	16	-0.4364724846810475
2216	17	-0.2932892447057469
2216	18	-0.25237573034277005
2216	19	-0.4215485427186181
2216	20	-0.5413476125568266
2216	21	-0.2866986950586181
2216	22	-0.514673063754536
2216	23	-0.5780633526105134
2216	24	-0.3710279673374046
2216	25	-0.8053470545414996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	96.0
9	256.0
10	439.0
11	672.0
12	779.0
13	1016.0
14	1287.0
15	1570.0
16	1980.0
17	2262.0
18	2352.0
19	2390.0
20	2292.0
21	2218.0
22	2094.0
23	2096.0
24	2259.0
25	2351.0
26	2476.0
27	2800.0
28	3109.0
29	3764.0
30	4938.0
31	6556.0
32	9207.0
33	12721.0
34	18205.0
35	27375.0
36	43455.0
37	78263.0
38	170730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.82491539989027	17.261578198660963	13.89359563817856	21.0199107632702
2	14.798319470502145	22.559239635642562	38.57718081711014	24.06526007674515
3	20.260965173090877	28.785050811996825	31.092767391003594	19.8612166239087
4	12.895678302193614	18.9911022440985	33.14814545136816	34.965074002339726
5	11.845921941486456	40.04121629330078	32.24288970393691	15.869972061275861
6	30.031577439970096	33.298058509282704	20.003495120181164	16.66686893056604
7	23.52420063395904	36.164062386229595	20.51483687447514	19.796900105336228
8	24.702067445619726	32.99782042009097	22.422974422702584	19.877137711586723
9	28.06952393434983	17.320061553099254	19.281702516007204	35.3287119965437
10	17.830834613638956	27.36456065493397	27.96405921201133	26.84054551941574
11	29.717862708071397	26.561536706488866	20.378496982995063	23.342103602444674
12	26.162845734674807	27.14904668859303	25.920603905481997	20.767503671250168
13	30.60242601668645	20.718483508961015	29.015344057909797	19.66374641644273
14	27.41505614377467	23.76534669327003	26.66841433280418	22.151182830151125
15	29.908207002674665	23.249209978301707	26.731373205764854	20.111209813258775
16	22.328124772459187	25.252297252035127	29.725203512502002	22.694374463003683
17	19.88869283250771	28.60128199838355	28.30590296908107	23.20412220002767
18	19.948933526207973	21.638204712484104	37.47051056765337	20.942351193654556
19	21.767084453850856	27.211100647553955	31.118511111326853	19.90330378726833
20	21.254632787147855	24.149715176124793	35.121805591625424	19.473846445101927
21	22.41506942742098	25.843116600689786	31.255081757129023	20.486732214760213
22	20.710946715402876	24.776827971865035	30.62250311882606	23.88972219390603
23	23.57492706558515	21.74586300465518	31.389467348196902	23.289742581562763
24	20.735217735319676	24.366405025071963	30.266447256646618	24.631929982961744
25	22.65077377138308	25.953622259761943	30.904982427525677	20.490621541329297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1584.0
1	1584.0
2	1438.0
3	1292.0
4	1292.0
5	1292.0
6	3651.0
7	6010.0
8	6010.0
9	6010.0
10	5087.5
11	4165.0
12	4165.0
13	4165.0
14	3083.0
15	2001.0
16	2001.0
17	2001.0
18	3476.0
19	4951.0
20	4951.0
21	4951.0
22	8131.5
23	11312.0
24	11312.0
25	11312.0
26	15388.5
27	19465.0
28	19465.0
29	19465.0
30	21280.5
31	23096.0
32	23096.0
33	23096.0
34	23994.5
35	24893.0
36	24893.0
37	24893.0
38	26044.5
39	27196.0
40	27196.0
41	27196.0
42	52648.0
43	78100.0
44	78100.0
45	78100.0
46	67541.5
47	56983.0
48	56983.0
49	56983.0
50	51236.0
51	45489.0
52	45489.0
53	45489.0
54	38289.0
55	31089.0
56	31089.0
57	31089.0
58	28098.0
59	25107.0
60	25107.0
61	25107.0
62	22873.5
63	20640.0
64	20640.0
65	20640.0
66	16061.0
67	11482.0
68	11482.0
69	11482.0
70	9902.0
71	8322.0
72	8322.0
73	8322.0
74	6584.5
75	4847.0
76	4847.0
77	4847.0
78	3668.0
79	2489.0
80	2489.0
81	2489.0
82	1750.5
83	1012.0
84	1012.0
85	1012.0
86	689.0
87	366.0
88	366.0
89	366.0
90	235.5
91	105.0
92	105.0
93	105.0
94	60.5
95	16.0
96	16.0
97	16.0
98	9.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018445975136767198
2	2.4271019916798943E-4
3	0.0012135509958399473
4	0.0
5	0.009951118165887568
6	0.0026698121908478836
7	0.0
8	0.0019416815933439155
9	0.002912522390015873
10	7.281305975039683E-4
11	0.003883363186687831
12	0.007038595775871694
13	0.015290742547583334
14	0.00873756717004762
15	0.0
16	0.0
17	2.4271019916798943E-4
18	4.8542039833597886E-4
19	4.8542039833597886E-4
20	0.0012135509958399473
21	2.4271019916798943E-4
22	0.0
23	0.0
24	0.0
25	0.0014562611950079366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	412014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.147817839672076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0363962546659	20.12740713556151
2	7.6756849889334235	3.8605345579280623
3	2.6897332410155856	2.029227647471122
4	1.5959632537260546	1.6053997273405267
5	1.0632294756524474	1.3368950587738901
6	0.8655095541449032	1.3059405963679092
7	0.6428875856460408	1.131705392664925
8	0.54705274982266	1.1005746320985563
9	0.44722552671964666	1.0122071471277365
>10	3.7714687272584992	18.855621115651054
>50	0.3603759157834037	6.31876317557946
>100	0.24956780871426854	12.296860414358708
>500	0.02395850963656978	3.8725959772144916
>1k	0.025955052106283925	13.510655406568747
>5k	0.0029948137045712224	5.74738832505825
>10k+	0.0019965424697141482	5.88822369023505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11893	2.8865523987048984	No Hit
GTACATAAGCAGTGGTATCAACGCA	11562	2.806215322780294	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8180	1.9853694291941535	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7854	1.9062459042653892	No Hit
GCTTATGTACTCTGCGTTGATACCA	6860	1.6649919662924075	No Hit
CTTATGTACTCTGCGTTGATACCAC	4998	1.213065575441611	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4720	1.14559214007291	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4449	1.079817676098385	No Hit
GAGTACATAAGCAGTGGTATCAACG	3242	0.7868664657026218	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2639	0.6405122156043241	No Hit
TATGTACTCTGCGTTGATACCACTG	2549	0.6186682976792051	No Hit
GCGTTGATACCACTGCTTATGTACT	2392	0.5805627964098307	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2360	0.572796070036455	No Hit
CCCATGTACTCTGCGTTGATACCAC	2275	0.5521657031071759	No Hit
CTGCTTATGTACTCTGCGTTGATAC	2012	0.48833292072599477	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1890	0.4587222764275	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1816	0.44076172168906885	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1672	0.40581145300887833	No Hit
GTATCAACGCAGAGTACATAAGCAG	1529	0.37110389452785586	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1517	0.36819137213784	No Hit
TATCAACGCAGAGTACATAAGCAGT	1499	0.3638225885528162	No Hit
GTGGTATCAACGCAGAGTACATGGG	1465	0.35557044178110453	No Hit
CATGTACTCTGCGTTGATACCACTG	1451	0.35217249899275266	No Hit
GGTATCAACGCAGAGTACATAAGCA	1366	0.33154213206347355	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1292	0.3135815773250423	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1215	0.2948928919891072	No Hit
ATACAGGGTGACAGCCCCGTACACA	1172	0.2844563534248836	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1163	0.2822719616323717	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1084	0.26309785589810053	No Hit
ACATAAGCAGTGGTATCAACGCAGA	1039	0.25217589693554104	No Hit
GTGGTATCAACGCAGAGTACATAAG	1012	0.2456227215580053	No Hit
GGATACCACGTGTCCCGCCCTACTC	983	0.2385841257821336	No Hit
GTACATGGGAAGCAGTGGTATCAAC	870	0.2111578732761508	No Hit
ATCATGTACTCTGCGTTGATACCAC	777	0.18858582475352778	No Hit
GTACATGATAAGCAGTGGTATCAAC	759	0.184217041168504	No Hit
CCACTGCTTATGTACTCTGCGTTGA	755	0.18324620037183204	No Hit
GATACCACTGCTTATGTACTCTGCG	724	0.17572218419762434	No Hit
GTACTCTGCGTTGATACCACTGCTT	693	0.1681981680234167	No Hit
ATACCACTGCTTATGTACTCTGCGT	674	0.16358667423922488	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	667	0.16188770284504894	No Hit
GCAGTGGTATCAACGCAGAGTACAT	654	0.1587324702558651	No Hit
CCCATATTCAGACAGGATACCACGT	652	0.15824704985752913	No Hit
GGTATCAACGCAGAGTACATGGGGG	631	0.15315013567500135	No Hit
GTTGATACCACTGCTTATGTACTCT	629	0.15266471527666536	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	620	0.15048032348415347	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	592	0.14368443790744975	No Hit
GTTAATGATAGTGTGTCGAAACACA	585	0.14198546651327382	No Hit
CAGTGGTATCAACGCAGAGTACATA	553	0.13421874013989815	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	546	0.13251976874572224	No Hit
TACCACTGCTTATGTACTCTGCGTT	524	0.12718014436402647	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	517	0.12548117296985054	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	510	0.12378220157567461	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	508	0.12329678117733864	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	502	0.12184051998233068	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	501	0.1215978097831627	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	493	0.1196561281898188	No Hit
ATCATTAACTGAATCCATAGGTTAA	469	0.11383108340978704	No Hit
GCAGAGTACATAAGCAGTGGTATCA	463	0.11237482221477912	No Hit
CACACACACTGATTCAGGCTCTGGG	460	0.11164669161727514	No Hit
TGATACCACTGCTTATGTACTCTGC	457	0.11091856101977117	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	448	0.10873416922725927	No Hit
CGGTACTGGTTCACTATCGGTCAGT	447	0.10849145902809129	No Hit
GGTATCAACGCAGAGTACATGGGGA	444	0.10776332843058731	No Hit
GTATCAACGCAGAGTACATGGGGGT	438	0.10630706723557938	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	432	0.10485080604057144	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	425	0.10315183464639552	No Hit
TATTCAGACAGGATACCACGTGTCC	424	0.10290912444722754	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	423	0.10266641424805953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8542039833597886E-4	0.0	0.0	0.0	0.0
2	4.8542039833597886E-4	0.0	0.0	0.0	0.0
3	4.8542039833597886E-4	0.0	0.0	0.0	0.0
4	4.8542039833597886E-4	0.0	0.0	0.0	0.0
5	4.8542039833597886E-4	0.0	0.0	0.0	0.0
6	4.8542039833597886E-4	0.0	0.0	0.0	0.0
7	4.8542039833597886E-4	0.0	0.0	0.0	0.0
8	7.281305975039683E-4	0.0	0.0	0.0	0.0
9	7.281305975039683E-4	0.0	0.0	0.0	0.0
10	7.281305975039683E-4	0.0	0.0	0.0	0.0
11	7.281305975039683E-4	0.0	0.0	0.0	0.0
12	7.281305975039683E-4	0.0	0.0	2.4271019916798943E-4	0.0
13	7.281305975039683E-4	0.0	0.0	4.8542039833597886E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTTT	25	0.006022051	19.001455	8
TTAACAC	35	9.911256E-5	19.001455	3
CAGTCCC	25	0.006022051	19.001455	4
CCAGATG	50	4.5425822E-6	17.10131	3
CTTAACA	40	2.7533303E-4	16.626274	2
TCTTAGA	40	2.7533303E-4	16.626274	2
TACAGTT	35	0.002165451	16.286963	7
GTGTAAG	55	1.1221886E-5	15.5485325	1
AAAAGTA	55	1.123481E-5	15.546645	2
CACAATC	50	8.701511E-5	15.197476	17
TAACACC	45	6.743495E-4	14.77891	4
CTAGAGT	40	0.0052677123	14.251092	3
CGCAAAA	40	0.0052677123	14.251092	2
TCGACGG	40	0.0052677123	14.251092	11
AACACCT	40	0.0052677123	14.251092	5
ACAATCT	40	0.0052762055	14.247633	18
TTTTGGA	55	1.9533833E-4	13.819241	2
ACAGGGT	190	0.0	13.000997	3
ACTATCC	60	4.078053E-4	12.667638	10
AATCGCC	60	4.086416E-4	12.664563	18
>>END_MODULE