FastQCFastQC Report
Wed 25 May 2016
SRR1295240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643104
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG66040.1422324376107018No Hit
GTATCAACGCAGAGTACTTTTTTTT54610.11761528494731112No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN49400.10639434309461947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15900.012.6144981
TGGTATC16350.011.8589262
GGTATCA35000.010.7821921
TACCCCG856.5677095E-410.0638915
TAGGACG1002.7432712E-49.5048894
TACGCTG800.0044995659.5047865
TTCGCTA800.004523719.49862210
CCTAGAC3750.08.8710383
GCGTCAG2304.0017767E-108.6791291
TACATCG1107.113324E-48.6407155
CGCCTAG1000.0029104688.54875910
GCTATAC3300.08.3526013
TGCACCG2053.8342478E-88.3456665
CCGTCCA1401.0415779E-48.1417649
GTATCAA61200.08.092281
GTCCTAG5550.08.0498821
TGTACTG6850.08.0478495
GTGTACG1304.1616216E-48.0432951
CTAGACA4750.08.0041174
TCTACAC5350.07.9945933
Produced by FastQC (version 0.11.5)