FastQCFastQC Report
Wed 25 May 2016
SRR1295240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643104
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT157840.3399450023088003No Hit
GGTATCAACGCAGAGTACTTTTTTT131960.2842064274244126No Hit
TATCAACGCAGAGTACTTTTTTTTT90270.19441735528646353No Hit
GTGGTATCAACGCAGAGTACATGGG71520.15403488700662316No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57700.1242703157198288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4450.013.8770093
CAGCGTC4950.012.4751582
GTGGTAT19250.012.4391851
GGTATCA49150.012.1411061
TGGTATC19100.011.73860552
ATACCGT951.3625022E-510.99941356
GCAGCGT5350.010.8342321
TAGTACG1502.1354936E-910.7670214
GACCGTC803.7721955E-410.6879667
TAGGACG1054.1075044E-59.9527074
ACCGTCT1554.0668965E-89.8069858
GCGTCAG6500.09.6464724
CTACACT5900.09.3392914
AGACCGT1451.7096118E-69.1719266
CGTCAGA7150.08.6368345
ACCGTTC1651.0504591E-68.6368338
GTAGGAC2751.8189894E-128.636743
CCTACAC4500.08.4448143
TAGGACA5300.08.4248064
GTATAGG5200.08.4057521
Produced by FastQC (version 0.11.5)