FastQCFastQC Report
Wed 25 May 2016
SRR1295239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936598
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN46040.11695377582369346No Hit
GTGGTATCAACGCAGAGTACATGGG42440.10780882376102412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA855.2974116E-511.1826712
TATCCCG1054.0841565E-59.9577755
GGTATCA25600.09.8040621
AAGACCG1054.4710503E-49.0525235
TGTACGA950.00181556249.00499252
TTAGACG850.00740365648.9463654
CCAGGAC8400.08.9397093
GTGGTAT12850.08.5081831
GTATTAC3800.08.5062351
ATCCCGT1356.813663E-58.4433116
GTATTAG5500.08.2969911
CTAGACC3350.08.2286533
GTATCAA44150.08.2042021
TTAGACC2105.758011E-88.1475834
TGCACCG1401.0332862E-48.1472715
GTTCTAG4450.08.1183111
GAGTAGG4000.08.0809231
GTCTAAG3300.08.066521
TCTATAC4250.08.0517293
GCACCGT1304.194222E-48.0373826
Produced by FastQC (version 0.11.5)