FastQCFastQC Report
Wed 25 May 2016
SRR1295239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936598
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106390.27025873609649753No Hit
GGTATCAACGCAGAGTACTTTTTTT86460.21963126537177532No Hit
TATCAACGCAGAGTACTTTTTTTTT63530.16138300126149532No Hit
GTGGTATCAACGCAGAGTACATGGG49230.12505721945700324No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39460.10023883566470339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3450.012.1166543
GGTATCA34300.011.7456241
AAGACCG658.024253E-411.6929965
GCAGCGT3650.010.6732171
CAGCGTC3950.010.5829012
GCGTCAG4100.010.1955924
GGACCGA856.598015E-410.0587766
GTGGTAT14200.09.5017661
TACGCTG900.00111371299.5005595
CGTGAAT800.00451679859.50031812
CGTCAGA4550.09.1873545
TGGTATC13600.09.1512732
GGAACCG1054.4926995E-49.0481515
TAATACG850.0074361718.9415884
ATAGTAC4050.08.9141043
CTAGGAC3800.08.7505153
CAGTCCG1107.146086E-48.6366529
CACACCG2106.381015E-98.5957445
GTACTAA3100.08.582241
GTCTTAG4650.08.582241
Produced by FastQC (version 0.11.5)