FastQCFastQC Report
Wed 25 May 2016
SRR1295238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247921
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA272802.186035814767121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA185131.4835073694568808No Hit
GCTTATGTACTCTGCGTTGATACCA163041.3064929590895578No Hit
CTTATGTACTCTGCGTTGATACCAC107850.8642373996430863No Hit
GTATCAACGCAGAGTACTTTTTTTT83010.6651863379172239No Hit
GAGTACATAAGCAGTGGTATCAACG76270.6111765087693852No Hit
CCCATGTACTCTGCGTTGATACCAC76200.6106155758257134No Hit
TATGTACTCTGCGTTGATACCACTG65030.5211067046712091No Hit
GGTATCAACGCAGAGTACTTTTTTT56700.4543556843742512No Hit
GCGTTGATACCACTGCTTATGTACT55900.4479450221608579No Hit
GTGGTATCAACGCAGAGTACATGGG55550.44514035744249836No Hit
CATGTACTCTGCGTTGATACCACTG51800.41509037831721723No Hit
TATCAACGCAGAGTACTTTTTTTTT49540.3969802575643811No Hit
ACTCTGCGTTGATACCACTGCTTAT49230.39449612595669115No Hit
CATAAGCAGTGGTATCAACGCAGAG48880.39169146123833154No Hit
CTGCTTATGTACTCTGCGTTGATAC42550.34096709647485696No Hit
ACGCAGAGTACATAAGCAGTGGTAT41860.3354379003158053No Hit
GTATCAACGCAGAGTACATAAGCAG38060.304987254802187No Hit
TATCAACGCAGAGTACATAAGCAGT33290.26676368135482936No Hit
GGTATCAACGCAGAGTACATAAGCA31830.25506422281538654No Hit
GTACATGGGATAAGCAGTGGTATCA30850.2472111616039797No Hit
GTACATGGGAAGCAGTGGTATCAAC29570.23695410206255046No Hit
AAGCAGTGGTATCAACGCAGAGTAC29170.23374877095585378No Hit
GCTTATCCCATGTACTCTGCGTTGA27720.2221294456940784No Hit
GCAGTGGTATCAACGCAGAGTACAT23230.1861496040214084No Hit
GTGGTATCAACGCAGAGTACATAAG22910.18358533913605107No Hit
GTACTCTGCGTTGATACCACTGCTT22480.18013960819635216No Hit
ACATAAGCAGTGGTATCAACGCAGA21840.1750110784256375No Hit
TCCATGTACTCTGCGTTGATACCAC21720.17404947909362853No Hit
GGATAAGCAGTGGTATCAACGCAGA20590.16499441871721046No Hit
GTACTGGTTCACTATCGGTCAGTCA20310.16275068694252282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20290.16259042038718796No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19740.15818309011548007No Hit
GTACATGGATAAGCAGTGGTATCAA19060.15273402723409574No Hit
CCACTGCTTATGTACTCTGCGTTGA17960.14391936669067995No Hit
GTACATGATAAGCAGTGGTATCAAC17440.13975243625197428No Hit
GATACCACTGCTTATGTACTCTGCG17270.1383901705316282No Hit
GGGATAAGCAGTGGTATCAACGCAG16900.1354252392579338No Hit
ATCATGTACTCTGCGTTGATACCAC16580.13286097437257646No Hit
GGTATCAACGCAGAGTACATGGGGG16410.1314987086522304No Hit
GAGTACATGGGATAAGCAGTGGTAT16250.1302165762095517No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA15480.12404631382916068No Hit
GATAAGCAGTGGTATCAACGCAGAG15410.12348538088548876No Hit
GGTATCAACGCAGAGTACATGGGAT15320.122764181386482No Hit
ATACAGGGTGACAGCCCCGTACACA15150.12140191566613592No Hit
GTTGATACCACTGCTTATGTACTCT14990.12011978322345725No Hit
GTTAATGATAGTGTGTCGAAACACA14620.11715485194976284No Hit
ATACCACTGCTTATGTACTCTGCGT14040.11250712184505268No Hit
GGATACCACGTGTCCCGCCCTACTC13910.11146538923537629No Hit
GTATCAACGCAGAGTACATGGGATA13610.10906139090535377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN13540.10850045796168187No Hit
GAGTACATGGGAAGCAGTGGTATCA13330.10681765913066613No Hit
GGTATCAACGCAGAGTACATGGGAA12680.10160899608228405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTT307.729021E-418.99726512
ATAACGT307.729021E-418.99726513
ACGTCGC250.006039237618.99573516
TCTAGGC453.5252655E-516.8959733
TCGTTAC350.002171958816.2833714
ACTTGCT350.002172542516.28271515
CGTTACT350.002172542516.28271515
TTCGTTA350.002173126216.28205919
TACAGCT601.4684374E-615.8310547
ATAATAG704.4128865E-714.9348343
TTGAATC456.737463E-414.7845714
TGCTAGG456.7395193E-414.7839762
GTTACTA456.7724974E-414.77446116
GCACCGT653.3758515E-614.613286
GTAACAA602.5495561E-514.2605681
TACAGTA400.005263652714.2559772
TAACGTC400.005283361814.24794814
CCTGCAA400.005283361814.24794810
ACGCAAG400.005286182314.24680119
AAGATTC400.00528900414.24565618
Produced by FastQC (version 0.11.5)