FastQCFastQC Report
Wed 25 May 2016
SRR1295238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247921
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA290272.3260286508520975No Hit
GTATCAACGCAGAGTACTTTTTTTT252582.0240063273236046No Hit
GCTTATGTACTCTGCGTTGATACCA180271.4445625965105162No Hit
GGTATCAACGCAGAGTACTTTTTTT178361.4292571404760397No Hit
TATCAACGCAGAGTACTTTTTTTTT153311.2285232799191617No Hit
CTTATGTACTCTGCGTTGATACCAC126271.0118428971064675No Hit
CCCATGTACTCTGCGTTGATACCAC91020.7293730933288245No Hit
GTACTTTTTTTTTTTTTTTTTTTTT84420.6764851300683297No Hit
GAGTACATAAGCAGTGGTATCAACG83660.670395000965606No Hit
ACGCAGAGTACTTTTTTTTTTTTTT81460.6527656798787744No Hit
GCGTTGATACCACTGCTTATGTACT62950.5044389829163864No Hit
TATGTACTCTGCGTTGATACCACTG61950.4964256551496449No Hit
GTGGTATCAACGCAGAGTACATGGG61370.4917779250449348No Hit
CATGTACTCTGCGTTGATACCACTG59070.47334727118142894No Hit
CATAAGCAGTGGTATCAACGCAGAG58690.4703022066300671No Hit
ACTCTGCGTTGATACCACTGCTTAT54930.4401720942271185No Hit
CTGCTTATGTACTCTGCGTTGATAC48790.39097026173932486No Hit
ACGCAGAGTACATAAGCAGTGGTAT48490.38856626340930234No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43560.349060557519266No Hit
GTATCAACGCAGAGTACATAAGCAG38610.30939458507389495No Hit
TATCAACGCAGAGTACATAAGCAGT36090.289200999101706No Hit
AAGCAGTGGTATCAACGCAGAGTAC33940.27197234440321144No Hit
GGTATCAACGCAGAGTACATAAGCA33490.2683663469081777No Hit
GTACATGGGATAAGCAGTGGTATCA32590.2611543519181102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31870.2553847559260562No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31770.25458342314938204No Hit
GCTTATCCCATGTACTCTGCGTTGA30600.24520782966229432No Hit
GTACATGGGAAGCAGTGGTATCAAC30260.24248329822160217No Hit
GTGGTATCAACGCAGAGTACATAAG25790.20666372310426703No Hit
ACATAAGCAGTGGTATCAACGCAGA25760.20642332327126478No Hit
GTGGTATCAACGCAGAGTACTTTTT25580.20498092427325126No Hit
GCAGTGGTATCAACGCAGAGTACAT24200.1939225319551478No Hit
GGATAAGCAGTGGTATCAACGCAGA24020.19248013295713431No Hit
ATCAACGCAGAGTACTTTTTTTTTT23960.1919993332911298No Hit
TCCATGTACTCTGCGTTGATACCAC23560.18879400218443315No Hit
GTACTCTGCGTTGATACCACTGCTT22890.18342507258071625No Hit
GTACTGGTTCACTATCGGTCAGTCA21890.1754117448139746No Hit
GGGATAAGCAGTGGTATCAACGCAG20320.16283082022019021No Hit
CCACTGCTTATGTACTCTGCGTTGA20320.16283082022019021No Hit
GTACATGGATAAGCAGTGGTATCAA19840.15898442289215423No Hit
GATACCACTGCTTATGTACTCTGCG19710.15794269028247782No Hit
ATCATGTACTCTGCGTTGATACCAC18340.14696443124204175No Hit
GATAAGCAGTGGTATCAACGCAGAG17580.14087430213931812No Hit
GGTATCAACGCAGAGTACATGGGGG17310.13871070364229787No Hit
GTACATGATAAGCAGTGGTATCAAC17290.13855043708696305No Hit
GAGTACATGGGATAAGCAGTGGTAT17180.13766897103262143No Hit
ATACAGGGTGACAGCCCCGTACACA17070.13678750497827988No Hit
GGATACCACGTGTCCCGCCCTACTC16610.13310137420557872No Hit
GTTAATGATAGTGTGTCGAAACACA16500.13221990815123713No Hit
ATACCACTGCTTATGTACTCTGCGT15770.12637017888151575No Hit
GGTATCAACGCAGAGTACATGGGAT15370.12316484777481908No Hit
GTTGATACCACTGCTTATGTACTCT15060.12068071616712917No Hit
GAGTACATGGGAAGCAGTGGTATCA14950.1197992501127876No Hit
GTATCAACGCAGAGTACATGGGATA14520.11635351917308868No Hit
CCCCATGTACTCTGCGTTGATACCA14310.11467072034207293No Hit
CTTATACACATCTCCGAGCCCACGA14140.11330845462172685No Hit
CAGTGGTATCAACGCAGAGTACATA13320.10673752585299871No Hit
GGTATCAACGCAGAGTACATGGGAA13210.10585605979865713No Hit
GCGTTGATACCACTGCTTATCCCAT13090.10489446046664813No Hit
ACGCAGAGTACATGGGATAAGCAGT13040.10449379407831104No Hit
TACCACTGCTTATGTACTCTGCGTT12760.10225006230362339No Hit
CAGTGGTATCAACGCAGAGTACATG12750.10216992902595597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG307.716963E-419.0016825
GACTAGG250.0060325318.99942
CTAGTTC453.5389457E-516.8883533
TCATCGG350.00217033616.28523
TATACAC1800.015.8328323
GACATAG456.7585224E-414.7784947
TTTAGAC456.762631E-414.7773093
ATAGCAC456.762631E-414.7773093
CGAGACA902.2919266E-914.77494118
AACTGTG653.3691394E-614.6160947
GGAATCC851.7125785E-814.5312818
AGCCCCT400.005275230414.2512625
GTAGGTC400.005285057214.24726516
GACCCGA759.667383E-713.9334526
CCAGGAC759.671749E-713.9328933
ATTCACC551.9575263E-413.81996111
GCTAGAC707.2621733E-613.5709993
CCTAGTT500.001499616413.299582
GACCGAT500.001501428913.29744718
GCGTTCA500.001501428913.29744719
Produced by FastQC (version 0.11.5)