FastQCFastQC Report
Wed 25 May 2016
SRR1295237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643471
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG68440.12127288330178361No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN64450.11420276634716471No Hit
GGTATCAACGCAGAGTACATGGGGG64370.11406100961624505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.010.6927351
GTGGTAT18050.010.1643531
TGGTATC18100.09.5048562
CAACCGT1106.8543E-59.4985996
GTATCAA71900.09.1887311
GTATTAC5550.08.906561
GTCCTAA4850.08.8200371
GTCCTGG13950.08.7223881
ATCGACC2106.4064807E-98.5941238
AGGACCG3100.08.5849545
GTATTAG6550.08.5626921
GTCCTAG6250.08.5174121
TGGACTG11550.08.4761165
GGACTGT9200.08.4661436
GTACGGG2252.4283509E-98.4491374
GTCTTAG4300.08.4006861
TACCGAT1150.00111290438.2597257
GTATAGA7800.08.1654451
TTAGACG1401.0333673E-48.1473824
ATTAGAC3150.08.1473093
Produced by FastQC (version 0.11.5)