##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5643471 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.204955071090115 31.0 30.0 33.0 26.0 34.0 2 30.452114487697376 31.0 30.0 34.0 26.0 34.0 3 30.396117566653572 31.0 30.0 34.0 26.0 34.0 4 33.57881824855661 35.0 35.0 37.0 28.0 37.0 5 34.060965848854366 35.0 35.0 37.0 29.0 37.0 6 34.196844282534634 37.0 35.0 37.0 30.0 37.0 7 34.12578021575729 37.0 35.0 37.0 29.0 37.0 8 34.118030729669734 37.0 35.0 37.0 29.0 37.0 9 35.14984412961456 39.0 35.0 39.0 27.0 39.0 10 35.155238150421965 39.0 35.0 39.0 27.0 39.0 11 35.61717868311895 39.0 35.0 39.0 29.0 39.0 12 35.60283520549676 39.0 35.0 39.0 29.0 39.0 13 35.55138991588687 39.0 35.0 39.0 29.0 39.0 14 36.21265086681583 39.0 36.0 41.0 27.0 41.0 15 36.567382378681486 40.0 36.0 41.0 28.0 41.0 16 36.529335580886304 40.0 36.0 41.0 27.0 41.0 17 36.174564731527816 39.0 36.0 41.0 27.0 41.0 18 36.06125698174049 39.0 36.0 41.0 27.0 41.0 19 36.48220766971249 40.0 36.0 41.0 27.0 41.0 20 36.50924050110296 40.0 36.0 41.0 27.0 41.0 21 36.55764138772043 40.0 36.0 41.0 27.0 41.0 22 36.465673696205755 40.0 36.0 41.0 27.0 41.0 23 36.15537671762644 39.0 36.0 41.0 27.0 41.0 24 36.43554294865695 39.0 36.0 41.0 27.0 41.0 25 35.10200087853734 39.0 34.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14273853025581218 1101 2 0.06706843799640083 1101 3 -0.11379965648227852 1101 4 -0.05027319229657934 1101 5 -0.10645230322774779 1101 6 -0.2608246430342689 1101 7 -0.14388429184261753 1101 8 -0.11573518053759813 1101 9 0.013742677153196325 1101 10 0.10316501221289798 1101 11 0.10204568444314788 1101 12 0.025156028745385584 1101 13 0.0736855896265709 1101 14 0.4817108666127865 1101 15 0.30453244572152016 1101 16 0.14923377654453418 1101 17 0.20963221641842011 1101 18 0.37624300022129376 1101 19 0.1533054403999472 1101 20 0.20476826768540235 1101 21 0.14817876409345132 1101 22 0.06766944494869165 1101 23 0.16182023194372164 1101 24 -0.0023199299101577253 1101 25 0.0064742668529262914 1102 1 -0.25504918539044397 1102 2 0.046183559340676084 1102 3 -0.1325224805452052 1102 4 -0.24001653558488556 1102 5 -0.3639167930754539 1102 6 -0.44466835088453394 1102 7 -0.4183299292142948 1102 8 -0.2979310354882543 1102 9 -0.23479417884935572 1102 10 0.135137660135058 1102 11 -0.011250993319926295 1102 12 -0.10448633500573123 1102 13 -0.1895953753455828 1102 14 0.04452548085630781 1102 15 -0.08313052473059201 1102 16 -0.17332191352588922 1102 17 -0.32157309790150634 1102 18 0.10415250426638067 1102 19 -0.02031954219833665 1102 20 0.046057450822530654 1102 21 -0.01796766135704786 1102 22 -0.16359945527098319 1102 23 -0.13938652607013324 1102 24 -0.11933207829991233 1102 25 -0.24844984178076857 1103 1 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12 -0.5225067957306919 1104 13 -0.3784562808180638 1104 14 -0.17253709540653972 1104 15 -0.288229986432583 1104 16 -0.34273628527439826 1104 17 4.059377232579209E-5 1104 18 0.10608528928218419 1104 19 -0.2171643805813801 1104 20 -0.3087013112753496 1104 21 -0.2306214911855946 1104 22 -0.2712528184803773 1104 23 -0.21569731895471023 1104 24 -0.23996416978580726 1104 25 -0.2923788748148368 1105 1 -0.22020805263801435 1105 2 -0.15271962660724014 1105 3 -0.20875073449445125 1105 4 -0.11526441208957294 1105 5 -0.17250600482611844 1105 6 -0.24404323131404482 1105 7 -0.23051860183471717 1105 8 -0.24167443801784572 1105 9 -2.253837398347649 1105 10 -0.6619748372703782 1105 11 -0.37672445009268785 1105 12 -0.3137266152056952 1105 13 -0.15414768132293943 1105 14 -0.10323072585654103 1105 15 -0.116956111323951 1105 16 -0.1647466359221781 1105 17 -1.5268645433495465 1105 18 -0.2493881101597495 1105 19 -0.2011264398691779 1105 20 -0.15346102748036827 1105 21 -0.14807012478872394 1105 22 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0.1076395892384312 2214 1 0.3703131718987507 2214 2 0.2760401068543601 2214 3 0.3687823272661461 2214 4 0.6923655125710368 2214 5 0.3963391417592135 2214 6 0.28385864983417264 2214 7 0.1854775623687246 2214 8 0.11301098651718888 2214 9 0.3634851548005784 2214 10 0.21939572717534617 2214 11 0.15824751349787647 2214 12 0.02214263144752948 2214 13 -0.07461731301387431 2214 14 0.1496345280061533 2214 15 0.05565022804294273 2214 16 0.06951700945350581 2214 17 0.13037847293747262 2214 18 0.39205630574800665 2214 19 0.18434030786125533 2214 20 0.285464622433409 2214 21 0.16269695807650209 2214 22 0.22331269422293332 2214 23 0.3145694140314603 2214 24 0.27547228201721197 2214 25 0.27822592045155403 2215 1 0.40293470275906174 2215 2 0.24099801135219678 2215 3 0.37096833207363744 2215 4 0.6965239570648336 2215 5 0.40874487757191247 2215 6 0.47935818992157664 2215 7 0.4287884574240479 2215 8 0.4147216912481184 2215 9 0.6359179085657161 2215 10 0.6155694789547042 2215 11 0.3201179191375232 2215 12 0.20187416936590097 2215 13 0.1825406263100362 2215 14 0.351687115885305 2215 15 0.21274965967884185 2215 16 0.34679128153346994 2215 17 0.5982564191415207 2215 18 0.7116044377943069 2215 19 0.3587497832486264 2215 20 -0.5119498818815416 2215 21 0.005537434099139205 2215 22 -0.7159488095442157 2215 23 0.18648563560761744 2215 24 0.1324176002687949 2215 25 0.20950494638815798 2216 1 0.2648396760640779 2216 2 -0.27601377860134946 2216 3 0.3691783618337183 2216 4 0.7213442618731207 2216 5 0.49840896671005197 2216 6 0.4884917674862166 2216 7 0.38323541426817087 2216 8 0.39720056205719345 2216 9 0.82935444671417 2216 10 0.413941302886208 2216 11 0.3275408006610121 2216 12 -0.006047143034273006 2216 13 0.18547173834919306 2216 14 0.1855532217886804 2216 15 0.2147748418384836 2216 16 0.3408560840239261 2216 17 0.43898124939828165 2216 18 0.7616269718241924 2216 19 0.4280205470985834 2216 20 -0.8849891837474644 2216 21 -1.1407491426565812 2216 22 -1.066860380618884 2216 23 -1.5118241257636171 2216 24 -1.2226186766513436 2216 25 -0.903407267992371 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11583.0 3 1549.0 4 1275.0 5 985.0 6 1179.0 7 1550.0 8 737.0 9 1401.0 10 3052.0 11 6056.0 12 10724.0 13 16230.0 14 21697.0 15 26703.0 16 30677.0 17 32884.0 18 33889.0 19 34675.0 20 34902.0 21 35806.0 22 36860.0 23 39042.0 24 41731.0 25 45306.0 26 49961.0 27 57233.0 28 66138.0 29 79846.0 30 99062.0 31 125717.0 32 164225.0 33 219736.0 34 301661.0 35 425154.0 36 626838.0 37 1013595.0 38 1943812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51603244054923 19.484594678573057 13.685020459450822 24.31435242142689 2 15.79636606098647 19.991125117888966 40.034497238994675 24.17801158212989 3 18.391111789180606 25.44583217531036 29.403736862329428 26.759319173179602 4 14.025687457234854 17.199241517271382 36.54915970877991 32.22591131671385 5 13.928257598127283 37.30302502054509 35.01801920388205 13.750698177445578 6 31.52690606999033 37.58622817169516 17.295099354092162 13.59176640422234 7 27.53217444462087 31.988738877703593 22.18731991085982 18.291766766815716 8 25.56775166623303 36.110053453548836 19.847170902859375 18.475023977358756 9 25.901224891293257 17.165103738615887 20.34947258513124 36.58419878495962 10 15.9304448671873 29.109296222084076 32.77670016888962 22.183558741839 11 33.48276577264907 24.368080107730695 23.4823267881996 18.66682733142064 12 22.55527039276482 25.780510156509205 30.124617759568768 21.539601691157202 13 27.480045427615245 22.745359516486353 26.14658245007291 23.628012605825493 14 22.3012257818268 22.07034836765384 26.654670356580894 28.97375549393847 15 23.362607961380398 29.87228527058549 23.484165664208362 23.28094110382575 16 23.343876169404233 29.32538469975137 24.853345079592103 22.477394051252297 17 22.54406295439477 28.832673171536783 26.455130339271943 22.168133534796503 18 22.628513011978576 28.62542836189079 27.133968931262086 21.612089694868548 19 23.201698468497785 28.01439928590353 27.3032011650299 21.480701080568785 20 23.54609490382901 27.471107643856552 27.150291798621712 21.832505653692724 21 23.746392570161508 27.273538757532705 26.814241590665404 22.165827081640384 22 23.819021632489402 26.767255230387292 27.01910580781295 22.39461732931036 23 23.849114824003486 27.018290657738287 26.81357432757705 22.319020190681176 24 23.469279278734756 27.13396759525751 26.793430166183548 22.603322959824194 25 23.57611804350852 27.14793070648237 26.74906973027733 22.526881519731777 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14564.0 1 14564.0 2 12361.5 3 10159.0 4 10159.0 5 10159.0 6 9509.0 7 8859.0 8 8859.0 9 8859.0 10 11743.5 11 14628.0 12 14628.0 13 14628.0 14 23283.5 15 31939.0 16 31939.0 17 31939.0 18 52097.5 19 72256.0 20 72256.0 21 72256.0 22 108917.0 23 145578.0 24 145578.0 25 145578.0 26 197794.5 27 250011.0 28 250011.0 29 250011.0 30 315222.0 31 380433.0 32 380433.0 33 380433.0 34 443018.0 35 505603.0 36 505603.0 37 505603.0 38 559900.0 39 614197.0 40 614197.0 41 614197.0 42 647724.5 43 681252.0 44 681252.0 45 681252.0 46 680281.0 47 679310.0 48 679310.0 49 679310.0 50 665979.0 51 652648.0 52 652648.0 53 652648.0 54 607699.5 55 562751.0 56 562751.0 57 562751.0 58 490767.5 59 418784.0 60 418784.0 61 418784.0 62 352870.0 63 286956.0 64 286956.0 65 286956.0 66 228168.0 67 169380.0 68 169380.0 69 169380.0 70 126995.0 71 84610.0 72 84610.0 73 84610.0 74 61191.0 75 37772.0 76 37772.0 77 37772.0 78 26236.0 79 14700.0 80 14700.0 81 14700.0 82 9949.0 83 5198.0 84 5198.0 85 5198.0 86 3319.0 87 1440.0 88 1440.0 89 1440.0 90 891.5 91 343.0 92 343.0 93 343.0 94 211.0 95 79.0 96 79.0 97 79.0 98 50.0 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14491081818263973 2 0.28978619718254955 3 0.34840260541783596 4 0.3755490193889541 5 0.384355656297339 6 0.3418817957955308 7 0.3458864234440117 8 0.3625074001443438 9 0.34774698053733244 10 0.3801915523265735 11 0.29853967531683956 12 0.3436360353406618 13 0.3157276789408504 14 0.3261822378461766 15 0.365502011085022 16 0.3162947058645291 17 0.36514761925772277 18 0.3339434188640289 19 0.3469673185172742 20 0.31012828806952314 21 0.348384885826471 22 0.30334168457674365 23 0.2865612315541269 24 0.32329394445368814 25 0.318102104183755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5643471.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.85880279332845 #Duplication Level Percentage of deduplicated Percentage of total 1 71.4818320293073 37.784440625429845 2 14.296287464035359 15.113692794763574 3 5.725628599223794 9.079496189826358 4 2.86684156248985 6.061512511654743 5 1.596205106402985 4.218674546852962 6 1.003666686278211 3.1831571664128053 7 0.6600041257250767 2.4420919547139492 8 0.4697187088210987 1.9863014878329057 9 0.34203197554096654 1.6271460669719244 >10 1.4755475854755244 13.242249605586217 >50 0.05945565852919651 2.1295529928591095 >100 0.021567501627732234 1.8881865004913307 >500 6.401982035028906E-4 0.22077925118381453 >1k 4.38022259559855E-4 0.5724394241780735 >5k 1.347760798634608E-4 0.45027888124240123 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 6844 0.12127288330178361 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 6445 0.11420276634716471 No Hit GGTATCAACGCAGAGTACATGGGGG 6437 0.11406100961624505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7719591364959613E-5 0.0 5 0.0 0.0 0.0 1.7719591364959613E-5 0.0 6 0.0 0.0 0.0 1.7719591364959613E-5 0.0 7 0.0 0.0 0.0 1.7719591364959613E-5 0.0 8 0.0 1.7719591364959613E-5 0.0 1.7719591364959613E-5 0.0 9 7.087836545983845E-5 1.7719591364959613E-5 0.0 1.7719591364959613E-5 0.0 10 7.087836545983845E-5 3.5439182729919226E-5 0.0 3.5439182729919226E-5 0.0 11 1.0631754818975769E-4 3.5439182729919226E-5 0.0 3.5439182729919226E-5 0.0 12 1.0631754818975769E-4 3.5439182729919226E-5 0.0 3.5439182729919226E-5 0.0 13 1.0631754818975769E-4 5.315877409487884E-5 0.0 5.315877409487884E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3805 0.0 10.692735 1 GTGGTAT 1805 0.0 10.164353 1 TGGTATC 1810 0.0 9.504856 2 CAACCGT 110 6.8543E-5 9.498599 6 GTATCAA 7190 0.0 9.188731 1 GTATTAC 555 0.0 8.90656 1 GTCCTAA 485 0.0 8.820037 1 GTCCTGG 1395 0.0 8.722388 1 ATCGACC 210 6.4064807E-9 8.594123 8 AGGACCG 310 0.0 8.584954 5 GTATTAG 655 0.0 8.562692 1 GTCCTAG 625 0.0 8.517412 1 TGGACTG 1155 0.0 8.476116 5 GGACTGT 920 0.0 8.466143 6 GTACGGG 225 2.4283509E-9 8.449137 4 GTCTTAG 430 0.0 8.400686 1 TACCGAT 115 0.0011129043 8.259725 7 GTATAGA 780 0.0 8.165445 1 TTAGACG 140 1.0333673E-4 8.147382 4 ATTAGAC 315 0.0 8.147309 3 >>END_MODULE