FastQCFastQC Report
Wed 25 May 2016
SRR1295237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643471
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT151560.26855812672732793No Hit
GGTATCAACGCAGAGTACTTTTTTT112120.1986720583839272No Hit
TATCAACGCAGAGTACTTTTTTTTT87020.15419588405787857No Hit
GTGGTATCAACGCAGAGTACATGGG75980.13463345519096315No Hit
GGTATCAACGCAGAGTACATGGGGG70730.12533066972435936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.011.8270221
GTGGTAT19000.011.1524081
TGGTATC18600.010.3176042
AGGACCG2300.09.9135165
TCGACCG1451.6198283E-79.8276199
AGCGTCA3600.09.7643543
GACCGTG3250.09.3539597
GTCTTAG5350.09.058031
GTATCAA87900.08.7885871
CCGATCA1304.3730237E-58.7692599
TCTATAC5500.08.4640393
TATACCG2252.4501787E-98.4448465
CGGTTTA1701.6158192E-68.38163815
AACACCG1502.5872347E-58.2337255
GTAATAC4650.08.1724323
GCGTTAT1050.0045068748.1446161
TATATCG1401.03949846E-48.1432455
TAAGACT6200.08.1212824
TAGGACC4100.08.110074
GTTCTAG6150.08.0342551
Produced by FastQC (version 0.11.5)