FastQCFastQC Report
Wed 25 May 2016
SRR1295236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418972
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG68620.1266291835425612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN61140.11282582748166996No Hit
GTATCAACGCAGAGTACTTTTTTTT60990.11254902221306919No Hit
ATTCCATTCCATTCCATTCCATTCC59610.1100024137419422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT700.001485549710.8631654
AGCGCGC700.001494418410.85541610
CGGTCGT700.001494418410.85541610
GGTATCA41300.010.7259691
GGACCGA1554.0792656E-89.8051666
CCGGTCG900.00111560139.4989319
GTGGTAT19600.09.1665611
TGGTATC18750.08.7193392
GTATTAG9850.08.5892341
CTGCACG1901.0038639E-78.5047154
GTTCTAG7000.08.2838551
TAGACTG6850.08.1869445
TGGACCG1659.684136E-68.0650025
TAGGACT6300.07.9964974
GTAGGAC3450.07.98978853
AGGACGG1553.8753802E-57.9720885
CGGGACT1200.00168278117.9210584
TCTATAC6900.07.85203363
ACAGTCC6200.07.8136368
GTATCAA69850.07.7844931
Produced by FastQC (version 0.11.5)