##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295236_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5418972
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.10108061086125	31.0	30.0	33.0	26.0	34.0
2	30.376044755352122	31.0	30.0	34.0	26.0	34.0
3	30.36123272089245	31.0	30.0	34.0	26.0	34.0
4	33.76634775008987	35.0	35.0	37.0	28.0	37.0
5	33.83642469457307	35.0	35.0	37.0	28.0	37.0
6	34.13482631023006	37.0	35.0	37.0	30.0	37.0
7	34.10671119909828	37.0	35.0	37.0	29.0	37.0
8	34.24547331117415	37.0	35.0	37.0	30.0	37.0
9	35.24522603180087	39.0	35.0	39.0	27.0	39.0
10	35.28939012786927	39.0	35.0	39.0	28.0	39.0
11	35.664647464500646	39.0	35.0	39.0	29.0	39.0
12	35.666686781182854	39.0	35.0	39.0	29.0	39.0
13	35.59084306027047	39.0	35.0	39.0	29.0	39.0
14	36.24928325889117	39.0	36.0	41.0	27.0	41.0
15	36.610654566954764	40.0	36.0	41.0	28.0	41.0
16	36.58801005061476	40.0	36.0	41.0	28.0	41.0
17	36.262797999325336	39.0	36.0	41.0	27.0	41.0
18	36.17176412795637	39.0	36.0	41.0	27.0	41.0
19	36.61515099173791	40.0	36.0	41.0	27.0	41.0
20	36.630618501073634	40.0	36.0	41.0	28.0	41.0
21	36.67910832534289	40.0	36.0	41.0	28.0	41.0
22	36.57836652413041	40.0	36.0	41.0	27.0	41.0
23	36.29594081681913	39.0	36.0	41.0	27.0	41.0
24	36.58699915777384	40.0	36.0	41.0	27.0	41.0
25	35.32537462825052	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.3721353598254211
1101	2	-0.006263721262190103
1101	3	-0.2919763251481058
1101	4	-0.255848928533311
1101	5	-0.4791627058828709
1101	6	-0.4827733110046495
1101	7	-0.5094406287838353
1101	8	-0.32279195562968255
1101	9	-0.23547698402028772
1101	10	-0.1277678406369347
1101	11	-0.20081257448399725
1101	12	-0.22673809052917449
1101	13	-0.17202458837996204
1101	14	0.1590193540631475
1101	15	-0.04405383162050214
1101	16	-0.17973031909251347
1101	17	-0.1528405868426077
1101	18	0.11240482819537334
1101	19	-0.1060902081870907
1101	20	-0.09336086715079972
1101	21	-0.11419401405439089
1101	22	-0.22230390093701402
1101	23	-0.1422558182657383
1101	24	-0.30601839213778703
1101	25	-0.3578651990931192
1102	1	-0.45339735858860664
1102	2	-0.0742099326321295
1102	3	-0.35959944681091116
1102	4	-0.2871971004788918
1102	5	-0.8536097474198954
1102	6	-0.6488819768138612
1102	7	-0.5773610236731841
1102	8	-0.504270838892424
1102	9	-0.40852114670842354
1102	10	-0.06288597166852838
1102	11	-0.2569543412782096
1102	12	-0.23811704822967528
1102	13	-0.37943540966982425
1102	14	-0.2794397452037529
1102	15	-0.2301469293534737
1102	16	-0.3929190366968456
1102	17	-0.5616131659466674
1102	18	-0.18948617471046703
1102	19	-0.3405655479460208
1102	20	-0.38511410155908976
1102	21	-0.2495066591635009
1102	22	-0.39735230864801707
1102	23	-0.3486165907678114
1102	24	-0.3938317680864074
1102	25	-0.5318786453818802
1103	1	-0.6521169210632927
1103	2	-0.4123513533073542
1103	3	-0.707515694629258
1103	4	-0.5162204481769024
1103	5	-0.8775162983533065
1103	6	-0.7916732694307669
1103	7	-0.7838162411392346
1103	8	-0.5839984345136742
1103	9	-0.20567632891836496
1103	10	-0.10275756370006661
1103	11	-0.26963124095566116
1103	12	-0.4668732071210613
1103	13	-0.4470585073700235
1103	14	-0.5019190067486718
1103	15	-0.513317239636315
1103	16	-0.45800034426117264
1103	17	-0.3242578689358595
1103	18	-0.13849419620211734
1103	19	-0.41831705361467186
1103	20	-0.3450483095934018
1103	21	-0.48875238254004216
1103	22	-0.4212464762397943
1103	23	-2.6431951637989357
1103	24	-1.275653605308115
1103	25	-0.8130272658776221
1104	1	-0.5387997297799991
1104	2	-0.16380413781098824
1104	3	-0.49274571884233254
1104	4	-0.6495134263851341
1104	5	-0.6189206567068197
1104	6	-0.5491793226995298
1104	7	-0.5841794372651421
1104	8	-0.4384778533998741
1104	9	-2.6853373458675165
1104	10	-0.9240650697533397
1104	11	-0.730648190108198
1104	12	-0.5696577107530203
1104	13	-0.38834180034822907
1104	14	-0.14973982999322288
1104	15	-0.40992740912783887
1104	16	-0.42155966629507446
1104	17	-0.05307142825789413
1104	18	0.09697795876156334
1104	19	-0.20209429332411588
1104	20	-0.3468642902229391
1104	21	-0.38645214040928977
1104	22	-0.37209393386691403
1104	23	-0.3056648974551095
1104	24	-0.3078569951386214
1104	25	-0.4468045055870675
1105	1	-0.35390114157144836
1105	2	-0.32859899284663285
1105	3	-0.34383530722005773
1105	4	-0.08691920883093474
1105	5	-0.3460286361524396
1105	6	-0.32007418091995277
1105	7	-0.37308773349386826
1105	8	-0.35201223774876667
1105	9	-2.1432756815611924
1105	10	-0.7010706103897846
1105	11	-0.4345417852283049
1105	12	-0.39958946301472764
1105	13	-0.310556529740019
1105	14	-0.34469522995891566
1105	15	-0.2971499353810714
1105	16	-0.38370737218875206
1105	17	-1.5944483069479602
1105	18	-0.30320294537049364
1105	19	-0.4188405112546505
1105	20	-0.31651340370065384
1105	21	-0.2752600138807111
1105	22	-0.2395181441954648
1105	23	-0.25599932831928385
1105	24	-0.23138338540360337
1105	25	-0.901154897154008
1106	1	-0.31441930865639023
1106	2	-0.37299407252763217
1106	3	-0.3986454338023364
1106	4	-0.3349451240588408
1106	5	-0.40209563448595986
1106	6	-0.4308996770073108
1106	7	-0.4453894780289289
1106	8	-0.3933986245403389
1106	9	-3.049497703853234
1106	10	-0.7224280819824713
1106	11	-0.614376084183661
1106	12	-0.42354896646148177
1106	13	-0.46050473288876503
1106	14	-0.7062454848189788
1106	15	-0.5785129364518298
1106	16	-0.5652844954243221
1106	17	-0.44523987770161
1106	18	-0.14741165720067784
1106	19	-0.3552573178130345
1106	20	-0.3776538203666888
1106	21	-0.34560682824452016
1106	22	-0.3642130376766417
1106	23	-0.18372999691261072
1106	24	-0.349902233744686
1106	25	-0.6159999572068884
1107	1	-0.41584830194855016
1107	2	-0.28296378905006847
1107	3	-0.29449435306165483
1107	4	-1.5883035041364124
1107	5	-0.5999386565141975
1107	6	-0.4142897727893313
1107	7	-0.3181841070304898
1107	8	-0.25788419670021057
1107	9	-0.05196924018972027
1107	10	0.23662003345796734
1107	11	-0.12301279614511884
1107	12	-0.7114582252914587
1107	13	-0.36892318528641255
1107	14	-0.10834095086117657
1107	15	-0.8057925174737903
1107	16	-1.2456652610603243
1107	17	-0.3257281784751598
1107	18	-0.4463109834634338
1107	19	-0.2558161815993998
1107	20	-0.5878228969642478
1107	21	-0.29004876916295075
1107	22	-0.3225145347228562
1107	23	-0.05246215129190546
1107	24	-0.26950394178597037
1107	25	-0.48879305062499157
1108	1	0.261607879217749
1108	2	0.32900253334699414
1108	3	0.35083870563577335
1108	4	0.6948887730247719
1108	5	0.5488011352830782
1108	6	0.4644911704778991
1108	7	0.344073041295502
1108	8	0.3067876455250982
1108	9	0.6392697753112486
1108	10	0.6979322532455257
1108	11	0.437590881212536
1108	12	0.3483554052184914
1108	13	0.30675799798731873
1108	14	0.39359707193381865
1108	15	0.4017554551744382
1108	16	0.4206938728382852
1108	17	0.4869611893556183
1108	18	0.6340310785573635
1108	19	0.4257646393884329
1108	20	0.4246855689232234
1108	21	0.393574629602071
1108	22	0.46290724454721044
1108	23	0.6704239127246083
1108	24	0.4719190283585135
1108	25	0.5355860408635778
1109	1	0.3082435441200744
1109	2	0.207712708450412
1109	3	0.3564937521240914
1109	4	0.687718647958043
1109	5	0.6245213309577835
1109	6	0.4371441362892128
1109	7	0.3847018571246963
1109	8	0.3355467481613772
1109	9	0.7586782358905566
1109	10	0.47083624129140134
1109	11	0.36938113867612543
1109	12	0.3875462079572003
1109	13	0.34132277451902127
1109	14	0.8610218144957571
1109	15	0.5563873762335376
1109	16	0.5109899955103856
1109	17	0.7930196698310255
1109	18	0.6174026203266223
1109	19	0.3579988472021114
1109	20	0.44682155907133847
1109	21	0.4002660551685011
1109	22	0.49203157794441665
1109	23	0.6434840869996705
1109	24	0.3786559951088364
1109	25	0.3543749923426134
1110	1	0.42086022898622844
1110	2	0.36947829937198406
1110	3	0.48034175153738445
1110	4	0.686848771947723
1110	5	0.6416551743408121
1110	6	0.658803769767978
1110	7	0.5463850203562615
1110	8	0.4968706383358281
1110	9	1.0766480854834768
1110	10	0.9938684513326592
1110	11	0.6069021109842865
1110	12	0.5563078267590171
1110	13	0.5464919493215987
1110	14	0.8622120060901608
1110	15	0.6886558498742659
1110	16	0.6732034571453696
1110	17	1.0744460201992112
1110	18	1.1276163939783288
1110	19	0.667434486123085
1110	20	0.6614906080078811
1110	21	0.662613951955116
1110	22	0.7363342482917972
1110	23	0.8585566185864266
1110	24	0.6699619402423096
1110	25	0.7079168264285727
1111	1	0.22508231406428791
1111	2	0.11045150198163256
1111	3	0.2548242406613461
1111	4	0.5362251329162717
1111	5	0.5254059939864959
1111	6	0.3779108119370491
1111	7	0.251537777782886
1111	8	0.2130530741757184
1111	9	0.6070221678245744
1111	10	0.46307404114298834
1111	11	0.233327647426826
1111	12	0.1464410685812254
1111	13	0.21174129371033956
1111	14	0.1588826432501378
1111	15	0.18613496120101303
1111	16	0.09044299678561174
1111	17	0.4246744783076366
1111	18	0.4605137911145576
1111	19	0.2594801781786913
1111	20	-0.2155994286676517
1111	21	-0.34179190324053366
1111	22	-0.16187951213802165
1111	23	0.17129019902174036
1111	24	0.13948244092750173
1111	25	0.1071416645359875
1112	1	0.30850054607369515
1112	2	0.18708702475384698
1112	3	0.40847667049581915
1112	4	0.8133652317114084
1112	5	0.7166995419887741
1112	6	0.5613537696271536
1112	7	0.5397929908544015
1112	8	0.4586499633091705
1112	9	-0.9910198645668942
1112	10	0.29699295394714653
1112	11	0.3012806274978743
1112	12	0.27524162625386595
1112	13	0.318529856864771
1112	14	0.6374768804008681
1112	15	0.3855771873096714
1112	16	0.3718277361613076
1112	17	0.41882380129419516
1112	18	0.5444105634544485
1112	19	0.4455987874018703
1112	20	0.3821451994139977
1112	21	0.4248060626972645
1112	22	0.43230401038231747
1112	23	0.21715643163363296
1112	24	0.33834465458788543
1112	25	0.42690967221379594
1113	1	0.41473766189298544
1113	2	0.23685377301641708
1113	3	0.39959903621428694
1113	4	0.5645194229584476
1113	5	0.7039230616171253
1113	6	0.5125627958222481
1113	7	0.43979286580320576
1113	8	0.3993878903980743
1113	9	0.29653178939144453
1113	10	0.5418359365431513
1113	11	0.39248834065544713
1113	12	0.20591330747335945
1113	13	0.2682415200768986
1113	14	0.048971523520393134
1113	15	0.2124839522665738
1113	16	0.2586925464430152
1113	17	0.6671570877333366
1113	18	0.4310160800878222
1113	19	0.3460482839145058
1113	20	0.38151650454203434
1113	21	0.36834251043055843
1113	22	0.403813784696176
1113	23	0.10510149664462176
1113	24	0.27117165564143875
1113	25	0.21979938644055608
1114	1	0.5398412811723183
1114	2	0.4397394910057848
1114	3	0.544395470448034
1114	4	0.7619932286915514
1114	5	0.7668460282584206
1114	6	0.6267570582889732
1114	7	0.3846359439467122
1114	8	0.36560916866278603
1114	9	0.2423748863697881
1114	10	0.45129600835925743
1114	11	0.5359093889136162
1114	12	0.5300531372729509
1114	13	0.5811445993504947
1114	14	0.9197589192502065
1114	15	0.6552458921657305
1114	16	0.46885848132102836
1114	17	0.9730239166011501
1114	18	0.7973530263852808
1114	19	0.5620022737414772
1114	20	0.4543323009840279
1114	21	0.6197264828740856
1114	22	0.35537823915631606
1114	23	0.8217195805216662
1114	24	0.7135821597169709
1114	25	0.6921184972591732
1115	1	0.5600727151450258
1115	2	0.4084591426233217
1115	3	0.6060933191113342
1115	4	0.7844095384417002
1115	5	0.8182252898829674
1115	6	0.5838741303157491
1115	7	0.5985105404573403
1115	8	0.5642463501835095
1115	9	0.8236649488843284
1115	10	0.5162431863283103
1115	11	0.5493041840201016
1115	12	0.5604667232361038
1115	13	0.39553928944486927
1115	14	0.516624654022614
1115	15	0.4942331081125815
1115	16	0.5972765613785569
1115	17	0.8125163204100119
1115	18	0.9551115792025371
1115	19	0.6851800700357842
1115	20	0.6469075894821827
1115	21	0.49793647479264536
1115	22	-0.018701683912041744
1115	23	0.5185527373313192
1115	24	0.5220706261919617
1115	25	0.645009948930138
1116	1	0.7724559778285709
1116	2	0.5399561055337898
1116	3	0.7838527150640644
1116	4	1.032083894664055
1116	5	1.1054460600946285
1116	6	0.8547242431647533
1116	7	0.8185673641134485
1116	8	0.6641095733370292
1116	9	1.0007350100849166
1116	10	0.75392888709478
1116	11	0.5545396804226641
1116	12	0.5924561109335258
1116	13	0.577025678591788
1116	14	1.0711259054705025
1116	15	0.7407861009586938
1116	16	0.8490422208409711
1116	17	1.1977653054724726
1116	18	1.1777253266856462
1116	19	0.8188578403266646
1116	20	0.7383568985783455
1116	21	0.7996250042014452
1116	22	0.8901313084324514
1116	23	1.1242316405472792
1116	24	0.8489853231115632
1116	25	0.8067425412945752
1201	1	-0.5421304048342357
1201	2	-0.07133318869167482
1201	3	-0.4613265796409074
1201	4	-0.31363311481850076
1201	5	-0.7398608713686841
1201	6	-0.7005958764913487
1201	7	-0.6903331201913261
1201	8	-0.5580583670481829
1201	9	-0.1684236516203299
1201	10	-0.10649983479731873
1201	11	-0.3153126741878225
1201	12	-0.2682978057048899
1201	13	-0.2968994769889832
1201	14	0.06941053503921779
1201	15	-0.2406030754683428
1201	16	-0.2834251515432271
1201	17	-0.23091937935721774
1201	18	-0.07826050665355666
1201	19	-0.32577966209184694
1201	20	-0.2016884838272759
1201	21	-0.39056241701896965
1201	22	-0.42569972040418236
1201	23	-0.5097088191046666
1201	24	-0.5996161550524732
1201	25	-0.5013835516437908
1202	1	-0.6702236670291697
1202	2	-0.4255988269769908
1202	3	-0.7587790866904953
1202	4	-0.6415498540212354
1202	5	-1.0453816161605332
1202	6	-0.8704556365977041
1202	7	-0.7778167938448988
1202	8	-0.6374553332786661
1202	9	-0.16703056611867595
1202	10	-0.053081195116817526
1202	11	-0.36494306443645996
1202	12	-0.3810562551581498
1202	13	-0.3078433523445696
1202	14	-0.48900441528978433
1202	15	-0.484429322666287
1202	16	-0.35841847429513507
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2211	12	0.13790930220589104
2211	13	0.17710019603892135
2211	14	0.4218086545816888
2211	15	0.10552159552256057
2211	16	0.3346477842614277
2211	17	0.5653412584065833
2211	18	0.5919508108915466
2211	19	0.29754270032886154
2211	20	0.4523802949109168
2211	21	0.35224984790412606
2211	22	0.3387089989218097
2211	23	-1.8436361692691747
2211	24	-0.5748488072640257
2211	25	-0.20199486330129446
2212	1	0.14319107310501522
2212	2	-1.4312711097636388
2212	3	-0.23021903843137537
2212	4	0.3782070656690806
2212	5	0.4195632536941005
2212	6	0.4070963274247461
2212	7	0.3463751192245397
2212	8	0.2770064723270025
2212	9	0.727032195753921
2212	10	0.6286357799521838
2212	11	0.32268062602014425
2212	12	0.22165769505683386
2212	13	0.12200201033164149
2212	14	0.4906506192224782
2212	15	0.25342564148412805
2212	16	0.36778352995929
2212	17	0.6690424309979903
2212	18	0.7058387988760586
2212	19	0.45504861734012536
2212	20	0.4330462434280946
2212	21	0.3100141635964633
2212	22	0.4325061757448907
2212	23	0.6172888729124537
2212	24	0.3305185879768828
2212	25	0.44674702373302466
2213	1	0.4725552019497776
2213	2	0.21233534649865504
2213	3	0.3468233621123886
2213	4	0.5224793771974205
2213	5	0.6142729599488064
2213	6	0.5327998211908991
2213	7	0.4359763676737032
2213	8	0.3705266914935521
2213	9	0.6331133104781301
2213	10	0.7483306665078331
2213	11	0.4029142705082336
2213	12	0.35450414382383144
2213	13	0.20485823332362685
2213	14	-1.150786320011747
2213	15	-0.212877515347472
2213	16	0.13216397815059366
2213	17	0.6170018645024697
2213	18	0.38900707523309563
2213	19	0.23258920873156086
2213	20	0.32059109658786156
2213	21	0.4329649277243064
2213	22	0.37165028214502627
2213	23	0.6672242982098311
2213	24	0.35127080702794444
2213	25	0.2963393052330545
2214	1	0.5541314556552912
2214	2	0.43827957952440144
2214	3	0.5480332048906682
2214	4	0.784549039578323
2214	5	0.7542559269874758
2214	6	0.5309748576166626
2214	7	0.4823540505068351
2214	8	0.33982484355129117
2214	9	0.5026481723676923
2214	10	0.3487972257132057
2214	11	0.3151025304192956
2214	12	0.2222144144679561
2214	13	0.1940153823054871
2214	14	0.4839136737811671
2214	15	0.3176808899245884
2214	16	0.43181314594048814
2214	17	0.4417121830929247
2214	18	0.6165394947418577
2214	19	0.3700342265783618
2214	20	0.5037203529064982
2214	21	0.4864603720533225
2214	22	0.5157124474094275
2214	23	0.5568103867762275
2214	24	0.5682481976862661
2214	25	0.4816113497879897
2215	1	0.4492959158989649
2215	2	0.2554193579513999
2215	3	0.443743934211124
2215	4	0.6352325834785688
2215	5	0.6649541429375958
2215	6	0.5374867967642984
2215	7	0.48237280243779423
2215	8	0.36745859059512753
2215	9	0.6147437069264541
2215	10	0.6833788443408437
2215	11	0.4209699259652311
2215	12	0.2474106079979208
2215	13	0.20947205061296614
2215	14	0.3919337677648258
2215	15	0.2928869218282415
2215	16	0.39261848491033646
2215	17	0.5807504548777942
2215	18	0.7854871545008706
2215	19	0.3484185597420364
2215	20	-0.5122390855747199
2215	21	0.013053061616517425
2215	22	-0.6160227140737433
2215	23	0.24944799760083924
2215	24	0.19782146136434875
2215	25	0.35939403642523615
2216	1	0.36503608157781997
2216	2	-0.16314114499228438
2216	3	0.34302896052575704
2216	4	0.5612519112327377
2216	5	0.5540746804952761
2216	6	0.5115421993953291
2216	7	0.4288921540420887
2216	8	0.3251879818002621
2216	9	0.6682195371155046
2216	10	0.429374071425336
2216	11	0.3650503013613431
2216	12	0.007452352940369167
2216	13	0.14437326051284316
2216	14	0.14634688369424964
2216	15	0.16613869577147966
2216	16	0.3494984262824019
2216	17	0.32018906634772293
2216	18	0.73465603794412
2216	19	0.4107796448214458
2216	20	-0.7185908310423486
2216	21	-1.0214669799549654
2216	22	-1.0754498003933577
2216	23	-1.4697334276343241
2216	24	-1.0990123504972118
2216	25	-0.8478524500180171
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10637.0
3	1518.0
4	1325.0
5	993.0
6	1161.0
7	1438.0
8	553.0
9	968.0
10	2105.0
11	4425.0
12	8311.0
13	13578.0
14	19072.0
15	24521.0
16	28636.0
17	31457.0
18	32842.0
19	33868.0
20	34130.0
21	34617.0
22	35799.0
23	37219.0
24	39251.0
25	42077.0
26	46448.0
27	53154.0
28	61840.0
29	75360.0
30	93898.0
31	118828.0
32	156013.0
33	209792.0
34	289533.0
35	409571.0
36	610428.0
37	991440.0
38	1862166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15378516802702	21.806736308776006	14.11786599900131	22.921612524195663
2	15.141427161010165	21.820227567771134	40.71334918060243	22.324996090616274
3	17.59769775881094	27.204806841393896	30.565044220666877	24.63245117912828
4	12.624462626157475	18.035070977153982	38.952664424031745	30.387801972656796
5	13.301020781484594	37.663826582924486	35.33309930969201	13.702053325898914
6	29.853661318523873	39.2067237322039	18.15780610295979	12.781808846312446
7	24.86397817725573	33.028694568237185	24.93672929916728	17.17059795533981
8	22.71295776541602	37.89663619357411	21.833600728346607	17.55680531266326
9	24.11052189702493	17.389703826410347	22.72829453445606	35.77147974210866
10	14.658668534807395	30.15992682539195	34.33827400245323	20.84313063734743
11	31.34846759451788	26.34039558138945	24.283128798533177	18.028008025559494
12	20.241835554933406	27.871896740591623	32.28715560384638	19.599112100628595
13	25.624942384349424	25.013448279564283	27.450113639350647	21.91149569673565
14	20.37770485393613	23.831150246275325	28.618199752002987	27.17294514778556
15	22.24587465744456	31.358900311158962	24.599152031182197	21.79607300021428
16	21.81148663920038	31.654868824319603	25.90771749373734	20.625927042742674
17	20.01514960699919	30.669212590842083	28.837906613229425	20.477731188929305
18	20.09421521051327	30.612093383551333	29.43810609801322	19.855585307922183
19	20.720646486851617	29.19238027801808	29.652003365606816	20.43496986952348
20	21.966851659110684	28.932267888407782	28.80532813777461	20.295552314706928
21	22.433623844937685	29.44826991099479	27.788639197623567	20.32946704644396
22	21.1872072085078	29.030386923881757	29.516066133513384	20.266339734097063
23	21.524941588114878	29.483392075229105	28.55155919854415	20.440107138111863
24	21.20158880432034	28.363352350458126	29.04895740270184	21.386101442519696
25	21.984005177223843	28.70850959891139	28.241859728110597	21.06562549575417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14847.0
1	14847.0
2	12512.5
3	10178.0
4	10178.0
5	10178.0
6	11034.0
7	11890.0
8	11890.0
9	11890.0
10	17534.0
11	23178.0
12	23178.0
13	23178.0
14	38320.5
15	53463.0
16	53463.0
17	53463.0
18	84698.0
19	115933.0
20	115933.0
21	115933.0
22	167019.5
23	218106.0
24	218106.0
25	218106.0
26	281888.0
27	345670.0
28	345670.0
29	345670.0
30	413746.5
31	481823.0
32	481823.0
33	481823.0
34	541393.0
35	600963.0
36	600963.0
37	600963.0
38	641088.0
39	681213.0
40	681213.0
41	681213.0
42	672239.5
43	663266.0
44	663266.0
45	663266.0
46	629615.5
47	595965.0
48	595965.0
49	595965.0
50	560880.0
51	525795.0
52	525795.0
53	525795.0
54	472078.5
55	418362.0
56	418362.0
57	418362.0
58	357337.0
59	296312.0
60	296312.0
61	296312.0
62	241864.5
63	187417.0
64	187417.0
65	187417.0
66	143636.0
67	99855.0
68	99855.0
69	99855.0
70	72921.0
71	45987.0
72	45987.0
73	45987.0
74	32455.0
75	18923.0
76	18923.0
77	18923.0
78	12844.5
79	6766.0
80	6766.0
81	6766.0
82	4495.5
83	2225.0
84	2225.0
85	2225.0
86	1443.0
87	661.0
88	661.0
89	661.0
90	402.5
91	144.0
92	144.0
93	144.0
94	84.0
95	24.0
96	24.0
97	24.0
98	15.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14537812706911937
2	0.2824705497647893
3	0.34451183730050644
4	0.37392701051048055
5	0.3811977622323939
6	0.33735180768603346
7	0.33895727824391786
8	0.35698652807211406
9	0.34246347831286084
10	0.37604918423641975
11	0.2903687267621977
12	0.3388096487673308
13	0.30991117872541135
14	0.3191380210121034
15	0.3594962291740943
16	0.3084164302749673
17	0.3596992197044015
18	0.32701774432493835
19	0.3406550172246692
20	0.30280651016465854
21	0.3434968846489703
22	0.2956833879193323
23	0.2787613591655391
24	0.3169235788632973
25	0.3109261313769475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5418972.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.60993559565716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45877634414242	61.433951515846786
2	11.531623188106243	16.976840803797746
3	2.729781528803673	6.0281712757635875
4	0.9280483459689867	2.7325431590573306
5	0.4172350739362003	1.5356323460346477
6	0.23259130849356008	1.027261874299235
7	0.14815551334056506	0.7634002456598364
8	0.10063462816557305	0.5926166798368591
9	0.06909462810652374	0.4577446012434386
>10	0.35217708809919146	4.495481692345829
>50	0.019524613102653552	0.9838909337726857
>100	0.011201098160302169	1.4808675069961494
>500	6.286123102695282E-4	0.3319183437792267
>1k	4.023080105560233E-4	0.59723765753455
>5k	1.2572125326440557E-4	0.5624413640319912
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6862	0.1266291835425612	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6114	0.11282582748166996	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6099	0.11254902221306919	No Hit
ATTCCATTCCATTCCATTCCATTCC	5961	0.1100024137419422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.6907369146768055E-5	0.0
5	0.0	0.0	0.0	3.6907369146768055E-5	0.0
6	0.0	0.0	0.0	3.6907369146768055E-5	0.0
7	0.0	0.0	1.8453684573384028E-5	3.6907369146768055E-5	0.0
8	0.0	1.8453684573384028E-5	1.8453684573384028E-5	5.5361053720152086E-5	0.0
9	0.0	1.8453684573384028E-5	1.8453684573384028E-5	7.381473829353611E-5	0.0
10	0.0	1.8453684573384028E-5	1.8453684573384028E-5	9.226842286692014E-5	0.0
11	0.0	1.8453684573384028E-5	1.8453684573384028E-5	9.226842286692014E-5	0.0
12	0.0	1.8453684573384028E-5	1.8453684573384028E-5	9.226842286692014E-5	0.0
13	0.0	1.8453684573384028E-5	1.8453684573384028E-5	9.226842286692014E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTACT	70	0.0014855497	10.863165	4
AGCGCGC	70	0.0014944184	10.855416	10
CGGTCGT	70	0.0014944184	10.855416	10
GGTATCA	4130	0.0	10.725969	1
GGACCGA	155	4.0792656E-8	9.805166	6
CCGGTCG	90	0.0011156013	9.498931	9
GTGGTAT	1960	0.0	9.166561	1
TGGTATC	1875	0.0	8.719339	2
GTATTAG	985	0.0	8.589234	1
CTGCACG	190	1.0038639E-7	8.504715	4
GTTCTAG	700	0.0	8.283855	1
TAGACTG	685	0.0	8.186944	5
TGGACCG	165	9.684136E-6	8.065002	5
TAGGACT	630	0.0	7.996497	4
GTAGGAC	345	0.0	7.9897885	3
AGGACGG	155	3.8753802E-5	7.972088	5
CGGGACT	120	0.0016827811	7.921058	4
TCTATAC	690	0.0	7.8520336	3
ACAGTCC	620	0.0	7.813636	8
GTATCAA	6985	0.0	7.784493	1
>>END_MODULE