FastQCFastQC Report
Wed 25 May 2016
SRR1295236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418972
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT177390.32734991064725927No Hit
GGTATCAACGCAGAGTACTTTTTTT146570.2704756547920897No Hit
TATCAACGCAGAGTACTTTTTTTTT102600.18933480372292014No Hit
GTGGTATCAACGCAGAGTACATGGG75910.14008191959655816No Hit
ATTCCATTCCATTCCATTCCATTCC67230.12406412138686083No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63640.11743924862501598No Hit
GAATGGAATGGAATGGAATGGAATG58710.10834158213033764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.011.5340351
GCAGCGT4700.011.3212491
GTCGCGC600.0058796211.0831398
AGCGTCA5300.010.7557193
GTGGTAT22600.010.5948871
CAGCGTC5600.010.3491792
TGGTATC21900.010.1516312
AACCGTG1054.1084546E-59.9525757
CGGATAG900.00111350459.5008865
GTACCGC800.00451778539.5001856
ATACCGT1451.7081475E-69.1725926
AATACCG1356.8474037E-69.1490015
GGACCGT1151.1114101E-49.0871336
GTCCTAT4350.08.9556851
ATTAGAC4550.08.7700483
ACACCGC1107.148172E-48.6365326
TCGGAAC1000.0029067888.55000913
GTCCTAG4900.08.5321951
CTACACT8100.08.4451544
TAGAACC3500.08.4149924
Produced by FastQC (version 0.11.5)