FastQCFastQC Report
Wed 25 May 2016
SRR1295235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988443
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN78840.11281482871077292No Hit
GTATCAACGCAGAGTACTTTTTTTT71180.10185387503339441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG907.481558E-611.6096239
TTCGCGG855.3384927E-511.17447714
GGTATCA45450.09.5792931
ACGTCGC1106.852407E-59.4989196
TGTACCG2550.09.3189585
GTTCTAG8000.08.9119591
CTTCGCG1304.38208E-58.76779313
GGTCGAA1201.7693451E-48.70659211
GTGTAGA11800.08.7004891
TGGTATC23400.08.6518592
GTACGAC1107.111882E-48.6410293
GTATTAG10500.08.6007471
CGCCTAA1451.6950362E-58.51572114
GTCCTAA6250.08.3653591
TAGACAG10400.08.3171715
GTGGTAT25650.08.3016141
TACGTCG1502.5709187E-58.2379595
GTCTAAG6350.08.2336211
GCGTCAG2105.756374E-88.1480771
GCACCGT2355.5442797E-98.0841866
Produced by FastQC (version 0.11.5)