FastQCFastQC Report
Wed 25 May 2016
SRR1295235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988443
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT198070.2834250776603601No Hit
GGTATCAACGCAGAGTACTTTTTTT164660.23561757604662442No Hit
TATCAACGCAGAGTACTTTTTTTTT113330.16216773893698497No Hit
GTGGTATCAACGCAGAGTACATGGG77020.11021052901197019No Hit
ACGCAGAGTACTTTTTTTTTTTTTT76970.11013898231694813No Hit
ATTCCATTCCATTCCATTCCATTCC70240.10050879716697982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG954.9112714E-914.0001849
AGCGTCA6200.011.7993023
GGTATCA60500.011.0883971
CGCCTAA1303.850928E-710.23024514
GCAGCGT7250.09.5676291
TATACCG1502.64974E-79.5008055
CGACCGC1304.243904E-69.50012510
CAGCGTC7750.09.439242
TACGACT1252.7550406E-59.1206424
ACTCGCG1701.687713E-78.9412958
ATCGACC1856.586015E-88.7298458
GTCCTAG6800.08.6636771
TAGATCG1107.1441784E-48.6370955
GCGTCAG8550.08.5561584
GTGGTAT27900.08.5144231
CTACACT9050.08.5033614
TCTATAC8650.08.4573043
GTCTAAG6250.08.3618461
GTTCTAG8300.08.3572651
TCTACAC9800.08.3373813
Produced by FastQC (version 0.11.5)