FastQCFastQC Report
Wed 25 May 2016
SRR1295234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921246
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69610.14144791786470337No Hit
GTGGTATCAACGCAGAGTACATGGG69300.14081799609285942No Hit
GGTATCAACGCAGAGTACTTTTTTT57660.11716544956297653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN55340.11245119630272496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17450.011.4944071
GGTATCA39200.011.1792951
TGGTATC17050.011.0936652
TCGACCG600.00588306811.0822329
TACGTGA700.001494141310.85562110
GTCTTAG3850.09.6294931
GTATTAG5700.09.1724931
GACGGAT1151.11328576E-49.0856837
GATCCGT1054.5026012E-49.0462596
GTGTACG850.0074008528.9468591
AGTGCGC850.00744745558.9400158
GTCGTAG1201.7531107E-48.7138681
GCGTGCG1000.00290932628.549159
TATTCCG2252.4265319E-98.4491225
ACAGCGT1701.6166468E-68.3812658
TTAGTAC3650.08.3332953
GTCCTAG4500.08.2385661
GTTCTAG7050.08.2250831
TTACACT8150.08.1641124
TAGGACG1752.423063E-68.1474514
Produced by FastQC (version 0.11.5)